关键词: 野生羌活, 休眠, 破除休眠, 胚后熟, 激素处理, 变温处理, 层积处理

Abstract: Wild Notopterygium incisum Ting ex H.T.Chang and N.forbesii Boiss.have been facing more and more serious over-exploitation under the pressure of population and economy development owing to wild resource exploitation,vegetation destruction,habitat fragmentation,and lands reclamation.The short growth phase and long growth cycle of those two species make this crisis more rigorous in plateau regions with high altitude and cold weather,and very low progenitive rate is the major obstacle to artificial domestication and cultivation.Characteristic of seed dormancy and breaking technique for rare and precious species of wild N.incisium were studied in both laboratory and fields.The results of morphological dissection,physiology and biochemical measurement of N.incisium seeds indicate the seed embryo had the property of after-ripening with the dormancy period of 8-10 month,the seed dormancy could be broken down only by embryo’s after-ripening.The seed harvested in autumn with no-embryo or the embryo at original state had no germination capacity and after-ripening was necessary.The technology for dormancy breaking was found effective as follow:seeds flushed by flowing water could make the ABA content of seeds decreased to the level needed for seed germination;seeds treated with gibberellin(GA) or both GA and 6-benzyl adenine(BA) could accelerate the germination course and improve the germination rate.The temperature-changing stratification(15～25℃/2～5℃)could shorten the course of dormancy breaking;temperature-warming before stratification could accelerate the growth of embryo,low temperature was the necessary condition for seedling sprouting.The seed germination rate was increased to 19.8% through 2-3 months’ warm-temperature and 2-3 months’ low temperature stratification.

Key words: Wild Notopterygium incisium, Seed dormancy, Dormancy breaking, Afterripening of embryo, Hormone treatment, Temperature change treatment, Stratification treatment