草地学报

草地学报 ›› 2014, Vol. 22 ›› Issue (6): 1295-1300.DOI: 10.11733/j.issn.1007-0435.2014.06.023

• 研究论文 • 上一篇    下一篇

磁化水处理对苜蓿根瘤菌生长和结瘤固氮的影响

何媛1, 罗明1, 李卫军2, 曹晖1, 郭学良2   

  1. 1. 新疆农业大学农学院, 新疆 乌鲁木齐 830052;
    2. 新疆农业大学草业与环境科学学院, 新疆 乌鲁木齐 830052
  • 收稿日期:2014-02-11 修回日期:2014-04-09 出版日期:2014-12-15 发布日期:2014-12-01
  • 通讯作者: 罗明
  • 作者简介:何媛(1987-),女,新疆昌吉人,硕士研究生,研究方向为微生物生物技术,E-mail: 1452095510@qq.com
  • 基金资助:

    国家现代农业产业技术体系“牧草现代产业技术体系”(CARS35)资助

Effects of Magnetized Water on the Growth and Nitrogen Fixation of Rhizobial Strain on Alfalfa

HE Yuan1, LUO Ming1, LI Wei-jun2, CAO Hui1, GUO Xue-liang2   

  1. 1. Agronomy Faculty, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China;
    2. College of Pratacultural Science and Environmental, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China
  • Received:2014-02-11 Revised:2014-04-09 Online:2014-12-15 Published:2014-12-01

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摘要：

为明确磁化水对根瘤菌生长及结瘤固氮的作用,测定在2万Gs恒定磁场下处理的磁化水对苜蓿根瘤菌生长及其结瘤固氮的影响.结果表明:不同磁化次数的磁化水均能显著影响XGL026菌株生长,降低OD值,磁化水培养基中的活菌数为普通培养基的86.96%～65.72%,菌数降低幅度随磁化次数增加而增大,磁化3次以上的磁化水对活菌数的降低效应极为显著(P<0.01).在盆栽苜蓿上接种绿色荧光蛋白基因(GFP)标记菌株XGL026-GFP,采用磁化水灌溉对根瘤菌在根际土壤中的存活和定殖具有抑制作用,苜蓿单株结瘤数与普通水对照相比平均减少46.4%(P<0.01).磁化水处理的苜蓿地上、地下部分的固氮率分别降低21.06%和20.48%,固氮量分别降低26.64%和37.5%,植株干物质重分别减少27.62%和13.46%.表明磁化水处理对苜蓿根瘤菌的生长、定殖和固氮及植株干物质重均有抑制作用.

关键词: 根瘤菌, 磁化水, 生物效应, 固氮率

Abstract:

The effects of magnetized water treatment on the growth and nitrogen fixation of rhizobial strain on alfalfa under the constant magnetic field of 20000 Gs were investigated. The results showed that magnetization significantly affected the growth of XGL026 and reduced the OD value. The counts of viable bacteria in magnetized water culture media were the 86.96%~65.72% of ordinary culture media, and the counts of viable bacteria decreased with the times of magnetization increasing. The water magnetized more than three times significantly reduced the counts of viable bacteria (P<0.01). Pot alfalfa was inoculated by the marked bacteria strain XGL026-GFP. The irrigation of magnetized water had inhibitory effect on the colonization and survival of the rhizobial in alfalfa rhizosphere and reduced 46.4% nodule number compared to ordinary water (P<0.01). Both above- and below-ground of alfalfa were influenced by magnetized water treatment, the rates of N fixation decreased 21.06% and 20.48%, the amount of N fixation decreased by 26.64% and 37.5%, the dry biomasses were reduced by 27.62% and 13.46%, respectively. Thus the magnetized water treatment had effects on the growth, colonization and nitrogen fixation of rhizobial strain in alfalfa rhizosphere.

Key words: Rhizobia, Magnetized water, Biological effect, The proportion of N fixed

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