紫花苜蓿根际土壤细菌群落对腐熟牛粪响应

doi:10.11733/j.issn.1007-0435.2022.03.012

草地学报 ›› 2022, Vol. 30 ›› Issue (3): 603-611.DOI: 10.11733/j.issn.1007-0435.2022.03.012

• 研究论文 • 上一篇

紫花苜蓿根际土壤细菌群落对腐熟牛粪响应

王芳¹, 李伟^2,3, 刘鑫¹, 李文辰¹, 赵金波^2,3, 张智慧^2,3, 杨曌^2,3

1. 齐齐哈尔大学生命科学与农林学院, 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江齐齐哈尔 161006;
2. 黑龙江省农业科学院畜牧兽医分院, 黑龙江齐齐哈尔 161005;
3. 黑龙江省牧草育种与种质资源利用工程技术研究中心, 黑龙江齐齐哈尔 161005

收稿日期:2021-05-06 修回日期:2021-06-21 发布日期:2022-03-30
通讯作者: 王芳,E-mail:wangfangnd@hotmail.com
作者简介:王芳(1981-),女,山东即墨人,副教授,主要从事微生物多样性研究,E-mail:wangfangnd@hotmail.com
基金资助:
财政部和农业农村部：国家现代农业产业技术体系CARS-34；黑龙江省教育厅基本科研业务费科研项目(135309361)；黑龙江省农业科学院“农业科技创新跨越工程”专项奶牛提质增效技术研究与示范(HNK2019CX15)资助

Bacteria Communities of Medicago sativa Rhizosphere Soil in Response to Composted Cow Manure

WANG Fang¹, LI Wei^2,3, LIU Xin¹, LI Wen-chen¹, ZHAO Jin-bo^2,3, ZHANG Zhi-hui^2,3, YANG Zhao^2,3

1. College of Life Sciences and Agroforestry, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China;
2. Heilongjiang Academy of Agricultural Sciences Livestock Veterinary Branch, Qiqiha, Heilongjiang Province 161005, China;
3. Heilongjiang Engineering Technology Research Center of Forage Breeding and Germplasm Resources Utilization, Qiqihar, Heilongjiang Province 161005, China

Received:2021-05-06 Revised:2021-06-21 Published:2022-03-30

摘要/Abstract

摘要： 为了探讨腐熟牛粪施用量对作物根际土壤细菌丰度、多样性及群落结构的影响，本研究以盆栽紫花苜蓿(Medicago sativa L.)为试验材料，分别添加0%，3%，6%，9%的腐熟牛粪，在添加30d，60d，90d后进行取样，利用Illumina-Miseq高通量测序平台对土壤细菌16S rRNA V3-V4区进行测序。结果表明，放线菌门(Actinobacteria)和变形菌门(Proteobacteria)是所有供试样品的优势菌门。添加牛粪降低了土壤细菌多样性和丰富度(P<0.05)，增加了土壤厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和变形菌门的丰度，增加芽孢杆菌属(Bacillus)、马杜拉放线菌属(Actinomadura)的丰度(P<0.05)。聚类分析显示，全土与全粪两个样品含有的一些细菌丰度相似。添加牛粪对一些优势菌丰度产生显著影响，其土壤中溶杆菌属(Lysobacter)、芽孢杆菌属、糖单胞菌属(Saccharomonospora)、直丝菌属(Planifilum)、纤维菌属(Cellvibrio)、Steroidobacter、德沃斯氏菌属(Devosia)的丰度显著高于未添加组、全土和全粪(P<0.05)。不同牛粪添加比例均降低土壤细菌OTU数量，但添加比例至6%牛粪的土壤细菌OTU数目随着苜蓿生长持续增加，添加至9%的土壤细菌OTU数目持续降低。

关键词: 紫花苜蓿, 腐熟牛粪, 土壤细菌群落, 高通量测序

Abstract: In order to determine the effects of the application amount of composted cow manure on the community diversity and structure of crop rhizosphere soil bacteria,this study sampled potted alfalfa (Medicago sativa L.) soils supplemented with 0%,3%,6%,and 9% composted cow manure for 30 d,60 d,and 90 d respectively,sequenced V3-V4 region of soil bacterial 16S rRNA using Illumina-Miseq high-throughput. The results showed that Actinobacteria and Proteobacteria were the dominant phyla in all tested samples. Addition of composted cow manure decreased the diversity and richness of soil bacteria (P<0.05),significantly increased the abundance of Firmicutes,Bacteroidetes,and Proteobacteria,as well as increased the abundance of Bacillus and Actinomadura (P<0.05). Cluster analysis showed that the abundance of bacterial genus abundance was similar between the whole soil and the whole manure samples. The addition of composted cow manure significantly affected the abundance of some dominant bacteria. The abundance of genera Lysobacter,Bacillus,Saccharomonospora,Planifilum,Cellvibrio,Steroidobacter,Devosia in the mixed samples were significantly higher than those in the non-fertilization group,the whole soil as well as the whole manure (P<0.05). The number of soil bacterial OTU decreased with supplemental proportions,but the number of soil bacterial OTU increased with the growth of alfalfa at 6% cow manure application rate and decreased continuously at 9% manure application rate.

Key words: Medicago sativa, Composted cow manure, Soil bacterial community, High-throughput sequencing

中图分类号:

S154

王芳, 李伟, 刘鑫, 李文辰, 赵金波, 张智慧, 杨曌. 紫花苜蓿根际土壤细菌群落对腐熟牛粪响应[J]. 草地学报, 2022, 30(3): 603-611.

WANG Fang, LI Wei, LIU Xin, LI Wen-chen, ZHAO Jin-bo, ZHANG Zhi-hui, YANG Zhao. Bacteria Communities of Medicago sativa Rhizosphere Soil in Response to Composted Cow Manure[J]. Acta Agrestia Sinica, 2022, 30(3): 603-611.

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紫花苜蓿根际土壤细菌群落对腐熟牛粪响应

Bacteria Communities of Medicago sativa Rhizosphere Soil in Response to Composted Cow Manure

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