外源NO对渗透胁迫下多年生黑麦草幼苗生长和生理特性的影响

doi:10.11733/j.issn.1007-0435.2011.04.015

›› 2011, Vol. 19 ›› Issue (4): 625-630.DOI: 10.11733/j.issn.1007-0435.2011.04.015

外源NO对渗透胁迫下多年生黑麦草幼苗生长和生理特性的影响

高景慧¹, 母养秀¹, 张越利¹, 葛志颂¹, 闫文¹, 邵怀梅²

1. 西北农林科技大学动物科技学院, 陕西杨凌712100;
2. 陕西省洛川县永乡乡人民政府, 727400

收稿日期:2010-09-08 修回日期:2011-04-09 出版日期:2011-08-15 发布日期:2011-08-15
作者简介:高景慧(1970- ),女,辽宁沈阳人,讲师,博士,主要从事牧草育种及生理生态的研究,E-mail:gaojinghui@nwsuaf.edu.cn
基金资助:
教育部重点项目(105167);博士科研启动经费(2010BSJJ003);国家科技支撑项目(2011BAD17B05)资助

Effects of Exogenous Nitric Oxide on the Growth and Physiological Characteristics of Perennial Ryegrass Seedlings under Osmotic Stress

GAO Jing-hui¹, MU Yang-xiu¹, ZHANG Yue-li¹, GE Zhi-song¹, YAN Wen¹, SHAO Huai-mei²

1. College of Animal and Technology, Northwest A & F University, Yangling, Shaanxi Province 712100, China;
2. Yongxiang Township of the People’s Government, Luochuan County, Shaanxi Province 727400, China

Received:2010-09-08 Revised:2011-04-09 Online:2011-08-15 Published:2011-08-15

摘要/Abstract

摘要： 一氧化氮(Nitric oxide,NO)作为生物体广布的一种生理活性物质,可调节植物一系列生理生化反应,增强其对逆境的抵抗能力;为此,以15%PEG-6000模拟渗透胁迫,研究外源NO对多年生黑麦草(Lolium perenne L.)幼苗生长和生理特性的影响。结果表明:15%PEG-60000渗透胁迫下,0.1 mmol·L^-1NO供体硝普钠(Sodium nitroprus-side,SNP)可显著减轻对幼苗生长的抑制;促进脯氨酸(Pro)的累积,延缓幼苗叶片的水分散失(P<0.05);可诱导超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性升高,减缓丙二醛(MDA)的积累,显著缓解因渗透胁迫诱导的细胞膜脂过氧化过程(P<0.05),增强其抗渗透胁迫能力,且随着胁迫程度的加剧效果越佳。0.5 mmol·L^-1SNP处理对黑麦草幼苗生长影响不显著,促进保护酶活性及缓解氧化损伤的作用较0.1 mmol·L^-1SNP处理显著减弱。外源NO诱导植物水分及抗氧化酶系活性的变化,是植物在渗透胁迫下维持正常生长的重要抗逆生理机制。

关键词: 多年生黑麦草, 一氧化氮(NO), 渗透胁迫, 幼苗生长, 生理特性

Abstract: Nitric oxide (NO) as a physiological active substance is widespread in organisms and can regulate a series of physiology and biochemical reactions of plant to enhance adversity resistance.The effects of sodium nitropprus-side (SNP,an exogenous nitric oxide donor) on growth and physiological characteristics were investigated in perennial ryegrass (Lolium perenne L.) seedling under osmotic stress (15%PEG-6000).Results showed that under osmotic stress (15%PEG-6000),0.1 mmol·L^-1 SNP significantly alleviated growth inhibition of perennial ryegrass seedling,accelerated accumulation of proline (Pro) and delayed water dissipation.The activity of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT) were enhanced and the content of malondialdehyde (MDA) were reduced significantly in perennial ryegrass seedling leaves under osmotic stress.The damage of perennial ryegrass seedlings caused by the osmotic stress of 15%PEG-6000 was significantly reduced by 0.1 mmol·L^-1 SNP.A higher concentration of SNP (0.5 mmol·L^-1) had less effect on growth and alleviating oxidative damage in perennial ryegrass seedlings.Exogenous nitric oxide induces changes in water content of leaves and antioxidant enzyme systems in plants,which is an important anti-adversity mechanism of plant physiology response to maintain natural growth under osmotic stress.

Key words: Perennial ryegrass (Lolium perenne L.), Nitric oxide (NO), Osmotic stress, Seeding growth, Physiological characteristics

中图分类号:

Q945.78

高景慧, 母养秀, 张越利, 葛志颂, 闫文, 邵怀梅. 外源NO对渗透胁迫下多年生黑麦草幼苗生长和生理特性的影响[J]. , 2011, 19(4): 625-630.

GAO Jing-hui, MU Yang-xiu, ZHANG Yue-li, GE Zhi-song, YAN Wen, SHAO Huai-mei. Effects of Exogenous Nitric Oxide on the Growth and Physiological Characteristics of Perennial Ryegrass Seedlings under Osmotic Stress[J]. , 2011, 19(4): 625-630.

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外源NO对渗透胁迫下多年生黑麦草幼苗生长和生理特性的影响

Effects of Exogenous Nitric Oxide on the Growth and Physiological Characteristics of Perennial Ryegrass Seedlings under Osmotic Stress

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