草地学报

草地学报 ›› 2019, Vol. 27 ›› Issue (4): 841-847.DOI: 10.11733/j.issn.1007-0435.2019.04.007

• 研究论文 • 上一篇    下一篇

野生甘蒙锦鸡儿的组织培养研究

赵培霞, 王雷   

  1. 内蒙古农业大学林学院, 内蒙古 呼和浩特 010019
  • 收稿日期:2019-03-13 修回日期:2019-07-01 出版日期:2019-08-15 发布日期:2019-09-26
  • 通讯作者: 王雷
  • 作者简介:赵培霞(1994-),女,内蒙古呼和浩特人,硕士,主要从事园林植物与人居环境研究,E-mail:1640742077@qq.com
  • 基金资助:
    内蒙古自治区自然科学基金项目（2015MS0346）；内蒙古农业大学双一流（203/170005）资助

Study on Tissue Culture of Wild Caragana Opulens Kom.

ZHAO Pei-xia, WANG Lei   

  1. College of Forestry, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China
  • Received:2019-03-13 Revised:2019-07-01 Online:2019-08-15 Published:2019-09-26

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摘要： 本试验采用甘蒙锦鸡儿（Caragana opulens Kom.）成熟种子为材料，利用组织培养的方法进行成熟胚的诱导，当胚成苗后进行增殖和生根培养，建立了一套野生甘蒙锦鸡儿的组培体系，为保护甘蒙锦鸡儿野生资源及其引种到城市园林建设中提供一定的借鉴作用。结果表明：试验前将种子用蒸馏水浸泡一晚效果较好；当灭菌时间为15 min时污染率最小，为37.61%；MS+6-BA 0.5 mg·L-1+NAA 0.05 mg·L-1培养基为最适宜成熟胚离体诱导的培养基；MS+6-BA 0.5mg·L-1+NAA 0.05 mg·L-1培养基为最适宜增殖的培养基；再生苗移植到1/2 MS+IAA 0.1 mg·L-1培养基上，植株健壮，生根率为63.16%；将生根培养的甘蒙锦鸡儿幼苗移栽到草炭土：珍珠岩：蛭石=6：3：1的混合基质中，成活率为90%。

关键词: 甘蒙锦鸡儿, 成熟种子, 方差分析, 组织培养, 植株再生

Abstract: In this experiment,mature seeds of Caragana opulens Kom. were used as materials to induce mature embryos by tissue culture. After emergence of seedlings,shoot multiplication culture and rooting culture were made. In this test,a set of tissue culture system of wild C. opulens. was established to provide some reference for protecting wild resources of C. opulens. and its introduction to urban garden construction. The test results showed that it was better to soak the seeds in distilled water for one night before the experiment. The minimum contamination rate was 37.61% when the sterilization time was 15 minutes. MS + 6-BA 0.5 mg·L-1 + NAA 0.05 mg·L-1 medium is the most suitable medium for in vitro induction of mature embryos. MS+6-BA 0.5 mg·L-1+NAA 0.05 mg·L-1 medium was the most suitable medium for proliferation. When the regenerated seedlings were transplanted on 1/2 MS + IAA 0.1 mg·L-1 medium,the plant was robust,and the rooting rate was 63.16%. When the rooting seedlings of C. opulens. were transplanted into a mixed growth medium,in which the ratio of peat:perlite:vermiculite is 6:3:1,the survival rate was 90%.

Key words: Caragana opulens Kom., Mature seeds, Variance analysis, Tissue culture, Plant regeneration

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