CO2浓度倍增及干旱胁迫对紫花苜蓿光合生理特性的协同影响

doi:10.11733/j.issn.1007-0435.2014.01.014

›› 2014, Vol. 22 ›› Issue (1): 85-93.DOI: 10.11733/j.issn.1007-0435.2014.01.014

CO₂浓度倍增及干旱胁迫对紫花苜蓿光合生理特性的协同影响

樊良新^1,2, 刘国彬^1,3, 薛萐^1,3, 杨婷¹, 张昌胜¹

1. 西北农林科技大学资源环境学院, 陕西杨凌 712100;
2. 河南理工大学测绘与国土信息工程学院, 河南焦作 454003;
3. 中国科学院水利部水土保持研究所, 陕西杨凌 712100

收稿日期:2013-05-16 修回日期:2013-07-15 出版日期:2014-02-15 发布日期:2014-01-28
通讯作者: 薛萐，E-mail：xuesha100@163.com
作者简介:樊良新（1979-），男，安徽六安人，博士研究生，主要从事生态规划管理研究，E-mail：fanliangxin@163.com
基金资助:
西北农林科技大学基本科研业务费专项（ZD2013021）；中国科学院战略性先导科技专项（XDA05060300）；陕西省科学技术研究发展计划项目（2011KJXX63）资助

Synergistic Effects of Doubled CO₂ Concentration and Drought Stress on the Photosynthetic Characteristics of Medicago sativa

FAN Liang-xin^1,2, LIU Guo-bin^1,3, XUE Sha^1,3, YANG Ting¹, ZHANG Chang-sheng¹

1. College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. School of Surveying and Land Information Engineering, Henan Polytechnic University, Jiaozuo, Henan Province 454003, China;
3. Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resources, Yangling, Shaanxi Province 712100, China

Received:2013-05-16 Revised:2013-07-15 Online:2014-02-15 Published:2014-01-28

摘要/Abstract

摘要： 为探讨CO₂浓度倍增和干旱胁迫对紫花苜蓿（Medicago sativa）生理作用的协同效应，采用人工气候室和控水试验模拟CO₂浓度倍增和干旱胁迫，分析了植物光合和抗逆生理特征的变化规律。结果表明：干旱胁迫与CO₂浓度倍增对苜蓿光响应参数影响显著，二者之间具有一定的交互作用，在干旱胁迫下，CO₂浓度倍增减缓了苜蓿叶片气孔导度（G_s）、蒸滕速率（T_r）和光饱和点（LSP）的降低幅度，而对最大净光合速率（P_max）、胞间CO₂浓度（C_i）和表观量子效率（AQE）没有减缓作用。CO₂浓度倍增下的水分利用效率（WUE）高于正常CO₂浓度，而与水分含量关系不大。干旱胁迫与CO₂浓度倍增对苜蓿光合色素、丙二醛（MDA）和脯氨酸（Pro）含量影响显著，干旱胁迫在一定程度上降低了叶绿素a（chl a）、叶绿素b（chl b）的含量和叶绿素总量，显著增加了叶片MDA和Pro含量，CO₂浓度倍增有增加这些光合色素含量、降低MDA和Pro的趋势；但2个因素之间没有显著交互作用。干旱胁迫和CO₂浓度倍增对苜蓿叶绿素荧光参数影响不一，干旱胁迫和CO₂浓度倍增对叶绿素荧光参数具有较强的交互作用，CO₂浓度倍增对植物体光合电子传递过程中的保护作用在植物受到干旱胁迫时更加明显。因此，在干旱、半干旱地区，干旱胁迫对苜蓿光合生理功能产生不利的影响，而CO₂浓度倍增对苜蓿的生长具有一定的施肥效应，当2个因素共同发生时，CO₂浓度倍增可以减缓水分胁迫对苜蓿的伤害，增强其抗旱能力，提高水分利用效率，缓解干旱胁迫的负面效应。

关键词: 紫花苜蓿, 光合生理特性, CO₂浓度倍增, 干旱胁迫, 协同效应

Abstract: In order to study the photo-physiological characteristics of Medicago sativa in the loess hilly-gully region under different water stress and CO₂ concentration, the photosynthetic and physiological characteristics of plant were analyzed with different scenarios using artificial climate chamber and water control simulation. Results showed that the photo-physiological characteristics of alfalfa were significantly affected by drought stress and doubled CO₂ concentration, and there were synergistic effects between them. Under drought stress, the doubled CO₂ concentration improved G_s, T_r and LSP, but had no effect on P_max, C_i and AQE, as compared with those under ambient CO₂ concentration. The water use efficiency (WUE) under double CO₂ concentration was higher than under normal CO₂ concentration, but had no significant correlation with moisture content. Drought stress and doubled CO₂ concentration had significantly effects on photosynthetic pigment, malondialdehyde (MDA) and proline (Pro). Drought stress, to a certain extent, decreased the chlorophyll a (chl a), chlorophyll b (chl b) and the total chlorophyll contents, while increased significantly the MDA and Pro contents of plant. Doubled CO₂ concentration decreased the photosynthetic pigment contents of plant, and improved the MDA and Pro contents of plant. However, there were no significant synergistic effects between them. Drought stress and double CO₂ concentration had different effects on the fluorescence parameters of chlorophyll, and there was a strong synergistic effect between them. Under drought stress, doubled CO₂ concentration had more obvious protection on the photosynthetic electron transport in the plants. Therefore, the above results indicated that in arid and semi-arid area, doubled CO₂ concentration had definite compensation effects on the photosynthetic physiological functions of alfalfa induced by drought stress, and enhanced drought resistance capacity, improved water use efficiency and alleviated the negative effects of drought stress on alfalfa.

Key words: Medicago sativa, Photo-physiological characteristics, Doubled CO₂ concentration, Drought stress, Synergic effect

中图分类号:

樊良新, 刘国彬, 薛萐, 杨婷, 张昌胜. CO₂浓度倍增及干旱胁迫对紫花苜蓿光合生理特性的协同影响[J]. , 2014, 22(1): 85-93.

FAN Liang-xin, LIU Guo-bin, XUE Sha, YANG Ting, ZHANG Chang-sheng. Synergistic Effects of Doubled CO₂ Concentration and Drought Stress on the Photosynthetic Characteristics of Medicago sativa[J]. , 2014, 22(1): 85-93.

参考文献

[1] Smith T M, Karl T R, Reynolds R W. Climate modeling. How accurate are climate simulations?[J]. Science, 2002, 296(5567):483-484
[2] DeLucia E H, Hamilton J G, Naidu S L. Net primary production of a forest ecosystem with experimental CO₂ enrichment[J]. Science, 1999, 284(5417):1177-1179
[3] Philips O L, Halhi Y, Higuchi N. Changes in carbon balance of tropical forests: Evidence from long-term plots[J]. Science, 1998, 282(5388):439-442
[4] IPCC. Climate Change 2001: Synthesis report: The third assessment report of the intergovernmental panel on climate change[M]. Cambridge: Cambridge University Press, 2001:35-38
[5] 尚宗波, 高琼. 中国水分状况对全球气候变化的敏感性分析[J]. 生态学报, 2001, 21(4):528-537
[6] King J S, Thomas R B, Strain B R. Growth and carbon accumulation in root systems of Pinus taeda and Pinus ponderosa seedlings as affected by varying CO₂ temperature, and nitrogen[J]. Tree Physiology, 1996, 16(7):635-642
[7] Murray M B, Smith R I, Friend A, et al. Effect of elevated[CO₂] and varying nutrient application rates on physiology and biomass accumulation of Sitka spruce (Picea sitchensis)[J]. Tree Physiology, 2000, 20(7):421-434
[8] 王精明, 李永华, 黄胜琴, 等. CO₂浓度升高对红掌光合速率与生长发育的影响[J]. 园艺学报, 2005, 32(2):335-338
[9] 孙加伟, 赵天宏, 付宇, 等. CO₂浓度升高对玉米叶片光合生理特征的影响[J]. 玉米科学, 2009, 17(2):81-85
[10] Vasseur P C. Long-term CO₂ enrichment of a pasture community: Species richness, dominance, and succession[J]. Ecology, 1997, 78(13):666-677
[11] Joel G, ChaPin F S, Chiariello N R, et al. Species specific responses of plant communities to altered carbon and nutrient availability[J]. Global Change Biology, 2001, 7(4):435-450
[12] Rodiyati A, Arisoesilaningsih E, Isagi Y, et al. Responses of Cyperus brevifolius (Rottb.) Hassk. and Cyperus kyllingia Endl. to varying soil water availability[J]. Environmental and Experimental Botany, 2005, 53(3):259-269
[13] 尹丽, 胡庭兴, 刘永安, 等. 干旱胁迫对不同施氮水平麻疯树幼苗光合特性及生长的影响[J]. 应用生态学报, 2010, 21(3):569-576
[14] 王美玉, 赵天宏, 张巍巍, 等. CO₂ 浓度升高与温度、干旱相互作用对植物生理生态过程的影响[J]. 干旱地区农业研究, 2007, 25(2):99-103
[15] Yu J J, Chen L H, Xu M, et al. Effects of elevated CO₂ on physiological responses of tall fescue to elevated temperature, drought stress, and the combined stresses[J]. Crop Science, 2012, 52(4):1848-1858
[16] Markelz R J, Strellner R S, Leakey A. Impairment of C₄ photosynthesis by drought is exacerbated by limiting nitrogen and ameliorated by elevated CO₂in maize[J]. Journal of Experimental Botany, 2011, 62(9):3235-3246
[17] Vu J C V, Allen L H. Growth at elevated CO₂ delays the adverse effects of drought stress on leaf photosynthesis of the C₄ sugarcane[J]. Journal of Plant Physiology, 2009, 166(2):107-116
[18] Wall G W, Garciab R L, Wechsungc F, et al. Elevated atmospheric CO₂ and drought effects on leaf gas exchange properties of barley[J]. Agriculture, Ecosystems and Environment, 2011, 144(1):390-404
[19] 高素华, 郭建平, 周广胜. 高CO₂浓度下羊草对土壤干旱胁迫的响应[J]. 中国生态农业学报, 2002, 10(4):31-33
[20] Parry M, Rosenzweig C, Iglesias A, et al. Effects of climate change on global food production under SRES emissions and socio-economic scenarios[J]. Global Environmental Change, 2004, 14(1):53-67
[21] 李清明, 刘彬彬, 邹志荣. CO₂浓度倍增对干旱胁迫下黄瓜幼苗光合特性的影响[J]. 中国农业科学, 2011, 44(5):963-971
[22] 李清明, 刘彬彬, 艾希珍. CO₂ 浓度倍增对干旱胁迫下黄瓜幼苗膜脂过氧化及抗氧化系统的影响[J]. 生态学报, 2010, 30(22):6063-6071
[23] Wullschleger S D, Tschaplinski T J, Norby R J. Plant water relations at elevated CO₂ implications for water limited environments[J]. Plant, Cell and Environment, 2002, 25(2):319-331
[24] Leakey A D B, Uribelarrea M, Ainsworth E A, et al. Photosynthesis, productivity and yield of maize are not affected by open air elevation of CO₂ concentration in the absence of drought[J]. Plant Physiology, 2006, 140(2):779-790
[25] Wechsung G, Wechsung F, Wall G W, et al. The effects of free air CO₂ enrichment and soil water availability on special and seasonal patterns of wheat root growth[J]. Global Change Biology, 1999, 5(5):519-529
[26] 王俊, 刘文兆, 钟良平, 等. 长期连续种植苜蓿草地地上部分生物量与土壤水分的空间差异性[J]. 草业学报, 2009, 18(4): 41-46
[27] Sartory D R, Grobbelaar J U. Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis[J]. Hydrobiologia, 1984, 114(3):177-187
[28] 张志安, 张美善, 尉荣海. 植物生理学实验指导[M]. 北京:中国农业技术出版社, 2002:4-7
[29] 邹琦, 孟庆伟, 高辉远, 等. 作物在非生物逆境下的光合作用[M]. 济南:山东科学技术版社, 2004:116-172
[30] 孙谷畴, 赵平, 曾小平, 等. 倍增CO₂分压对水稻和矶子草冠层光合潜力的影响[J]. 生态学杂志, 2003, 22(4):1-5
[31] 何平. 温室效应与植物光合作用: CO₂浓度升高对植物光合机理影响的分析[J]. 中南林学院学报, 2001, 21(1):1-4
[32] 李伏生, 康绍忠, 张富仓. CO₂浓度、氮和水分对春小麦光合、蒸散及水分利用效率的影响[J]. 应用生态学报, 2003, 14(3):387-393
[33] 白莉萍, 周广胜. 全球环境变化对农作物影响的研究进展[J]. 应用与环境生物学报, 2004, 10(3):394-397
[34] Qaderi M M, Kurepin L V, Reid D M. Growth and physiological responses of canola (Brassica napus) to three components of global climate change: Temperature, carbon dioxide and drought[J]. Physiologia Plantarum, 2006, 128(4):710-721
[35] Robredo A, Pérez-López U, Maza H S, et al. Elevated CO₂ alleviates the impact of drought on barley improving water status by lowering stomatal conductance and delaying its effects on photosynthesis[J]. Environmental and Experimental Botany, 2007, 59(3):252-263
[36] Lichtenthaler H K. Application of chlorophyll fluorescence in research stree physiology, hydrobiology and remote sensing[M]. Dordrecht: Kluwer Academic Publisher, 1991:253-258
[37] 王建程, 严昌荣, 卜玉山. 不同水分与养分水平对玉米叶绿素荧光特性的影响[J]. 中国农业气象, 2005, 26(12):95-98
[38] Hamerlynck E P, Huxman T E, Loik M E, et al. Effects of extreme high temperature, drought and elevated CO₂ on photosynthesis of the Mojave Desert evergreen shrub, Larrea tridentate[J]. Plant Ecology, 2000, 148(4):183-193
[39] Aranjuelo I, Perez P, Hernandez L, et al. The response of nodulated alfalfa to water supply, temperature and elevated CO₂: Photosynthetic down regulation[J]. Physiologia Plantarum, 2005, 123(3):348-358
[40] 王可玢, 许春辉, 赵福洪, 等. 水分胁迫对小麦旗叶某些体内叶绿素a荧光参数的影响[J].生物物理学报, 1997, 13(2):273-278
[41] 赵丽英, 邓西平, 山仑. 不同水分处理下冬小麦旗叶叶绿素荧光参数的变化研究[J]. 中国生态农业学报, 2007, 15(1):63-66
[42] 严美玲, 李向东, 林英杰, 等. 苗期干旱胁迫对不同抗旱花生品种生理特征、产量和品质的影响[J]. 作物学报, 2007, 33(1):113-119
[43] Liang J, Zhang J, Wong M H. Stomatal conductance in relation to xylem sap ABA concentration in two tropical trees, Acacia confusa and Litsea glutinosa[J]. Plant, Cell and Environment, 1996, 19(1):93-100
[44] Hanson A D, Neison C F, Pedersen A R, et al. Capacity for proline accumulation during water stress in barley and its implication for breeding for drought resistance[J]. Crop Science, 1979, 19(2):489-493
[45] Singh T N, Aspnall D, Paleg L G. Proline accumulation and varietal adaptability to drought in barley, a potential metabolic measure of drought resistance[J]. Nature New Biology, 1972, 236(67):188-190
[46] 刘娥娥, 宗会, 郭振飞, 等. 干旱、盐和低温胁迫对水稻幼苗脯氨酸含量的影响[J]. 热带亚热带植物学报, 2000, 8(3):235-238

CO₂浓度倍增及干旱胁迫对紫花苜蓿光合生理特性的协同影响

Synergistic Effects of Doubled CO₂ Concentration and Drought Stress on the Photosynthetic Characteristics of Medicago sativa

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王静, 刘晓静, 程甜甜, 童长春, 汪雪. 不同氮效率紫花苜蓿叶特征及其产量效应研究[J]. 草地学报, 2021, 29(9): 1941-1949.
[2]	孟宣辰, 马鹏程, 马杰, 段珍, 韩阳阳, 张吉宇. 黄土高原半干旱区紫花苜蓿覆膜垄沟和施肥效应研究[J]. 草地学报, 2021, 29(9): 2098-2106.
[3]	佟莉蓉, 倪顺刚, 任星远, 刘嘉欣, 闻静, 王娟, 宋雨. 褪黑素对干旱胁迫下达乌里胡枝子幼苗生长及叶片水分生理的影响[J]. 草地学报, 2021, 29(8): 1682-1688.
[4]	陈雷, 暴雪艳, 郭刚, 霍文婕, 李清宏, 许庆方, 王聪, 刘强. 单宁酸和乳酸菌对紫花苜蓿青贮品质和体外瘤胃发酵的影响[J]. 草地学报, 2021, 29(8): 1853-1858.
[5]	李春萍, 邱轶辉, 夏厚胤, 谭璐娜, 许环宇, 张瀚文, 高景慧. 紫花苜蓿多父本F₁代低蒸腾及产量性状的隶属函数分析[J]. 草地学报, 2021, 29(7): 1416-1422.
[6]	李天琦, 林志玲, 卢轩, 黄佳媛, 杨宁, 沃野, 高凯, 柳小妮, 王显国. 灌溉量对科尔沁沙地紫花苜蓿土壤速效养分分布及淋失的影响[J]. 草地学报, 2021, 29(7): 1454-1461.
[7]	李鹏珍, 赵得琴, 邓波, 杨军, 赵国华, 赵亮. 生物炭与干旱胁迫对接种紫花苜蓿光合效率及生长的影响[J]. 草地学报, 2021, 29(6): 1257-1264.
[8]	王文静, 麻冬梅, 赵丽娟, 马巧利. 2,4-表油菜素内酯对盐胁迫下紫花苜蓿生理指标及根系离子积累的影响[J]. 草地学报, 2021, 29(6): 1363-1368.
[9]	潘新怡, 史昆, 龚攀, 韦宝, 吴欣明, 王赞. 紫花苜蓿花青苷合成的转录组分析[J]. 草地学报, 2021, 29(5): 866-875.
[10]	黄荣, 姚博, 张玉娟, 贾倩英, 李向阳, 张宏, 张振粉. 成团泛菌CQ10脂多糖对紫花苜蓿种子萌发及苗期生长的影响[J]. 草地学报, 2021, 29(5): 965-971.
[11]	徐洪雨, 甄莉丽, 李钰莹, 董宽虎, 李向林. 低温干旱环境对紫花苜蓿根颈耐寒性的影响[J]. 草地学报, 2021, 29(4): 724-733.
[12]	李满有, 马忠仁, 王斌, 杨雨琦, 沈笑天, 曹立娟, 李小云, 兰剑. 宁夏引黄灌区2个苜蓿品种不同行比混播模式研究[J]. 草地学报, 2021, 29(4): 798-804.
[13]	侯红雁, 申晨, 霍文婕, 刘强, 张拴林, 王聪, 陈雷, 许庆方, 王永新, 王忠豪, 郭刚. 同型发酵乳杆菌对低水分紫花苜蓿青贮发酵品质及体外消化率的影响[J]. 草地学报, 2021, 29(4): 835-841.
[14]	陈德奎, 郭香, 郑明扬, 陈晓阳, 周玮, 张庆. 砂仁精油对紫花苜蓿青贮品质的影响[J]. 草地学报, 2021, 29(4): 855-860.
[15]	夏方山, 王聪聪, 李红玉, 刘曼, 郑川, 张耀丹, 范华芳. 硒引发对紫花苜蓿种子抗氧化性能的影响[J]. 草地学报, 2021, 29(3): 472-477.