Abstract：White clover (Trifolium repens ‘Haifa’) was selected as experimental material in this study,and effects of seeds soaking with gamma-aminobutyric acid (GABA) on germination and tolerance to aluminium (Al3+) stress were studied. The results showed that 1 mmol·L-1 Al3+ solution significantly inhibited seeds germination,and the damage becomes more obvious with the increase of Al3+ concentration. The germination and root length of seeds soaking with GABA significantly increased under Al3+ stress with the most obvious effect under 4 mmol·L-1 Al3+ stress. The antioxidant enzyme activity and non-enzymatic antioxidants content of seeds soaking with GABA significantly increased as well,thereby reducing oxidative damage effectively and improving cell membrane stability during germination under Al3+ stress. In addition,seeds soaking with GABA also significantly increased genes expression of dehydrins under Al3+ stress. Therefore,GABA-induced Al3+ tolerance could be related to the enhancement of antioxidant defense to reduce oxidative damage caused by stress and the up-regulation of dehydrins genes expression during white clover seeds germination.
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FU Jing-yan, LIANG Lin-lin, ZHOU Min, LI Zhou. Effects of seeds soaking with γ-aminobutyric acid on germination and aluminium tolerance of white clover. Acta Agrestia Sinica, 2020, 28(5): 1275-1284.
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2020, Vol. 28 
