丛枝菌根真菌对垂穗披碱草的生长发育和防御性酶活的影响

doi:10.11733/j.issn.1007-0435.2022.12.007

草地学报 ›› 2022, Vol. 30 ›› Issue (12): 3225-3232.DOI: 10.11733/j.issn.1007-0435.2022.12.007

• 研究论文 • 上一篇

丛枝菌根真菌对垂穗披碱草的生长发育和防御性酶活的影响

耿依仪¹, 于露¹, 张万通¹, 周华坤², 邓艳芳³, 康海燕⁴, 冶文哥⁵, 邵新庆¹

1. 中国农业大学草业科学与技术学院, 北京 100193;
2. 中国科学院西北高原生物研究所青海省寒区恢复生态学重点实验室, 青海西宁 810001;
3. 祁连山国家公园青海服务保障中心, 青海西宁 810001;
4. 青海省海北州祁连县峨堡镇畜牧兽医站, 青海海北 810400;
5. 青海省海北州祁连县野牛沟林场边麻管护站, 青海海北 810499

收稿日期:2022-07-01 修回日期:2022-08-19 发布日期:2023-01-04
通讯作者: 邵新庆,E-mail:shaoxinqing@163.com
作者简介:耿依仪(2001-),女,汉族,河南许昌人,本科生,主要从事草地恢复生态学研究,E-mail:11peterpan@sina.com;于露(1995-),女,汉族,吉林松原人,博士研究生,主要从事草地恢复生态学研究,E-mail:yulu5890@163.com
基金资助:
青海省科技厅创新平台建设专项(2020-ZJ-T07)资助

Effects of Arbusculae Mycorrhizal Fungi on Growth Development and Defense Enzymes of Elymus nutans Griseb

GENG Yi-yi¹, YU Lu¹, ZHANG Wan-tong¹, ZHOU Hua-kun², DENG Yan-fang³, KANG Hai-yan⁴, YE Wen-ge⁵, SHAO Xin-qing¹

1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
2. Qinghai Provincial Key Laboratory of the Cold Regions Restoration Ecology, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810001, China;
3. Qinghai forestry and grassland administration, Xining, Qinghai Province 810002, China;
4. Animal Husbandry and Veterinary Station of EbaoTown, Qilian County, Haibei Prefecture, Qinghai Province 810400, China;
5. Bianma management station of Yeniugou Forest Farm, Qilian County, Haibei Prefecture, Qinghai Province 810499, China

Received:2022-07-01 Revised:2022-08-19 Published:2023-01-04

摘要/Abstract

摘要： 为研究丛枝菌根真菌(Arbusculae mycorrhizal fungi，AMF)在植物抵御虫害过程中的作用，本研究以垂穗披碱草(Elymus nutans Griseb.)为供体植物，老芒麦(Elymus sibiricus Linn.)为受体植物，以摩西管柄囊霉(Funneliformis mosseae)作为接种菌种，共设置5个处理，即：存在菌丝，但无根系连接(A)、无菌丝和根系连接(B)、无根系但有菌丝连接，且在虫害处理前旋转(C)、既有菌丝又有根系连接(D)、无接菌但存在根系连接(E)，测定了菌根侵染率、地上地下生物量、碳氮含量以及防御酶活性等指标。结果表明：AM真菌显著提高了受体植物地下生物量(P<0.05)；虫害发生后，AM真菌诱导了供受体植物体内的防御酶活性显著升高(P<0.05)。本试验证明了AMF可以促进宿主植物的生长发育，菌丝网络则为主要防御化学信号的传递途径。

关键词: 垂穗披碱草, 丛枝菌根真菌, 菌丝网络, 抗虫防御

Abstract: Elymus nutans Griseb. was used as the donor plant and Elymus sibiricus Linn. was used as the recipient plants to study the role of arbusculae mycorrhizal fungi (AMF) in plants resisting insect pests in this study. Funneliformis mosseae was selected as the inoculum. A total of 5 treatments，including the presence of hyphae but no root connection (A),sterile filaments and Root system connection (B),no root system but with mycelium connection and rotation before pest treatment (C),both mycelium and root system connection (D),and no inoculation but root system connection (E),were set up to measure AMF colonization,aboveground and belowground biomass,carbon and nitrogen content and defense enzymes. The results showed that， AM fungi significantly increased underground biomass of receiver plants(P<0.05);AM fungi induced a significant increase in defense enzyme activities in donor and receiver plants after the occurrence of insect pests(P<0.05). This experiment indicated that AMF not only promoted development growth of host plants,but also its mycelial network could act as a pathway for the transmission of defensive chemical signals between plants.

Key words: Elymus nutans Griseb, Arbuscular mycorrhizal fungi, Common mycelial network, Insect resistance

中图分类号:

Q939.5

耿依仪, 于露, 张万通, 周华坤, 邓艳芳, 康海燕, 冶文哥, 邵新庆. 丛枝菌根真菌对垂穗披碱草的生长发育和防御性酶活的影响[J]. 草地学报, 2022, 30(12): 3225-3232.

GENG Yi-yi, YU Lu, ZHANG Wan-tong, ZHOU Hua-kun, DENG Yan-fang, KANG Hai-yan, YE Wen-ge, SHAO Xin-qing. Effects of Arbusculae Mycorrhizal Fungi on Growth Development and Defense Enzymes of Elymus nutans Griseb[J]. Acta Agrestia Sinica, 2022, 30(12): 3225-3232.

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参考文献

[1] 邵梓桐,秦彧. 高原鼠兔干扰对高寒草地碳循环的影响研究进展[J]. 草地学报,2022,30(5):1086-1094
[2] SHI W,WANG X,YIN Y,et al. Dynamics of Aboveground Natural Enemies of Grasshoppers,and Biodiversity after Application of Paranosema locustae in Rangeland[J]. Insects,2019,10(8):224
[3] 才仁旦周. 青藏高原鼠虫害防治工作建议[J]. 中国畜牧兽医文摘,2018,34(5):39
[4] 洪军,杜桂林,贠旭疆,等. 近10年来我国草原虫害生物防控综合配套技术的研究与推广进展[J].草业学报,2014,23(5):303-311
[5] LEE B,MUNEER S,AVICE J,et al. Mycorrhizal Colonisation and P-supplement Effects on N uptake and N Assimilation in Perennial Ryegrass Under Well-watered and Drought-stressed Conditions[J]. Mycorrhiza,2012,22(7):525-534
[6] 李洋,严俊鑫,陈晓玲. AM真菌对植物虫害防治作用研究进展[J]. 农业生物技术学报,2019,27(9):1692-1702
[7] 张伟珍，古丽君，段廷玉. AM真菌提高植物抗逆性的机制[J]. 草业科学,2018,35(3):491-507
[8] SONG Y Y,WANG M,ZENG R,et al. Priming and Filtering of Antiherbivore Defences among Nicotiana attenuata Plants connected by Mycorrhizal Networks[J]. Plant,Cell and Environment,2019,42(11):2945-2961
[9] NISHIDA T,KATAYAMA N,IZUMI N,et al. Arbuscular Mycorrhizal Fungi Species-specifically Affect induced Plant Responses to a Spider Mite[J]. Population Ecology,2010,52(4):507-515
[10] 武帅. 丛枝菌根真菌定殖对银中杨生长及抗虫性的影响[D]. 哈尔滨:东北林业大学,2021:1-2
[11] FORMENTI L,RASMANN S. Mycorrhizal Fungi Enhance Resistance to Herbivores in Tomato Plants with Reduced Jasmonic Acid Production[J]. Agronomy (Basel),2019,9(3):131
[12] SONG Y Y,YE M,LI C,et al. Hijacking Common Mycorrhizal Networks for Herbivore-induced Defence Signal Transfer between Tomato Plants[J]. Scientific Reports,2015,4(1):3915
[13] SONG Y Y,YE M,LI C Y,et al. Priming of Anti-Herbivore Defense in Tomato by Arbuscular Mycorrhizal Fungus and Involvement of the Jasmonate Pathway[J]. Journal of Chemical Ecology,2013,39(7):1036-1044
[14] 徐雅梅,褚希彤,吴叶,等.接种丛枝菌根真菌对低温胁迫下垂穗披碱草影响的研究[J].草地学报,2016,24(5):1009-1015
[15] 孙永芳,付娟娟,褚希彤,等.丛枝菌根真菌对垂穗披碱草吸收不同氮源效率的影响[J].草地学报,2015,23(2):294-301
[16] 张海娟,芦光新,范月君,等. AM真菌对高寒草地2种禾本科牧草的接种效果研究[J]. 草地学报,2022,30(7):1684-1691
[17] 董高峰,武新宁,周淑丽,等. 青藏高原东北部河盆区线性工程沿线生态环境建设[J]. 青海环境,2009,19(2):53-57
[18] 张永超,魏小星,梁国玲,等.老芒麦衰老过程形态特征变化规律及对养分添加的响应[J].草业学报,2022,31(6):101-111
[19] KOVÁČIK J,GRU'Z J,BAČKOR M,et al. Salicylic Acid-induced Changes to Growth and Phenolic Metabolism in Matricaria chamomilla plants[J]. Plant Cell Reports,2009,28(1):135-143
[20] LI J,YANG C,LIU X,et al. Inconsistent Stoichiometry Response of Grasses and Forbs to Nitrogen and Water Additions in an Alpine Meadow of the Qinghai-Tibet Plateau[J]. Agriculture,Ecosystems and Environment,2019(279):178-186
[21] 冯远娇,陈卓娜,王建武,等. Bt玉米丛枝菌根真菌侵染率与养分含量的变化研究[J]. 中国生态农业学报,2010,18(3):486-491
[22] 刘月华,钟梦莹,武瑞鑫,等.AM真菌介导垂穗披碱草抗虫作用研究[J].草地学报,2016,24(3):604-609
[23] 王茜,王强,王晓娟,等. 丛枝菌根网络的生态学功能研究进展[J]. 应用生态学报,2015,26(7):2192-2202
[24] 刘欢. 不同丛枝菌根真菌对四种植物生长特性影响[D]. 兰州：甘肃农业大学,2016:1-2
[25] JOHNSON D,LEAKE J R,OSTLE N,et al. In Situ¹³CO₂ pulse-labelling of Upland Grassland Demonstrates a Rapid Pathway of Carbon Flux from Arbuscular Mycorrhizal Mycelia to the Soil[J]. New Phytologist,2002,153(2):327-334
[26] 任海燕,田磊,朱毅,等. 氮水添加改变内蒙古典型草原两种优势植物的氮吸收偏好[J]. 科学通报,2022,67(13):1459-1468
[27] GOVINDARAJULU M,PFEFFER P E,JIN H,et al. Nitrogen Transfer in the Arbuscular Mycorrhizal Symbiosis[J]. Nature,2005,435(7043):819-823
[28] 李洋,严俊鑫,陈晓玲. AM真菌对植物虫害防治作用研究进展[J]. 农业生物技术学报,2019,27(9):1692-1702
[29] WANG J,SONG Y Y,HU L,et al. Plant Anti-herbivore Defense Priming:Concept,Mechanisms and Application[J]. Chinese Journal of Applied Ecology,2018,29(6):2068-2078
[30] 廉法卓,林熠斌,胡林,等. 菌根菌丝网络介导的番茄植株间机械损伤信号的传递[J]. 福建农林大学学报(自然科学版),2019,48(1):9-15
[31] 刘福,尉敬涛,王宇宏,等. 丛枝菌根真菌(AMF)对棉花抗病防御酶系活性影响的研究[J].山西科技,2018,33(1):29-33
[32] 王彬,张金政,刘新,等. 丛枝菌根真菌诱导植物信号物质研究进展[J]. 微生物学通报,2010,37(2):263-268
[33] 谢丽君,宋圆圆,曾任森,等. 丛枝菌根菌丝桥介导的番茄植株根系间抗病信号的传递[J]. 应用生态学报,2012,23(5):1145-1152
[34] 江昌俊,余有本. 苯丙氨酸解氨酶的研究进展(综述)[J]. 安徽农业大学学报,2001(4):425-430
[35] SONG Y Y,ZENG R S,XU J F,et al. Interplant Communication of Tomato Plants through Underground Common Mycorrhizal Networks[J]. PloS One,2010,5(10):e13324

丛枝菌根真菌对垂穗披碱草的生长发育和防御性酶活的影响

Effects of Arbusculae Mycorrhizal Fungi on Growth Development and Defense Enzymes of Elymus nutans Griseb

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