不同添加剂对高水分全株玉米青贮饲料发酵品质和细菌群落的影响

doi:10.11733/j.issn.1007-0435.2021.04.027

草地学报 ›› 2021, Vol. 29 ›› Issue (4): 842-847.DOI: 10.11733/j.issn.1007-0435.2021.04.027

不同添加剂对高水分全株玉米青贮饲料发酵品质和细菌群落的影响

陆永祥^1,3*, 严显明², 周朝相², 朱会先², 苟文龙³, 游明鸿³, 李达旭³, 陈仕勇¹, 李平^3*, 白史且^1,3*

1.西南民族大学, 四川成都 610041;
2.会东县黑山羊研究所, 四川会东 615200;
3.四川省草原科学研究院, 四川成都 611731

收稿日期:2020-10-31 修回日期:2020-12-14 发布日期:2021-06-02
通讯作者: * E-mail:lpyzm@sina.cn;baishiqie@126.com
作者简介:陆永祥(1996-),男,辽宁丹东人,硕士研究生,主要从事牧草生产与加工利用研究,E-mail:2489362105@qq.com
基金资助:
国家牧草产业技术体系阿坝综合试验站(CARS-34)资助

Effects of Different Additives on Fermentation Quality and Bacterial Community of High-moisture Whole-plant Corn Silage

LU Yong-xiang^1,3, YAN Xian-ming², ZHOU Zhao-xiang², ZHU Hui-xian², GOU Wen-long³, YOU Ming-hong³, LI Da-xu³, CHEN Shi-yong¹, LI Ping^3*, BAI Shi-qie^1,3*

1. Southwest University for Minzu, Chengdu, Sichuan Province 610041, China;
2. Huidong County Black Goat Research Institute, Huidong, Sichuan Province 615200, China;
3. Sichuan Academy of Grassland Science, Chengdu, Sichuan Province 611731, China

Received:2020-10-31 Revised:2020-12-14 Published:2021-06-02
Contact: * E-mail:lpyzm@sina.cn;baishiqie@126.com

摘要/Abstract

摘要： 为探讨不同添加剂对高水分全株玉米(Zea mays L.)青贮饲料发酵品质和细菌群落的影响,本试验以乳熟期的全株玉米为原料,设置对照、添加苯甲酸钠和植物乳杆菌3个处理,青贮60 d后,测定其营养成分、发酵指标和细菌群落组成。结果表明:植物乳杆菌和苯甲酸钠均能使高水分全株玉米青贮饲料的细菌群落发生一定变化,并以乳杆菌属细菌为优势菌群(>70%);2种添加剂均未降低高水分全株玉米青贮饲料的细菌多样性;与对照相比,用植物乳杆菌处理高水分全株玉米青贮后,其氨态氮含量呈显著增加的趋势(P<0.05),用苯甲酸钠处理的高水分全株玉米青贮后,pH值升高(P<0.05),有机酸含量降低。因此,2种添加剂对高水分全株玉米青贮饲料发酵品质的改善效果不佳。

关键词: 添加剂, 全株玉米, 青贮, 发酵品质, 细菌群落

Abstract: To explore the effects of lactobacillus plantarum and Sodium benzoate on the fermentation quality and bacterial community of whole corn silage with high moisture,the whole-plant corn was taken as ensiling material under three treatments in our study,including control,Lactobacillus plantarum and Sodium benzoate. And then,its chemical composition,fermentation profile and bacterial community were measured after 60 days silage. The results showed that Lactobacillus plantarum and Sodium benzoate had a certain effect on changing the bacterial community composition,and Lactobacillus was the dominant genus (>70%),but these two additives did not reduced the bacterial diversity of the high-moisture whole-plant corn silage. In contrast to control,the ammonia nitrogen content was significantly increased (P<0.05) under Lactobacillus plantarum,while pH was increased (P<0.05) and the organic acid content was decreased under Sodium benzoate.Therefore,the two additives have poor effects on improving the fermentation quality of high-moisture whole-plant corn silage.

Key words: Additive, Whole-plant corn, Silage, Fermentation quality, Bacterial community

中图分类号:

S816.5+3

陆永祥, 严显明, 周朝相, 朱会先, 苟文龙, 游明鸿, 李达旭, 陈仕勇, 李平, 白史且. 不同添加剂对高水分全株玉米青贮饲料发酵品质和细菌群落的影响[J]. 草地学报, 2021, 29(4): 842-847.

LU Yong-xiang, YAN Xian-ming, ZHOU Zhao-xiang, ZHU Hui-xian, GOU Wen-long, YOU Ming-hong, LI Da-xu, CHEN Shi-yong, LI Ping, BAI Shi-qie. Effects of Different Additives on Fermentation Quality and Bacterial Community of High-moisture Whole-plant Corn Silage[J]. Acta Agrestia Sinica, 2021, 29(4): 842-847.

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参考文献

[1] 祁宏伟,田子玉,姜怀志,等. 不同收获时间全株玉米青贮饲料在牛瘤胃内干物质降解率的研究[J].吉林农业大学学报,2001(4):97-99,110
[2] 李鸿,蒋志成,冯定明,等. 玉米青贮、黄贮研究进展及展望[J]. 南方农业,2021,15(1):79-81
[3] 李福勇. 我国发展全株玉米青贮的意义及其影响因素[J]. 山东畜牧兽医,2014,35(3):80-81
[4] 王飞,董祥,朱欣,等. 乳酸菌添加剂对高水分全株玉米青贮品质和饲用效益的影响[J]. 黑龙江畜牧兽医,2019(18):118-122,126
[5] 王强,王超丽,师燕霞. 影响青贮饲料品质的因素[J]. 石河子科技,2018,238(2):7-9
[6] 袁仕改,陈超,张明均,等. 添加剂对温湿生境青贮玉米发酵品质及有氧稳定性的影响[J].草地学报,2018,26(4):942-947
[7] 李旭业,徐艳霞,董扬,等. 青贮添加剂种类及其对青贮饲料品质的影响[J]. 现代畜牧科技,2016(1):32-33
[8] 王青兰,谢展,张志飞. 有机酸盐在青贮发酵中的应用研究进展[J]. 草学,2020(6):13-18,29
[9] Liu B,Huan H,Gu H,et al. Dynamics of a microbial community during ensiling and upon aerobic exposure in lactic acid bacteria inoculation-treated and untreated barley silages[J]. Bioresource Technology,2019,273:212-219
[10] 陶莲,刁其玉. 青贮发酵对玉米秸秆品质及菌群构成的影响[J]. 动物营养学报,2016,28(1):198-207
[11] 陆永祥,成启明,陈良寅,等.不同海拔的燕麦青贮饲料发酵品质和细菌群落特征[J].草地学报,2020,28(2):350-357
[12] Chen L Y,Bai S Q,You M H,et al. Effect of a low temperature tolerant lactic acid bacteria inoculant on the fermentation quality and bacterial community of oat round bale silage[J]. Animal Feed ence and Technology,2020,269:3-4
[13] 陆永祥,赵嫚,陈良寅,等. 布氏乳杆菌和甲酸对青藏高原不同物候期燕麦青贮饲料发酵品质和细菌群落的影响[J]. 草地学报,2020,28(6):251-258
[14] Murphy R P. A method for the extraction of plant samples and the determination of total soluble carbohydrates[J]. Journal of the Science of Food and Agriculture,1958,9:714-717
[15] Broderick G A,Kang J H. Automated Simultaneous Determination of Ammonia and Total Amino Acids in Ruminal Fluid and In Vitro Media 1[J]. Journal of Dairy Science,1980,63(1):64-75
[16] Cai Y,Benno Y,Ogawa M,et al. Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage[J]. Journal of dairy science,1999,82(3):520-526
[17] Li P,Zhang Y,Gou W,et al. Silage fermentation and bacterial community of bur clover,annual ryegrass and their mixtures prepared with microbial inoculant and chemical additive[J]. Animal Feed Science and Technology,2019,247:285-293
[18] 郭金桂,吴哲,玉柱. 不同比例有机酸及其盐类添加剂对燕麦青贮品质及有氧稳定性的影响[C] //中国草学会.2017中国草学会年会论文集. 2017:6
[19] Kung L,Shaver R D,Grant R J,et al. Silage review:Interpretation of chemical,microbial,and organoleptic components of silages[J]. Journal of Dairy ence,2018,101(5):4020-4033
[20] 苗芳. 同/异质型乳酸菌对玉米青贮品质及有氧稳定性的影响[D]. 石河子:石河子大学,2017:1-5
[21] 唐庆凤,杨承剑,彭开屏,等. 添加植物乳杆菌对桑枝叶与玉米秸秆混合青贮发酵品质的影响[J]. 饲料工业,2018,39(19):42-47
[22] 王飞,董祥,朱欣,等. 乳酸菌添加剂对高水分全株玉米青贮品质和饲用效益的影响[J]. 黑龙江畜牧兽医,2019(18):118-122,126
[23] Mu L,Xie Z,Hu L X,et al. Cellulase interacts with Lactobacillus plantarum to affect chemical composition,bacterial communities,and aerobic stability in mixed silage of high-moisture amaranth and rice straw[J]. Bioresource Technology,2020,315:123772
[24] Yang L,Yuan X,Li J,et al. Dynamics of microbial community and fermentation quality during ensiling of sterile and nonsterile alfalfa with or without Lactobacillus plantarum inoculant[J]. Bioresource Technology,2019,275:280-287
[25] 付彤. 玉米青贮饲料中优势乳酸菌的筛选与青贮发酵调控技术研究[D]. 郑州:河南农业大学,2014:3
[26] Ni K,Wang F,Zhu B,et al. Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage[J]. Bioresource Technology,2017,238:706-715
[27] Gautam N,Sharma N. A study on characterization of new bacteriocin produced from a novel strain of Lactobacillus spicheri G2 isolated from Gundruk- a fermented vegetable product of North East India:A novel bacteriocin production from Lactobacillus spicheri G2[J]. Journal of Food Science & Technology,2015,52(9):5808-16
[28] 陈鹏飞. 玉米和玉米青贮中优良乳酸菌的分离鉴定及应用研究[D]. 雅安:四川农业大学,2012:4-5
[29] Muck R E,McAllister T A,Contreras-Govea F E,et al. Silage review:Recent advances and future uses of silage additives [J]. Journal of Dairy Science,2018,101(5):3980-4000
[30] 徐生阳,闵旭东,云颖,等. 短乳杆菌及不同品种对全株玉米青贮品质和CNCPS组分的影响[J]. 中国畜牧杂志,2018,54(8):96-100

不同添加剂对高水分全株玉米青贮饲料发酵品质和细菌群落的影响

Effects of Different Additives on Fermentation Quality and Bacterial Community of High-moisture Whole-plant Corn Silage

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