盐胁迫条件下苦马豆幼苗根差异表达基因及功能注释

doi:10.11733/j.issn.1007-0435.2011.02.025

›› 2011, Vol. 19 ›› Issue (2): 325-330.DOI: 10.11733/j.issn.1007-0435.2011.02.025

盐胁迫条件下苦马豆幼苗根差异表达基因及功能注释

王玉祥¹, 贾广云¹, 李道丰², 张博¹, 王涛²

1. 新疆农业大学草地资源与生态重点实验室, 乌鲁木齐, 830052;
2. 中国农业大学农业生物技术国家重点实验室, 北京, 100193

收稿日期:2009-04-22 修回日期:2011-02-28 出版日期:2011-04-15 发布日期:2011-04-15
通讯作者: 张博,E-mail:xjauzb@126.com;王涛,E-mail:wangt@cau.edu.cn
作者简介:王玉祥(1980- ),男,安徽亳州人,硕士,研究方向为牧草遗传育种,E-mail:wyx9868@163.com
基金资助:
新疆维吾尔自治区高技术研究发展计划(200711101);国家十一五科技支撑子项目(2008BADB3B06-02);国家高技术研究863发展计划(2009AA10Z108)资助

Differentially Expressed Genes of Swainsonine Roots under Salinity Stress and Its Annotation

WANG Yu-xiang¹, JIA Guang-yun¹, LI Dao-feng², ZHANG Bo¹, WANG Tao²

1. Key Laboratory of Grassland Resource and Ecology of Xinjiang, Xinjiang Agricultural University, Urumqi, Xinjinag 830052, China;
2. State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, China

Received:2009-04-22 Revised:2011-02-28 Online:2011-04-15 Published:2011-04-15

摘要/Abstract

摘要： 苦马豆(Swainsona salsula Taubert)是新疆重要的耐盐抗旱豆科植物,通过染色体压片技术确定供试材料为二倍体,采用截形苜蓿(Medicago truncatula)基因组芯片(61278个探针),对苦马豆幼苗根部在未经盐处理和在180 mM NaCl溶液中处理24 h后的表达水平进行了分析,找出控制盐胁迫的关键基因,为耐盐基因的筛选提供依据。结果表明:有4057个基因在不同处理条件下存在表达差异,其中155个上调基因和105个下调基因;利用Gene Ontology注释对差异表达的基因从分子功能、细胞组件、生物学过程3个水平进行功能分类,搜索NCBI数据库,在截形苜蓿和拟南芥(Arabidopsis thaliana)中找到有高相似性功能已知的部分基因,表明差异表达的基因涉及逆境胁迫、信号转导、电子传递、蛋白代谢等多个方面,这些基因是植物根部盐胁迫相关基因。

关键词: 苦马豆, 耐盐, 截形苜蓿基因组芯片, 功能注释

Abstract: Swainsona salsula Taubert is a valuable and drought resistant salt tolerant legume in Xinjiang.Key genes controlling salt stress are investigated here for providing genetic screening basis of salt tolerance.S.salsula is confirmed as a diploid legume determined by chromosome pressing technology.Affymetrix Medicago genome gene chip containing 61,278 probes are used to identify differentially expressed genes of roots both without and with salt treatment(180 mM NaCl,24 hours).Results show that 4,057 genes were identified to be significantly differentially expressed between the two treatments.There are 155 up-regulated genes and 105 down-regulated genes.Gene ontology annotation is used to analyze differentially expressed genes at molecular function,cell components and biological process levels.By searching NCBI database,some genes show high similarity with known genes of Medicago truncatula and Arabidopsis thaliana.Some differentially expressed genes show high identity with stress-inducible genes,signal transduction genes,electron transport or protein metabolism genes related to salt-stress in plant roots.

Key words: Swainsonine, Salt-tolerance, Medicago genome microarray, Annotation

中图分类号:

Q344.1

王玉祥, 贾广云, 李道丰, 张博, 王涛. 盐胁迫条件下苦马豆幼苗根差异表达基因及功能注释[J]. , 2011, 19(2): 325-330.

WANG Yu-xiang, JIA Guang-yun, LI Dao-feng, ZHANG Bo, WANG Tao. Differentially Expressed Genes of Swainsonine Roots under Salinity Stress and Its Annotation[J]. , 2011, 19(2): 325-330.

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参考文献

[1] 陈爱民,连瑞丽,孙杰.等.豆科模式植物——蒺藜苜蓿[J].植物生理与分子生物学,2006,42(5):997-1000
[2] Guo P G,Michael B,Li R H,et al.Response to drought stress at the reproductive growth stage using affymetrix Barley 1 genechip[J].Journal of Guangzhou University,2007,6(5):33-35
[3] 倪中福,孙其信,吴利民.普通小麦不同优势杂交种及其亲本之间基因表达差异比较研究[J].中国农业大学学报,2000,5(1):1-8
[4] 徐孟亮,陈荣军,ROCHA Pedro,等.一个新的水稻逆境响应基因OsMsrl的表达与克隆[J].作物学报,2008,34(10):1712-1718
[5] Yuanqing Jiang,Bo Yang,Neil S Harris,et al.Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots[J].Journal of Experimental Botany,2007,58:3591-3607
[6] 刘云岩,苏震,董江丽,等.截型苜蓿生物数据分析平台的构建与应用[J].草地学报,2006,14(3):231-235
[7] Kanako Kawaura,Keiichi Mochida,Yukiko Yamazaki,et al.Transcriptome analysis of salinity stress responses in common wheat using a 22k oligo-DNA microarray[J].Funct Integr Genomics,2006,6:132-142
[8] Francisco Merchan,Laura de Lorenzo,Silvina Gonza lezRizzo,et al.Identification of regulatory pathways involved in thereacquisition of root growth after salt stress in Medicago truncatula[J].The Plant Journal,2007,51:1-17
[9] 刘爱荣,赵可夫.盐胁迫下盐芥渗透调节物质的积累及其渗透调节作用[J].植物生理与分子生物学学报,2005,31(4):389-395
[10] Motoaki Seki,Marl Narusaka,Junko Ishida,et al.Monitoring the expression profiles of 7000 Arabidopsis genes under drought,cold and high-salinity stresses using a full-length cDNA microarray[J].The Plant Journal,2002,31:279-292
[11] Vadim Volkov,Anna Amtmann.Thellungiella halophila salt-tolerant relative of Arabidopsis thaliana,has specific root ion-channel features supporting K+/Na+homeostasis under salinity stress[J].The Plant Journal,2006,48(3):342-353
[12] Teruaki Taji,Motoaki Seki,MasakazuSatou,et al.Comparatire genomics in salt tolerance between Arabidopsis and Arabidopsis-related halophyte salt cress using Arabidopsis microarray[J].Plant Physiology,2004,135(3):1697-1709
[13] 李彦,张英鹏,孙明,等.盐分胁迫对植物的影响及植物耐盐机理研究进展[J].植物生理科学,2008,24(1):258-261
[14] Rana Munns.Genes and salt tolerance:bring them together[J].New Phytologist,2005,167:645-663
[15] 单雷,赵双宜,夏光敏.植物耐盐相关基因及其耐盐机制的研究[J].分子植物育种,2006,4(1):15-22
[16] 王宁宁,张韧,王勇.细胞分裂素对大豆叶片衰老过程中蛋白激酶基因表达的影响[J].南开大学学报,1998,31(2):100-101
[17] 葛荣朝,赵宝存,陈桂平,等.小麦耐盐相关基因TaSTK的克隆[J].作物学报,2007,33(5):857-860
[18] Ryu H Y,Kim S Y,Park H M,et al.Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death[J].Biochemical and Biophysical Research Communications,2009,379(2):417-422
[19] Oh M H,Wang X,Kota U,et al.Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of brassinosteroid signaling in Arabidopsis[J].Proceedings of the National Academy of Sciences of the United States of America,2009,106(2):658-663
[20] 王波.植物耐盐研究进展[J].农业系统科学与综合研究,2007,23(2):212-216
[21] Moreno J I,Martín R,Castresana C.Arabidopsis SHMT1 a serine hydroxymethyltransfera pathway influences in the photorespiratory resistance to biotic and abiotic stress[J].ThePlant Journal,2005,41:451-463

盐胁迫条件下苦马豆幼苗根差异表达基因及功能注释

Differentially Expressed Genes of Swainsonine Roots under Salinity Stress and Its Annotation

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