黄河上游区斑块化退化高寒草甸土壤线虫群落分布特征

doi:10.11733/j.issn.1007-0435.2023.11.005

草地学报 ›› 2023, Vol. 31 ›› Issue (11): 3258-3270.DOI: 10.11733/j.issn.1007-0435.2023.11.005

黄河上游区斑块化退化高寒草甸土壤线虫群落分布特征

陈国榕^1,2, 李希来¹, 李成一¹, 杨鹏年¹, 佘延娣¹, 吴勇¹

1. 青海大学农牧学院, 青海西宁 810016;
2. 重庆文理学院化学与环境工程学院, 重庆永川 402160

收稿日期:2023-05-08 修回日期:2023-08-17 出版日期:2023-11-15 发布日期:2023-12-01
通讯作者: 李希来,E-mail:xilai-li@163.com
作者简介:陈国榕(1992-),女,汉族,宁夏石嘴山人,博士研究生,主要从事土壤线虫及高寒草地生态修复方面的研究,E-mail:1045143259@qq.com
基金资助:
国家自然科学联合基金项目(U21A20191);青海省自然科学基金创新团队项目(2020-ZJ-904);高等学校学科创新引智计划项目(D18013);重庆市永川区科技计划项目(Ycstc,2020nb0302);重庆文理学院塔尖计划项目(P2019HH07)资助

Characteristics of Soil Nematode Community in the Different Degraded Patch of Alpine Meadow in the upper Yellow River Region

CHEN Guo-rong^1,2, LI Xi-lai¹, LI Cheng-yi¹, YANG Peng-nian¹, SHE Yan-di¹, WU Yong¹

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
2. College of Chemistry and Environmental Engineering, Chongqing University of Arts and Sciences, Yongchuan, Chongqing 402160, China

Received:2023-05-08 Revised:2023-08-17 Online:2023-11-15 Published:2023-12-01

摘要/Abstract

摘要： 土壤线虫是土壤循环重要的环节，对土壤生态系统具有重要影响。近年来，高通量测序技术因其分辨率和灵敏度高、测序范围广等优势，在线虫鉴定方面逐渐受到关注。目前关于高通量测序技术和形态学鉴定结果的一致性研究，仍比较欠缺。为了更好地比较两种分析方法，本研究同时采用形态学鉴定和高通量测序技术，对黄河上游区门源县不同斑块化退化高寒草甸土壤线虫群落组成和结构进行了测定和比较。结果表明两种分析方法在线虫群落丰度测定上具有显著差异，但在生物多样性的趋势的判断上差异不大。尽管高通量测序技术为快速洞察线虫多样性提供了有效的技术手段，但仍存在一些局限性导致其测定结果准确性、与形态学鉴定结果的一致性较差，通过高通量测序数据库的改进，可以进一步提高分子技术获取生态信息的准确性。

关键词: 形态学鉴定, 高通量测序, 土壤线虫, 群落结构, 斑块化高寒草甸

Abstract: Soil nematodes occupy the central link of the soil food web and have an important impact on soil ecosystems. High-throughput sequencing technology has gradually gained much attention in nematode identification due to its advantages such as high resolution,sensitivity and wide sequencing range. However,the concordance study between high-throughput sequencing technology and morphological identification results is still lack. In order to better understand the two analytical methods,this study was conducted to determine and compare the composition and structure of soil nematode communities in different patches of degraded alpine meadows in Menyuan County of the upper Yellow River region using morphological identification and high-throughput sequencing techniques. The results showed that the abundance of nematode communities had significant differences under different patches of degraded alpine meadows in Menyuan County of the upper Yellow River area and the differences were not significant in the determination of biodiversity. Although high-throughput sequencing technology provided an effective technical tool for rapid insight into nematode diversity,there were still some limitations that lead to poor accuracy of the measurement results and poor consistency with the results of morphological identifications. The improvement of high-throughput sequencing database could further improve the accuracy of ecological information obtained by molecular techniques.

Key words: Morphological identification, High-throughput sequencing, Soil nematodes, Community structure, Patchy alpine meadow

中图分类号:

Q938.1+3

陈国榕, 李希来, 李成一, 杨鹏年, 佘延娣, 吴勇. 黄河上游区斑块化退化高寒草甸土壤线虫群落分布特征[J]. 草地学报, 2023, 31(11): 3258-3270.

CHEN Guo-rong, LI Xi-lai, LI Cheng-yi, YANG Peng-nian, SHE Yan-di, WU Yong. Characteristics of Soil Nematode Community in the Different Degraded Patch of Alpine Meadow in the upper Yellow River Region[J]. Acta Agrestia Sinica, 2023, 31(11): 3258-3270.

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参考文献

[1] MIPAM T D,CHEN S,LIU J,et al. Short-term yak-grazing alters plant-soil stoichiometric relations in an alpine meadow on the eastern Tibetan Plateau[J]. Plant and Soil,2019(12):1-13
[2] 张宇鹏,吴笑天,李希来,等. 黄河源流域单元退化高寒草甸空间分布及其对土壤理化性质的响应[J]. 草地学报,2022,30(3):503-512
[3] SUN H F,LI X L,JIN L Q,et al. Effects of biological soil crusts on soil labile organic carbon of patchy alpine meadows in the Source Zone of the Yellow River,West China[J]. Catena,2023(220):106715
[4] ChEN J,YI S,QIN Y. The contribution of plateau pika disturbance and erosion on patchy alpine grassland soil on the Qinghai-Tibetan Plateau:Implications for grassland restoration[J]. Geoderma,2017(297):1-9
[5] 孙华方,李希来,金立群,等. 黄河源区斑块化退化高寒草甸土壤细菌群落多样性变化[J]. 环境科学,2022,43(9):4662-4673
[6] HOOGEN J,GEISEN S,ROUTH D,et al. Soil nematode abundance and functional group composition at a global scale[J]. Nature,2019,572(7768):194-198
[7] LIU T M,PENG S,WANG L L,et al. Contrasting effects of nitrogen deposition and increased precipitation on soil nematode communities in a temperate forest[J]. Soil Biology & Biochemistry,2020,148(1):107869
[8] 侯林秀,张斌,孙浩宇,等. 围封、放牧、刈割对内蒙古典型草原土壤线虫群落的影响[J]. 草地学报,2022,30(7):1738-1747
[9] 刘艳方. 青藏高原高寒草地植物群落与土壤线虫相互关系研究[D]. 西宁:青海师范大学,2020:60-69
[10] WANG B,WU L J,ChEN D M,et al. Grazing simplifies soil micro-food webs and decouples their relationships with ecosystem functions in grasslands[J]. Global Change Biology,2020,26(2):960-970
[11] WANG K,XUE K,WANG Z S,et al. Accelerated temporal turnover of the soil nematode community under alpine grassland degradation[J]. Land Degrandation & Development,2023,34(4):1171-1181
[12] DU X F,LI Y B,HAN X,et al. Using high-throughput sequencing quantitatively to investigate soil nematode community composition in a steppe-forest ecotone[J]. Applied Soil Ecology,2020(152):103562
[13] OLIVEIRA C M G,JUDITH HUBSCHEN,BROWN D J F,et al. Phylogenetic Relationships Among Xiphinema and Xiphidorus Nematode Species from Brazil Inferred from 18S rDNA Sequences[J]. Journal of Nematology,2004,36(2):153-159
[14] GAO D D,WANG B J,ZHANG K L,et al. Effects of ecosystem disturbance on nematode communities in calcareous and red soils:Comparison of taxonomic methods[J]. Soil Biology & Biochemistry,2021,155(1):108162
[15] DU X F,LIU H W,LI Y B,et al. Soil community richness and composition jointly influence the multifunctionality of soil along the forest-steppe ecotone[J]. Ecological Indicators,2022(139):108900
[16] LI B,LI Y B,FANIN N,et al. Adaptation of soil micro-food web to elemental limitation:Evidence from the forest-steppe ecotone. Soil Biology and Biochemistry,2022(170):108698
[17] KERFAHI D,TRIPATHI B M, PORAZINSKA D L,et al. Do tropical rain forest soils have greater nematode diversity than High Arctic tundra? A metagenetic comparison of Malaysia and Svalbard[J]. Global Ecol Biogeography,2016,25(6):716-728
[18] DARBY B J,TODD T C,HERMAN M A. High-throughput amplicon sequencing of rRNA genes requires a copy number correction to accurately reflect the effects of management practices on soil nematode community structure[J]. Molecular Ecology,2013,22(21):5456-5471
[19] GAO Z W,LIU Z Y,WU Z H,et al. Soil nematode communities and functions:Ecological package restoration responses in damaged desert steppe open-pit mining ecosystems[J]. Land degradation & development,2022,33(7):1015-1028
[20] 孙翌昕,李英滨,李玉辉,等. 高通量测序技术在线虫多样性研究中的应用[J]. 生物多样性,2022,30(12):200-210
[21] SCHENK J,KLEINBOLTING N,TRAUNSPURGER W. Comparison of morphological,DNA barcoding,and metabarcoding characterizations of freshwater nematode communities[J]. Ecology and Evolution,2020(10):1-15
[22] TREONIS A M,UNANGST S K,KEPLER R M,et al. Characterization of soil nematode communities in three cropping systems through morphological and DNA metabarcoding approaches[J]. Scientific Reports,2018,8(1):2004
[23] GEISEN S,SNOEK L B,HOOVEN F T,et al. Integrating quantitative morphological and qualitative molecular methods to analyse soil nematode community responses to plant range expansion[J]. Methods in Ecology & Evolution,2018(9):1366-1378
[24] 宋梓涵,李希来,李杰霞,等. 高寒草甸不同扰动斑块植物功能群和根土复合体特征变化研究[J]. 生态科学,2022,41(1):31-38
[25] 尹文英. 中国土壤动物检索图鉴[M]. 北京:科学出版社,1998:51-89
[26] BONGERS T. The nematodes of the Netherlands[M]. Utrecht:Stiching Publisher of Royal Dutch Natuurhistorische As-sociation,1988:90-145
[27] BONGERS T. The maturity index:an ecological measure of environmental disturbance based on nematode species composition[J]. Oecologia,1990,83(1):14-19
[28] BOLGER A M,MARC L,BJOERN U. Trimmomatic:a flexible trimmer for Illumina sequence data[J]. Bioinformatics,2014 (15):2114-2120
[29] MAGO T,SALZBERG S L. FLASH:Fast Length Adjustment of Short Reads to Improve Genome Assemblies[J]. Bioinformatics,2011,27(21):2957-2963
[30] WANG Q. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Applied and Environmental Microbiology,2007(73):5261-5267
[31] NEHER D A. Role of Nematodes in Soil Health and Their Use as Indicators[J]. Journal of Nematology,2002,33(4):161-168
[32] SHANNON C E. The mathematical theory of communication[J]. Bell Labs Technical Journal,1950,3(9):31-32
[33] SIMPSON E. Measurement of species diversity[J]. Nature,1949(163):688
[34] LU Q,LIU T,WANG N,et al. A review of soil nematodes as biological indicators for the assessment of soil health[J]. Frontiers of Agricultural Science and Engineering,2020,7(3):49-55
[35] SIERIEBRIENNIKOV B,FERRIS H,DEGOEDE R G M. NINJA:an automated calculation system for nematode-based biological monitoring[J]. European Journal of Soil Biology,2014 (61):90-93
[36] VANDEPUTTE D,KATHAGEN G,D’HOE K,et al. Quantitative microbiome profiling links gut community variation to microbial load[J]. Nature,2017(551):507-511
[37] WANG B,ZHU Y H,CHEN X,et al. Even short-term revegetation complicates soil food webs and strengths their links with ecosystem functions[J]. Journal of Applied Ecology,2022,59(7):1721-1733
[38] 吴雨薇,罗珊,拉琼,等. 青藏高原三江源区不同恢复期高寒草甸土壤线虫群落演变[J]. 土壤,2022,54(3):532-538
[39] WANG H,LIU G,HUANG B,et al. Long-term nitrogen addition and precipitation reduction decrease soil nematode community diversity in a temperate forest[J]. Applied Soil Ecology,2021,162(5878):103895
[40] 王宇彤,牛克昌. 青藏高原高寒草甸土壤环境对线虫功能多样性的影响[J]. 生物多样性,2020,28(6):707-717
[41] WANG Z Q,GUO S L,SUN Q Q,et al. Soil organic carbon sequestration potential of artificial and natural vegetation in the hilly regions of Loess Plateau[J]. Ecological Engineering,2015 (82):547-554
[42] GUAN P,ZHANG X,YU J,et al. Variation of soil nematode community composition with increasing sand-fixation year of Caragana microphylla:Bioindication for desertification restoration[J]. Ecological Engineering,2015(81):93-101
[43] 毛小芳,李辉信,陈小云,等. 土壤线虫三种分离方法效率比较[J]. 生态学杂志,2004,23(3):149-151
[44] 王楠,黄菁华,霍娜,等. 宁南山区不同植被恢复方式下土壤线虫群落特征:形态学鉴定与高通量测序法比较[J]. 生物多样性,2021,29(11):1513-1529
[45] DARBY B J,TODD T C,HERMAN M A. High-throughput amplicon sequencing of rRNA genes requires a copy number correction to accurately reflect the effects of management practices on soil nematode community structure[J]. Molecular Ecology,2013,22(21):5456-5471
[46] FLOYD R,ABEBE E,PAPERT A,et al. Molecular barcodes for soil nematode identification[J]. Molecular Ecology,2010,11(4):839-850
[47] 王楠,黄菁华,霍娜,等. 黄土高原半干旱区不同林龄柠条人工林土壤线虫群落特征[J]. 生态学杂志,2022,41(2):236-245
[48] SANCHEZ-MORENO S,JIMENEZ L,ALONSO-PRADOS J L,et al. Nematodes as indicators of fumigant effects on soil food webs in strawberry crops in Southern Spain[J]. Ecological Indicators,2010,10(2):148-156
[49] FERRIS H. Form and function:Metabolic footprints of nematodes in the soil food web[J]. European Journal of Soil Biology,2010,46(2):97-104
[50] YANG J,WU X,CHEN Y,et al. Combined attributes of soil nematode communities as indicators of grassland degradation[J]. Ecological Indicators,2021(131):108215
[51] LIANG W J,ZHONG S,HUA J F,et al. Nematode Faunal Response to Grassland Degradation in Horqin Sandy Land[J]. Pedosphere,2007,17(5):611-618
[52] COFFEY V. Legacies of afforestation on soil nematode community composition,structure,and diversity in a northern Canadian prairie[J]. Plant and Soil,2019(435):1a2
[53] ZHANG Z W,LI Q,ZHANG H Y,et al. The impacts of nutrientaddition and livestock exclosure on the soil nematode community in a degraded grassland[J]. Land Degradation& Development,2019,30(13):1574-1583(责任编辑刘婷婷)第31卷第11期 Vol.31 No. 11草地学报 ACTAAGRESTIASINICA 2023年 11月

黄河上游区斑块化退化高寒草甸土壤线虫群落分布特征

Characteristics of Soil Nematode Community in the Different Degraded Patch of Alpine Meadow in the upper Yellow River Region

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