草地学报

草地学报 ›› 2022, Vol. 30 ›› Issue (9): 2399-2406.DOI: 10.11733/j.issn.1007-0435.2022.09.020

• 研究论文 • 上一篇    下一篇

ABA和GA3对山韭种子萌发、生理特性及内源激素含量的影响

胡国富, 李韦瑶, 肖汇川, 崔国文, 宋雪, 韦银珠, 苏滢淼, 秦立刚   

  1. 东北农业大学动物科学技术学院, 黑龙江 哈尔滨 150030
  • 收稿日期:2022-04-02 修回日期:2022-05-01 出版日期:2022-09-15 发布日期:2022-09-30
  • 通讯作者: 秦立刚,E-mail:qinligang@neau.edu.cn
  • 作者简介:胡国富(1974-),男,黑龙江哈尔滨人,教授,博士,主要从事草地植物资源利用研究,E-mail:guofuh2003@163.com
  • 基金资助:
    国家自然科学基金（31702160）；哈尔滨市科技创新人才项目（2017RAQXJ007）资助

Effects of ABA and GA3 on Seed Germination, Physiological Characteristics and Endogenous Hormone Contents of Allium senescens L.

HU Guo-fu, LI Wei-yao, XIAO Hui-chuan, CUI Guo-wen, SONG Xue, WEI Yin-zhu, SU Ying-miao, QIN Li-gang   

  1. College of Animal Science, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, China
  • Received:2022-04-02 Revised:2022-05-01 Online:2022-09-15 Published:2022-09-30

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摘要： 本研究通过不同浓度的脱落酸（Abscisicacid，ABA）和赤霉素（Gibberellins，GA3）浸种，研究其对山韭（Allium senescens L.）种子萌发特征的影响及促进萌发的生理变化规律。结果表明：100 mg·L-1 ABA和100 mg·L-1 GA3处理显著提高了山韭种子的发芽率、发芽势、胚芽长、胚根长和胚芽胚根的鲜重（P<0.05）。与施加蒸馏水处理相比，ABA和GA3处理均显著提高了可溶性蛋白含量、脂肪酶和蛋白酶活性（P<0.05）；100 mg·L-1 GA3处理显著提高了山韭胚芽乙烯（Ethrel，ETH）含量和ABA含量（P<0.05），100 mg·L-1 ABA处理显著增加了山韭胚芽GA3的含量而显著降低了ABA含量（P<0.05）。主成分分析和相关性分析表明：山韭种子发芽率、发芽指数和活力指数与可溶性蛋白、可溶性糖含量以及酶活性之间显著正相关。100 mg·L-1 ABA和100 mg·L-1 GA3对山韭种子的萌发及生长均具有显著的促进作用，ABA处理通过提高山韭胚芽内源GA3含量促进种子萌发，GA3处理通过提高山韭胚芽内源ETH含量促进种子萌发。

关键词: 山韭, 种子萌发, 植物生长调节剂, 主成分分析

Abstract: In this study, different concentrations of abscisic acid (ABA) and gibberellin (GA3) were used to soak seeds to study their effects on the germination characteristics of Allium senescens L. seeds and the physiological changes in promoting germination. The results showed that 100 mg·L-1 ABA and 100 mg·L-1 GA3 treatments significantly increased the germination potential, germ length, radicle length, and fresh weight of germ radicle (P < 0.05). Compared with distilled water, both ABA and GA3 treatments significantly increased the soluble protein content, lipase, and protease activity (P < 0.05);GA3 treatment significantly increased the ETH content and ABA content in the germ of A. senescens (P < 0.05), and 100 mg·L-1 ABA treatment significantly increased the content of GA3 and decreased the content of ABA significantly (P < 0.05). Principal component analysis and correlation analysis showed that the germination rate, germination index, and vigor index of A. senescens seeds were significantly positively correlated with soluble protein, soluble sugar, and enzyme activity. The germination and growth of A. senescens seeds were significantly promoted under the treatments of 100 mg·L-1 ABA and 100 mg·L-1 GA3. ABA treatment promoted seed germination by increasing the content of GA3 in the germs, and GA3 treatment promoted the germination of seeds by increasing the content of ETH.

Key words: Allium senescens L., Seed germination, Plant growth regulator, Principal component analysis

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