细叶小檗生理、光合特性及叶表微形态对干旱胁迫的响应

doi:10.11733/j.issn.1007-0435.2023.05.013

草地学报 ›› 2023, Vol. 31 ›› Issue (5): 1386-1392.DOI: 10.11733/j.issn.1007-0435.2023.05.013

细叶小檗生理、光合特性及叶表微形态对干旱胁迫的响应

黄荣雁¹, 孟维珊³, 朱琨¹, 刘殿辉¹, 赵竹婷¹, 路程伟¹, 孙正轩¹, 唐宽宇¹, 周佳奇¹, 程瑜¹

1. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006;
2. 东北林业大学林学院, 黑龙江哈尔滨 150040;
3. 黑龙江省农业科学院畜牧兽医分院, 黑龙江齐齐哈尔 161006

收稿日期:2022-11-23 修回日期:2023-01-06 出版日期:2023-05-15 发布日期:2023-05-31
通讯作者: 黄荣雁,E-mail:hry_ok@163.com
作者简介:黄荣雁(1982-)，女，汉族，黑龙江齐齐哈尔人，副教授，博士研究生，主要从事森林培育学和林木病理学研究，E-mail:hry_ok@163.com
基金资助:
黑龙江省省属高校基本科研业务费项目(135509220)；中央财政林业科技推广示范资金项目(黑[2022]TG14号)；黑龙江省自然科学基金联合引导项目(LH2021C098)资助

Responses of Physiological,Photosynthetic Characteristics and Leaf Surface Micromorphology of Berberis poiretii to Drought Stress

HUANG Rong-yan¹, MENG Wei-shan³, ZHU Kun¹, LIU Dian-hui¹, ZHAO Zhu-ting¹, LU Cheng-wei¹, SUN Zheng-xuan¹, TANG Kuan-yu¹, ZHOU Jia-qi¹, CHENG Yu¹

1. College of Life Science, Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China;
2. Northeast Forestry University, Harbin, Heilongjiang Province 150040, China;
3. Animal Husbandry and Veterinary Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang Province 161006, China

Received:2022-11-23 Revised:2023-01-06 Online:2023-05-15 Published:2023-05-31

摘要/Abstract

摘要： 为探究细叶小檗(Berberis poiretii Schneid.)抗干旱能力,本研究采用盆栽控水的方法,对细叶小檗幼苗进行干旱胁迫处理,测定轻度、中度、重度干旱胁迫下细叶小檗幼苗生理、光合及叶表微形态的变化。结果表明:随着干旱胁迫程度的加剧,细叶小檗叶片相对含水量下降,丙二醛含量、脯氨酸含量逐渐升高,超氧化物歧化酶、过氧化物酶活性在中度干旱时达到最大值;净光合速率、气孔导度、蒸腾速率和胞间CO₂浓度均随胁迫的加剧呈递减趋势,而水分利用效率在重度干旱时大幅度提高;叶表蜡质覆盖面和气孔密度随胁迫的加剧呈增大趋势,而气孔大小和开度呈减小趋势。试验表明细叶小檗具有一定的抗旱能力,适合在中度干旱地区栽培,本研究为细叶小檗的开发利用提供参考依据。

关键词: 细叶小檗, 干旱胁迫, 生理特性, 光合特性, 叶表微形态

Abstract: In order to study the drought resistance of Berberis poiretii, the experiment of water control by pot culture was used to treat the seedlings of B. poiretii under drought stress,the changes of physiological characters,photosynthesis and leaf surface micromorphology of B. poiretii seedlings under mild,moderate and severe drought stress were investigated. The results showed that the relative water content (RWC) of B. poiretii leaves decreased,the content of malondialdehyde (MDA) and proline (Pro) increased with the intensification of drought stress,the contents of Superoxide dismutase (SOD)and Peroxidase (POD) reached the maximum in moderate drought,the net photosynthetic rate (P_n),stomatal conductance (G_s),transpiration rate (T_r) and intercellular CO₂ concentration (C_i) decreased with the intensification of drought stress,while the water use efficiency (WUE) increased greatly in the severe drought,the wax coverage and stomatal density of leaf surface increased with that intensification,while the stomatal size and opening degrees decreased. The results showed that B. poiretii had a certain drought-resistant ability and was suitable for cultivation in moderate arid area.

Key words: Berberis poiretii, Drought stress, Physiological characteristics, Photosynthetic characteristics, Leaf epidermis micromorphology

中图分类号:

Q945.78

黄荣雁, 孟维珊, 朱琨, 刘殿辉, 赵竹婷, 路程伟, 孙正轩, 唐宽宇, 周佳奇, 程瑜. 细叶小檗生理、光合特性及叶表微形态对干旱胁迫的响应[J]. 草地学报, 2023, 31(5): 1386-1392.

HUANG Rong-yan, MENG Wei-shan, ZHU Kun, LIU Dian-hui, ZHAO Zhu-ting, LU Cheng-wei, SUN Zheng-xuan, TANG Kuan-yu, ZHOU Jia-qi, CHENG Yu. Responses of Physiological,Photosynthetic Characteristics and Leaf Surface Micromorphology of Berberis poiretii to Drought Stress[J]. Acta Agrestia Sinica, 2023, 31(5): 1386-1392.

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细叶小檗生理、光合特性及叶表微形态对干旱胁迫的响应

Responses of Physiological,Photosynthetic Characteristics and Leaf Surface Micromorphology of Berberis poiretii to Drought Stress

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