假俭草抗寒性状与低温胁迫响应EST分子标记的关联分析

doi:10.11733/j.issn.1007-0435.2014.06.024

草地学报 ›› 2014, Vol. 22 ›› Issue (6): 1301-1307.DOI: 10.11733/j.issn.1007-0435.2014.06.024

假俭草抗寒性状与低温胁迫响应EST分子标记的关联分析

王鹏良^1,2, 王海燕¹, 刘建秀³, 王秀娥¹

1. 南京农业大学作物遗传与种质创新国家重点实验室细胞遗传研究所, 江苏南京 210095;
2. 广西壮族自治区林业科学研究院, 广西南宁 530002;
3. 江苏省中国科学院植物研究所, 江苏南京 210014

收稿日期:2014-01-14 修回日期:2014-04-20 出版日期:2014-12-15 发布日期:2014-12-01
通讯作者: 王秀娥
作者简介:王鹏良(1978-),男,浙江新昌人,博士,研究方向为植物分子细胞遗传学,E-mail: pengliang_wang@163.com
基金资助:
国家自然科学基金(30670200);广西自然科学基金(2012GXNSFBA053037);广西林科院基金项目(林科200807号)资助

Association Mapping of Cold Tolerance with Molecular Markers from Cold Responsive ESTs in Eremochloa ophiuroides

WANG Peng-liang^1,2, WANG Hai-yan¹, LIU Jian-xiu³, WANG Xiu-e¹

1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
2. Guangxi Forestry Research Institute, Nanning, Guangxi 530001, China;
3. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu Province 210014, China

Received:2014-01-14 Revised:2014-04-20 Online:2014-12-15 Published:2014-12-01

摘要/Abstract

摘要：

为深入研究抗寒分子机理以及为分子标记辅助育种奠定基础,以具有代表性的96份种源为材料,利用假俭草(Eremochloa ophiuroides)抗寒品种TifBlair低温诱导后所得的EST及其近缘种高粱NCBI中低温胁迫响应的EST开发分子标记,对假俭草抗寒指标LT₅₀观测值和EST分子标记位点进行关联分析.结果表明:开发的17对引物在假俭草群体共扫描到46个多态遗传位点,其中CINAU114-900,CINAU115-1500和CINAU116-400位点与抗寒指标LT₅₀有显著关联,其表型变异的解释率分别为5.10%, 4.04%和4.59%;其LT₅₀表型效应分别为0.2518℃,-0.3121℃和0.2449℃.CINAU114-900和CINAU116-400位点提高假俭草对低温的敏感性,而CINAU115-1500位点可提高其抗寒性.

关键词: 假俭草, 抗寒性状, EST分子标记, 关联分析

Abstract:

The genotyping data on the representative 96 accessions in China were obtained using the primers of the ESTs (Expressed Sequence Tags) derived from sorghum in NCBI and the SSH library induced by cold stress in centipedegrass. Population structure was firstly analyzed, then association mapping between EST molecular marker loci and the index of cold tolerance LT₅₀ was performed by using the TASSEL GLM program. The results indicated that 46 polymorphic genetic loci were amplified from the 17 primer pairs. Of them, CINAU114-900, CINAU115-1500, CINAU116-400 were significantly associated with the index of cold tolerance, LT₅₀. Their explained portion of phenotypic variation were 5.10%,4.04% and 4.59%, respectively. Phenotypic effects of the three LT₅₀ were 0.2518℃,-0.3121℃ and 0.2449℃, respectively. CINAU114-900 and CINAU116-400 decreased the cold tolerance, whereas CINAU115-1500 increased the cold tolerance.

Key words: Eremochloa ophiuroides, Cold tolerance, EST molecular marker, Association mapping

中图分类号:

S432.4

王鹏良, 王海燕, 刘建秀, 王秀娥. 假俭草抗寒性状与低温胁迫响应EST分子标记的关联分析[J]. 草地学报, 2014, 22(6): 1301-1307.

WANG Peng-liang, WANG Hai-yan, LIU Jian-xiu, WANG Xiu-e. Association Mapping of Cold Tolerance with Molecular Markers from Cold Responsive ESTs in Eremochloa ophiuroides[J]. Acta Agrestia Sinica, 2014, 22(6): 1301-1307.

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假俭草抗寒性状与低温胁迫响应EST分子标记的关联分析

Association Mapping of Cold Tolerance with Molecular Markers from Cold Responsive ESTs in Eremochloa ophiuroides

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