外源水杨酸对镉胁迫下小报春幼苗生长及生理特性的影响

doi:10.11733/j.issn.1007-0435.2020.05.020

草地学报 ›› 2020, Vol. 28 ›› Issue (5): 1346-1354.DOI: 10.11733/j.issn.1007-0435.2020.05.020

外源水杨酸对镉胁迫下小报春幼苗生长及生理特性的影响

贾茵, 刘才磊, 兰晓悦, 陈筱溪, 赵健, 潘远智

四川农业大学风景园林学院, 四川成都 611130

收稿日期:2020-04-27 修回日期:2020-05-28 出版日期:2020-10-15 发布日期:2020-09-19
通讯作者: 潘远智
作者简介:贾茵(1983-),女,山西太原人,博士,副教授,主要从事园林植物资源与应用研究,E-mail:jiayin_cn@163.com
基金资助:
国家自然科学基金项目（31670622）资助

Effects of Exogenous Salicylic Acid on the Growth and Physiological Characteristics of Primula forbesii Franch. Seedlings under Cadmium Stress

JIA Yin, LIU Cai-lei, LAN Xiao-yue, CHEN Xiao-xi, ZHAO Jian, PAN Yuan-zhi

College of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China

Received:2020-04-27 Revised:2020-05-28 Online:2020-10-15 Published:2020-09-19

摘要/Abstract

摘要： 为了探究叶面喷施外源水杨酸（Salicylic acid，SA）对小报春（Primula forbesii Franch.）镉（Cadmium，Cd）胁迫的缓解作用，本研究以小报春幼苗为试验材料，采用盆栽试验方法，在土壤Cd胁迫（150 mg·kg^-1）下，测定不同浓度（0，10，100，1 000 μmol·L^-1） SA处理下的小报春植株生长和生理特性指标。结果表明，低浓度SA （10，100 μmol·L^-1）能够使小报春株高、冠幅和地上部生物量增加，促进光合作用；低浓度SA能降低过氧化氢和丙二醛含量，提高超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化物酶（Peroxidase，POD）和过氧化氢酶（Catalase，CAT）活性，促进可溶性糖和可溶性蛋白的产生；同时低浓度SA还能显著降低小报春幼苗叶片中的Cd含量，增加叶片中的钾和锌含量，降低钙和镁含量。而高浓度SA （1 000 μmol·L^-1）缓解作用下降，甚至对植株生长产生抑制作用。本研究表明，100 μmol·L^-1外源SA处理可以显著缓解150 mg·kg^-1土壤Cd胁迫对小报春幼苗的毒害作用，并降低植株镉积累。

关键词: 外源水杨酸, 镉, 小报春, 抗氧化系统, 光合参数, 营养元素

Abstract: In order to explore the effects of foliar application of exogenous salicylic acid (SA) on Primula forbesii Franch. under cadmium (Cd) stress,a pot experiment was performed with P. forbesii seedlings as materials. The effects of different concentrations of exogenous salicylic acid (0,10,100,1 000 μmol·L^-1) on P. forbesii growth and physiological characteristics under 150 mg·kg^-1 soil Cd stress were investigated. The results showed that low concentrations of SA (10,100 μmol·L^-1) increased the plant height,crown width,aboveground biomass and promoted photosynthesis. Furthermore,exogenous SA application reduced the content of hydrogen peroxide and malondialdehyde in P. forbesii seedlings under Cd stress. The activities of SOD,POD and CAT,and the contents of the soluble sugar and soluble protein in Cd-treated P. forbesii seedlings were increased by SA application. Meanwhile,SA application also decreased the contents of Cd,Ca and Mg but increased K and Zn contents in leaves of P. forbesii seedlings under Cd stress. However,1 000 μmol·L^-1 SA treatment was found to inhibite the plant growth. This study showed that 100 μmol·L^-1exogenous SA treatment alleviated the toxic effects of 150 mg·kg^-1 soil Cd stress on P. forbesii seedlings,and reduced Cd accumulation.

Key words: Exogenous salicylic acid, Cadmium, Primula forbesii Franch., Antioxidant system, Photosynthetic parameters, Nutrient elements

中图分类号:

Q945.78

贾茵, 刘才磊, 兰晓悦, 陈筱溪, 赵健, 潘远智. 外源水杨酸对镉胁迫下小报春幼苗生长及生理特性的影响[J]. 草地学报, 2020, 28(5): 1346-1354.

JIA Yin, LIU Cai-lei, LAN Xiao-yue, CHEN Xiao-xi, ZHAO Jian, PAN Yuan-zhi. Effects of Exogenous Salicylic Acid on the Growth and Physiological Characteristics of Primula forbesii Franch. Seedlings under Cadmium Stress[J]. Acta Agrestia Sinica, 2020, 28(5): 1346-1354.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2020.05.020

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2020/V28/I5/1346

参考文献

[1] 环境保护部,国土资源部. 全国土壤污染状况调查公报[N]. 中国国土资源报,2014-04-18(2)
[2] 宋伟,陈百明,刘琳. 中国耕地土壤重金属污染概况[J]. 水土保持研究,2013,20(2):293-298
[3] Ali B,Deng X,Hu X,et al. Deteriorative effects of cadmium stress on antioxidant system and cellular structure in germinating seeds of Brassica napus L.[J]. Journal of Agricultural Science&Technology,2018,17(1):63-74
[4] 王学华,戴力. 作物根系镉滞留作用及其生理生化机制[J]. 中国农业科学,2016,49(22):4323-4341
[5] Serrano-Martinez F,Casas J L. Effects of extended exposure to cadmium and subsequent recovery period on growth,antioxidant status and polyamine pattern in in vitro cultured carnation[J]. Physiology and Molecular Biology of Plants,2011,17(4):327-338
[6] Fu X,Dou C,Chen Y,et al. Subcellular distribution and chemical forms of cadmium in Phytolacca americana L.[J]. Journal of Hazardous Materials,2011,186(1):103-107
[7] Zanganeh R,Jamei R,Rahmani F. Impacts of seed priming with salicylic acid and sodium hydrosulfide on possible metabolic pathway of two amino acids in maize plant under lead stress[J]. Molecular Biology Research Communications,2018,7(2):83-88
[8] Yan S,Dong X. Perception of the plant immune signal salicylic acid[J]. Current Opinion in Plant Biology,2014(20):64-68
[9] 耿志卓,丁立人,逯亚玲,等. 外源水杨酸对不同水分胁迫下分枝期紫花苜蓿生长和生理特性的影响[J]. 草地学报,2016,24(2):369-376
[10] 逯亚玲,王灵婧,王宁,等. 外源水杨酸对NaCl胁迫下紫花苜蓿幼苗生长和生理特性的影响[J]. 草地学报,2017,25(6):1265-1273
[11] Ahmad B,Jaleel H,Sadiq Y,et al. Response of exogenous salicylic acid on cadmium induced photosynthetic damage,antioxidant metabolism and essential oil production in peppermint[J]. Plant Growth Regulation,2018,86(2):273-286
[12] 陈封怀,胡启明. 中国植物志(第59卷第2分册)[M]. 北京:科学出版社,1990:7-8
[13] 范眸天. 云南野生花卉[M]. 云南:云南科学技术出版社,2001:85-88
[14] 贾茵,刘才磊,兰晓悦,等. 镉胁迫对小报春幼苗生长及生理特性的影响[J]. 西北植物学报,2020,40(3):0454-0462
[15] 彭运生,刘恩. 关于提取叶绿素方法的比较研究[J]. 北京农业大学学报,1992,18(3):247-250
[16] Elstner E F,Heupel A. Inhibition of nitrite formation from hydroxylammonium chloride:a simple assay for superoxide dismutase[J]. Analytical Biochemistry,1976,70(2):616-620
[17] Liu J,Lu B,Xu L L. An improved method for the determination of hydrogen peroxide in leaves[J]. Progress in Biochemistry and Biophysics,2000,27(5):548-551
[18] Heath R L,Packer L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophysics,1968,125(1):189-198
[19] 陈建勋,王晓峰. 植物生理学实验指导[M].第二版. 广州:华南理工大学出版社,2006:64-66
[20] Giannopolitis C N,Ries S K. Superoxide dismutase:I. Occurrence in higher plants[J]. Plant Physiology,1977,59:309-314
[21] Aebi H. Catalase in vitro[J]. Methods in Enzymology,1984,105:121-126
[22] Beffa R,Martin H V,Pilet P E. In vitro oxidation of indoleacetic acid by soluble auxin-oxidases and peroxidases from maize roots[J]. Plant Physiology,1990,94(2):485-491
[23] 刘海英,王华华,崔长海,等. 可溶性糖含量测定(蒽酮法)实验的改进[J]. 实验室科学,2013,16(2):19-20
[24] 李合生. 植物生理生化实验原理与技术[M]. 北京:高等教育出版社,2000:167-169
[25] Wang X S,Han J G. Changes of proline content,activity,and active isoforms of antioxidative enzymes in two alfalfa cultivars under salt stress[J]. Agricultural Sciences in China,2009,8(4):431-440
[26] Kranten A,Yordanova R,Janda T,et al. Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants[J]. Journal of Plant Physiology,2008,165(9):920-931
[27] Kováčik J,Grúz J,Bačkor M,et al. Salicylic acid-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants[J]. Plant Cell Reports,2009,28(1):135-143
[28] 唐艳萍,文涛,孙歆,等. 水杨酸对植物光合作用影响的研究进展[J]. 西北植物学报,2015,35(8):1701-1708
[29] Poór P,Patyi G,Takács Z,et al. Salicylic acid-induced ROS production by mitochondrial electron transport chain depends on the activity of mitochondrial hexokinases in tomato (Solanum lycopersicum L.)[J]. Journal of Plant Research,2019,132(2):273-283
[30] 俞萍,高凡,刘杰,等. 镉对植物生长的影响和植物耐镉机制研究进展[J]. 中国农学通报,2017,33(11):89-95
[31] Dong Y J,Wang Z L,Zhang J W,et al. Interaction effects of nitric oxide and salicylic acid in alleviating salt stress of Gossypium hirsutum L.[J]. Journal of Soil Science and Plant Nutrition,2015,15(3):561-573
[31] Lu Q,Zhang T,Zhang W,et al. Alleviation of cadmium toxicity in Lemna minor by exogenous salicylic acid[J]. Ecotoxicology and Environmental Safety,2018,147:500-508
[33] Manquián-Cerda K,Cruces E,Escudey M,et al. Interactive effects of aluminum and cadmium on phenolic compounds,antioxidant enzyme activity and oxidative stress in blueberry (Vaccinium corymbosum L.) plantlets cultivated in vitro[J]. Ecotoxicology & Environmental Safety,2018,150:320-326
[34] 王瑜,袁庆华,苗丽宏. 不同狼尾草幼苗对镉胁迫的响应[J]. 草地学报,2015,23(1):130-136
[35] 张思宇,田佳源,祁小旭,等. 外来入侵植物黄顶菊生长及生理生态特性对镉胁迫的响应[J]. 农业资源与环境学报,2020,37(3),445-452
[36] Liu Z,Ding Y,Wang F,et al. Role of salicylic acid in resistance to cadmium stress in plants[J]. Plant Cell Reports,2016,35(4):719-731
[37] Gu C S,Yang Y H,Shao Y F,et al. The effects of exogenous salicylic acid on alleviating cadmium toxicity in Nymphaea tetragona Georgi[J]. South African Journal of Botany,2018,114:267-271
[38] 李焕勇,唐晓倩,杨秀艳. NaCl处理对西伯利亚白刺幼苗中矿质元素含量的影响[J]. 植物生理学报,2017,53(12):2125-2136
[39] 李君,葛跃,王明新,等. EGTA对Cd胁迫下蓖麻Cd积累和营养元素吸收的影响[J]. 西北植物学报2015,35(09),1855-1860
[40] Shi Q H,Zhu Z J. Effects of exogenous salicylic acid on manganese toxicity,element contents and antioxidative system in cucumber[J]. Environmental and Experimental Botany,2008,63:317-326
[41] Babasheikhali M M,Jabbarzadeh Z,Amiri J,et al. Impact of salicylic acid and nitric oxide on improving growth and nutrients uptake of rose in alkaline soil conditions[J]. Journal of Plant Nutrition,2020,43:667-681
[42] 周媛. 水杨酸对铝胁迫下栝楼生长的调控机理研究[D]. 金华:浙江师范大学,2012:29-41
[43] 王小红,郭军康,贾红磊,等. 外源水杨酸缓解镉对番茄毒害作用的研究[J]. 农业环境科学学报,2019,38(12):2705-2714
[44] 李雷,刘彤,邓群仙,等. 外源水杨酸对镉污染下酸枣幼苗生长及镉积累的影响[J]. 四川农业大学学报,2019,37(3):359-365

外源水杨酸对镉胁迫下小报春幼苗生长及生理特性的影响

Effects of Exogenous Salicylic Acid on the Growth and Physiological Characteristics of Primula forbesii Franch. Seedlings under Cadmium Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张旭辉, 马绍英, 马蕾, 杨宁, 王娜, 张维霞, 连荣芳, 李胜, 柴强. 接种根瘤菌对重茬豌豆植株生长及光合特性的影响[J]. 草地学报, 2021, 29(6): 1234-1241.
[2]	张杨杨, 李希铭, 高鹏, 宋桂龙. 不同浓度镉胁迫下6种草本植物的耐性及富集特征的比较[J]. 草地学报, 2021, 29(6): 1265-1276.
[3]	朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报, 2021, 29(4): 635-643.
[4]	孔海明, 李开源, 高双红, 孙娈姿. 根瘤菌对紫花苜蓿生物修复镉污染功能的影响[J]. 草地学报, 2021, 29(12): 2763-2768.
[5]	赵利清, 彭向永, 刘俊祥, 毛金梅, 孙振元. GSH对铅胁迫下多年生黑麦草幼苗抗氧化系统的调控[J]. 草地学报, 2020, 28(6): 1527-1534.
[6]	尉欣荣, 张智伟, 周雨, 刘鹏, 姚宏斌, 苗彦军, 付娟娟. 褪黑素对低温和干旱胁迫下多年生黑麦草幼苗生长和抗氧化系统的调节作用[J]. 草地学报, 2020, 28(5): 1337-1345.
[7]	徐美蓉, 李敏权, 曹素芳, 蒋晶晶, 李雪萍, 孙新荣, 陈爱昌, 李继平, 漆永红. 根腐病对党参细胞结构和生理特性的影响[J]. 草地学报, 2020, 28(4): 915-922.
[8]	卜和申, 谢燕, 徐倩, 陈良, 陈云, 胡龙兴. 哈茨木霉的耐镉性及其对狗牙根耐镉能力的影响[J]. 草地学报, 2020, 28(1): 64-71.
[9]	刘霄霏, 李惠英, 陈良, 徐倩, 胡龙兴, 王慧彬, 马向明. 外源硒对镉胁迫下黑麦草生长和生理的影响[J]. 草地学报, 2020, 28(1): 72-79.
[10]	鲜靖苹, 王勇, 马晖玲. 外源NO缓解草地早熟禾镉胁迫的转录组分析[J]. 草地学报, 2019, 27(6): 1467-1476.
[11]	鲜靖苹, 王勇, 马晖玲. 一氧化氮信号途径参与草地早熟禾耐镉机制的研究[J]. 草地学报, 2019, 27(6): 1577-1586.
[12]	马瑞丽, 陈垣, 郭凤霞, 武睿, 焦旭升. 甘肃贝母生物量和营养物质生殖分配研究[J]. 草地学报, 2019, 27(5): 1291-1300.
[13]	普布卓玛, 罗艺岚, 高金柱, 孙鹏越, 赵东豪, 苗彦军, 付娟娟, 呼天明. 2,4表-油菜素内酯对低温胁迫下西藏野生垂穗披碱草幼苗抗氧化保护和渗透调节的影响[J]. 草地学报, 2019, 27(3): 547-552.
[14]	邵将, 陈瑶, 刘大林, 陈鸣晖, 吴亚, 黄玉婷. 硅对镉胁迫下不同狼尾草属牧草生理代谢的影响[J]. 草地学报, 2018, 26(5): 1223-1228.
[15]	见妮, 刘璐, 屈佳欣, 李凯, 王佺珍. 超声波对镉胁迫鸭茅和冰草种子萌发的影响[J]. 草地学报, 2018, 26(2): 427-434.