草地早熟禾蛋白激酶CIPK24基因克隆及非生物胁迫响应分析

doi:10.11733/j.issn.1007-0435.2023.06.011

草地学报 ›› 2023, Vol. 31 ›› Issue (6): 1693-1701.DOI: 10.11733/j.issn.1007-0435.2023.06.011

草地早熟禾蛋白激酶CIPK24基因克隆及非生物胁迫响应分析

陈阳^1,2, 尤学¹, 刘文轩¹, 高岩松¹, 赵清峰¹, 熊毅^1,2, 金一锋^1,2

1. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006;
2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江齐齐哈尔 161006

收稿日期:2022-11-28 修回日期:2023-03-05 出版日期:2023-06-15 发布日期:2023-06-30
通讯作者: 金一锋,E-mail:Jinyifeng8368215@163.com
作者简介:陈阳(1986-)，女，黑龙江齐齐哈尔人，副教授，博士研究生，主要从事草坪草逆境研究，E-mail：Chenyang8368215@126.com
基金资助:
国家自然科学基金(31501785)；黑龙江省自然科学基金项目(QC2017026)；黑龙江省普通高等学校青年创新人才培养计划项目(UNPYSCT-2018102)；齐齐哈尔大学教育科学研究项目(GJQTZX20210 26)资助

Cloning of Protein Kinase CIPK24 Gene from Poa pratensis L. and Its Response to Abiotic Stresses

CHEN Yang^1,2, YOU Xue¹, LIU Wen-xuan¹, GAO Yan-song¹, ZHAO Qing-feng¹, XIONG Yi^1,2, JIN Yi-feng^1,2

1. College of Life Science and Agro-Forestry, Qiqihar Univesity, Qiqihar, Heilongjiang Province 161006, China;
2. Heilongjiang Province Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas, Qiqihar, Heilongjiang Province 161006, China

Received:2022-11-28 Revised:2023-03-05 Online:2023-06-15 Published:2023-06-30

摘要/Abstract

摘要： CIPK蛋白家族是Ca²⁺介导的植物信号通路中关键蛋白家族，当植物受到逆境胁迫时，CBL-CIPK网络可调节离子运输进而适应逆境环境，其中CIPK24作用显著。为探究禾本科草坪草CIPK家族基因的作用，本研究以草地早熟禾(Poa pratensis L.)为试验材料，采用RT-PCR技术对草地早熟禾CIPK24基因进行克隆，并应用生物信息学及qRT-PCR技术，对非生物胁迫条件下该基因的表达方式及组织特异性进行研究。研究结果显示，草地早熟禾CIPK24包含典型的结构域CIPK_C，其属于AMPKA_Clike superfamily，与黑麦草(Lolium perenne L.)，大麦(Hordeum vulgare L.)，二粒小麦(Triticum turgidum L.)CIPK24同源性高达94.20%，90.62%，88.62%。草地早熟禾CIPK24基因的表达水平存在组织特异性，叶＞茎＞根。CIPK24基因积极响应干旱、氮素、磷素、盐胁迫，其中，无氮、低氮、无磷、高盐环境显著促进其表达。本研究为丰富草地早熟禾CIPK家族在非生物胁迫下的调节机制提供了理论基础。

关键词: 草地早熟禾, 蛋白激酶, CIPK24, 非生物胁迫, 表达分析

Abstract: The CBL-interacting protein kinase (CIPK) family is a key protein family in the Ca²⁺-mediated signaling pathway in plants. Under stresses,plants can adapt to the environmental stresses by regulating the ion transport by the CBL-CIPK network,of which CIPK24 takes a significant role. To explore the role of the CIPK gene family in Poaceae turfgrasses,Poa pratensis L. was used as the experimental material in this study. The CIPK24 gene of P. pratensis L. was cloned using RT-PCR,and its expression pattern and tissue specificity under abiotic stresses were investigated by bioinformatics and qRT-PCR. The results showed the CIPK24 from Poa pratensis L. contained a typical structural domain CIPK_C and belonged to the AMPKA_Clike superfamily,with the homology of 94.20%,90.62% and 88.62% of the CIPK24 from Lolium perenne L.,Hordeum vulgare L. and Triticum turgidum L.,respectively. The expression level of the CIPK24 gene in P. pratensis L. was tissue-specific:leaves > stems > roots. The CIPK24 gene in P. pratensis took active responses to the drought,nitrogen,phosphorus and salt stresses. Nitrogen-free,low-nitrogen,phosphorus-free and high-salt environments significantly promoted its expression. This study may provide a theoretical basis for the regulatory mechanism of the CIPK family in Poa pratensis L. under abiotic stresses.

Key words: Kentucky bluegrass, Protein Kinase, CIPK24, Abiotic stress, Expression analysis

中图分类号:

Q344+.13

陈阳, 尤学, 刘文轩, 高岩松, 赵清峰, 熊毅, 金一锋. 草地早熟禾蛋白激酶CIPK24基因克隆及非生物胁迫响应分析[J]. 草地学报, 2023, 31(6): 1693-1701.

CHEN Yang, YOU Xue, LIU Wen-xuan, GAO Yan-song, ZHAO Qing-feng, XIONG Yi, JIN Yi-feng. Cloning of Protein Kinase CIPK24 Gene from Poa pratensis L. and Its Response to Abiotic Stresses[J]. Acta Agrestia Sinica, 2023, 31(6): 1693-1701.

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草地早熟禾蛋白激酶CIPK24基因克隆及非生物胁迫响应分析

Cloning of Protein Kinase CIPK24 Gene from Poa pratensis L. and Its Response to Abiotic Stresses

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