根瘤共生对紫花苜蓿抗寒性的影响

doi:10.11733/j.issn.1007-0435.2018.03.015

草地学报 ›› 2018, Vol. 26 ›› Issue (3): 631-638.DOI: 10.11733/j.issn.1007-0435.2018.03.015

根瘤共生对紫花苜蓿抗寒性的影响

刘雨诗¹, 耿金才², 宋江湖³, 沙煦旸¹, 呼天明¹, 杨培志¹

1. 西北农林科技大学动物科技学院草业科学系, 陕西杨凌 712100;
2. 陕西省草原工作站, 陕西西安 710016;
3. 陕西省林业科学研究院, 陕西西安 710082

收稿日期:2017-12-28 修回日期:2018-06-02 出版日期:2018-06-15 发布日期:2018-08-24
通讯作者: 呼天明, 杨培志
作者简介:刘雨诗(1991-),女,重庆渝中人,博士研究生,主要从事牧草逆境研究,E-mail:ys_liu@hotmail.com
基金资助:
国家自然科学基金（31571456）；国家牧草产业体系（CARS-34）资助

Effect of Root Nodules on Cold Resistance of Alfalfa (Medicago sativa)

LIU Yu-shi¹, GENG Jin-cai², SONG Jiang-hu³, SHA Xu-yang¹, HU Tian-ming¹, YANG Pei-zhi¹

1. Department of Grassland Science, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi Province 712100, China;
2. Shaanxi Grassland Workstation, Xi'an, Shaanxi Province 710016, China;
3. Shaanxi Forestry Institute of Sciences, Xi'an, Shaanxi Province 710082, China

Received:2017-12-28 Revised:2018-06-02 Online:2018-06-15 Published:2018-08-24

摘要/Abstract

摘要：

以保定紫花苜蓿（Medicago sativa L.‘Baoding’）幼苗为试验材料，分别进行接种根瘤菌处理并确认结瘤固氮（activated-nodules，AN）和不接种根瘤菌处理（non-nodules，NN）。将生长120 d的植株放在-6℃培养箱低温胁迫12 h。通过测定叶片电导率、丙二醛MDA、渗透势、过氧化氢H₂O₂、超氧阴离子自由基O₂^·-、还原性谷胱甘肽GSH、抗坏血酸VC、叶绿素a、叶绿素b、叶绿素总量等10个指标，研究根瘤共生对紫花苜蓿抗寒性的影响。结果表明：与不接种根瘤菌苜蓿相比，低温胁迫导致根瘤固氮苜蓿较轻的细胞膜损伤和氧化损伤，并且根瘤固氮苜蓿在低温胁迫中叶绿素破坏较不接种根瘤菌苜蓿缓慢，说明根瘤与紫花苜蓿共生可以提高苜蓿抗寒性。

关键词: 紫花苜蓿, 根瘤菌, 低温胁迫, 抗寒

Abstract:

Alfalfa (Medicago sativa L. ‘Baoding’) seedlings were exposed to be inoculated rhizobium (activated-nodules, AN) and non-inoculated rhizobia (non-nodules, NN), respectively. The 120-day seedlings were subjected to cold stress (-6℃) for 12 hours. The physiological changes were analyzed including leaf electrical conductivity, methane dicarboxylic aldehyde (MDA), leaf osmotic potential, hydrogen peroxide (H₂O₂), superoxide anion radical (O₂^·-), reduced glutathione (GSH), Vitamin C (VC), chlorophyll a, b and total chlorophyll content. Results showed that compared with alfalfa without nodules, alfalfa with activated nodules had lower cell membrane and oxidative damage when they were exposed to cold stress. Moreover, chlorophyll in AN decreased more slowly under cold stress than that in NN. These results indicate that rhizobia and alfalfa symbiotic interaction could improve alfalfa cold tolerance.

Key words: Alfalfa, Rhizobium, Low temperature stress, Cold tolerance

中图分类号:

S551+.7

刘雨诗, 耿金才, 宋江湖, 沙煦旸, 呼天明, 杨培志. 根瘤共生对紫花苜蓿抗寒性的影响[J]. 草地学报, 2018, 26(3): 631-638.

LIU Yu-shi, GENG Jin-cai, SONG Jiang-hu, SHA Xu-yang, HU Tian-ming, YANG Pei-zhi. Effect of Root Nodules on Cold Resistance of Alfalfa (Medicago sativa)[J]. Acta Agrestia Sinica, 2018, 26(3): 631-638.

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根瘤共生对紫花苜蓿抗寒性的影响

Effect of Root Nodules on Cold Resistance of Alfalfa (Medicago sativa)

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