草地学报

草地学报 ›› 2024, Vol. 32 ›› Issue (10): 3103-3112.DOI: 10.11733/j.issn.1007-0435.2024.10.010

• 研究论文 • 上一篇    

德兴矿区原生植物根际土壤真菌群落结构和多样性分析

张馨月1, 隋永超2, 王琼3, 杨越晴3, 李金波1, 李昶仪1, 许立新1   

  1. 1. 北京林业大学, 北京 100083;
    2. 北京格瑞林科技有限公司, 北京 100124;
    3. 矿冶科技集团有限公司, 北京 100160
  • 收稿日期:2023-12-11 修回日期:2024-05-08 发布日期:2024-11-04
  • 通讯作者: 许立新,E-mail:liberisa@163.com
  • 作者简介:张馨月(1999-)，女，汉族，山东烟台人，硕士研究生，主要从事裸露边坡植被恢复研究，E-mail:zhangxinyue430@126.com;隋永超(1994-)，男，汉族，北京人，硕士，主要从事裸露边坡植被恢复，E-mail:1815118314@qq.com
  • 基金资助:
    国家重点研发计划草种质资源重要性状基因型精准鉴定(2023YFD12000302);国家重点研发计划典型酸性废石堆场及周边污染土壤的修复技术集成及示范(2019YFC1805005)资助

Analysis of Fungal Community Structure and Diversity in Rhizosphere Soil of Native Plants in Dexing Mining Area

ZHANG Xin-yue1, SUI Yong-chao2, WANG Qiong3, YANG Yue-qing3, LI Jin-bo1, LI Chang-yi1, XU Li-xin1   

  1. 1. Beijing Forestry University, Beijing 100083, China;
    2. Beijing Greelin Technology Company, Beijing 100124, China;
    3. Beijing General Research Institute of Mining and Metallurgy, Beijing 100160, China
  • Received:2023-12-11 Revised:2024-05-08 Published:2024-11-04

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摘要： 为了解德兴废石堆场原生植物根际土壤真菌群落组成、分析群落结构与土壤理化性质之间的关系，本研究采用高通量测序技术分析矿区的金星蕨(Parathelypteris glanduligera)、紫金牛(Ardisia japonica)、太平莓(Rubus pacificus)和芒草(Miscanthus sinensis)根际真菌群落多样性。结果表明，4种原生植物根际土壤理化性质差异显著。4种植物根际土壤的优势真菌属于担子菌门和子囊菌门。主成分分析显示，4种原生植物根际土壤真菌群落结构存在差异，造成差异的指示属主要有金星蕨的红菇属，紫金牛的古根菌属和毛舌菌属，芒草的棉革菌属和色孢菌属。芒草的根际真菌群落丰富度和多样性最高。相关性分析表明，铅Pb和速效磷是影响根际真菌群落变化的主要环境因子。共生营养型真菌是金星蕨和太平莓根际土壤中的优势功能菌群。研究结果有助于了解德兴铜矿原生植物根际真菌生态功能，为废石堆场生态重建和植被恢复及筛选修复菌群提供了理论依据。

关键词: 土壤真菌, 高通量测序, 根际土, 矿区植物

Abstract: To understand the fungal community structure in the rhizosphere soil of native plants in Dexing waste rock stockpiling field,the relationship between fungal community structure and soil physicochemical properties was analyzed. Illumina Miseq PE250 high-throughput sequencing technology was used to analyze the fungal diversity of rhizosphere soil of four plants, Parathelypteris glanduligera,Ardisia japonica,Rubus pacificus,Miscanthus sinensis. Results showed that there were significant differences in the physicochemical properties of the rhizosphere soil of the four native plants. The dominant phyla in the rhizosphere soils of four plants were Basidiomycota and Ascomycota. Principal component analysis showed that there were significant differences among the rhizosphere soil fungal community structure of the four native plants in the mining area. The indicator genera associated with the differences are mainly Russula for Parathely pteris glanduligera,Archaeorhizomyces and Trichoglossum for Ardisia japonica,Tomentella and Callistosporium for Miscanthus sinensis. The rhizosphere fungal community of Miscanthus sinensis had the highest richness and diversity. RDA analysis showed that Pb and available phosphorus were the main driving factors affecting the dominant rhizosphere fungal community structure. The FUNGuild function prediction showed that functional fungal for rhizosphere soil of Parathely pteris glanduligera and Rubus pacificus was mainly Symbiotroph. Above results provided a better understanding of soil fungal functions of native plants in Dexing waste rock stockpiling field,as well as the theoretical bases for artificial cultivation microorganisms and reconstruction in waste rock stockpiling field.

Key words: Soil fungi, High-throughput sequencing, Rhizosphere soils, Mining plants

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