草地学报 ›› 2025, Vol. 33 ›› Issue (10): 3280-3290.DOI: 10.11733/j.issn.1007-0435.2025.10.016

• 研究论文 • 上一篇    

免耕补播对中度退化草地植被及土壤细菌群落特征的影响

刘海强1, 李世雄1,2,3, 赵文1, 刘晶晶4, 徐海峰1, 尹亚丽1   

  1. 1. 青海大学畜牧兽医科学院, 青海 西宁 810016;
    2. 三江源区高寒草地生态教育部重点实验室/青海大学, 青海 西宁 810016;
    3. 青海省高寒草地适应性管理重点实验室/青海大学, 青海 西宁 810016;
    4. 甘肃省平凉市林业科学研究所, 甘肃 平凉 730030
  • 收稿日期:2024-11-29 修回日期:2025-01-19 发布日期:2025-10-17
  • 通讯作者: 李世雄,E-mail:shixionglee@126.com;尹亚丽,E-mail:yaliyin@163.com
  • 作者简介:李世雄,E-mail:shixionglee@126.com;尹亚丽,E-mail:yaliyin@163.com
  • 基金资助:
    青海省科技计划项目(2023-ZJ-921M);国家自然科学基金(32260357)资助

Effects of No-tillage Reseeding on Community Characteristics of Vegetation and Microorganisms in Moderately Degraded Alpine Meadows

LIU Hai-qiang1, LI Shi-xiong1,2,3, ZHAO Wen1, LIU Jing-jing4, XU Hai-feng1, YIN Ya-li1   

  1. 1. Academy of Animal Science and veterinary, Qinghai University, Xining, Qinghai Province 810016, China;
    2. Key Laboratory of Alpine Grassland Ecosystem in the Three-River-Source, Ministry of Education, Qinghai University, Xining, Qinghai Province 810016, China;
    3. Qinghai Provincial Key Laboratory of Adaptive Management on Alpine Grassland, Qinghai University, Xining, Qinghai Province 810016, China;
    4. Institute of Forestry Sciences, Pingliang, Gansu Province 730030, China
  • Received:2024-11-29 Revised:2025-01-19 Published:2025-10-17

摘要: 为明确免耕补播对高寒退化草地植被及土壤细菌群落特征的影响,本研究在祁连山高寒草地生态试验站以中度退化高寒草地为对照,免耕补播青海草地早熟禾(Poa pratensis L.)为处理,对草地土壤细菌物种组成、群落结构及其调控因子等进行研究。结果表明:免耕补播后植被生物量和物种丰富度显著升高,禾本科重要值显著增加;土壤含水量、有机质碳、全氮、硝态氮和速效磷含量显著增加;而氨态氮的含量显著降低。土壤细菌群落物种组成以及群落多样性均未发生显著的改变。但是,免耕补播改变了原有的土壤细菌结构,在门水平,放线菌门和绿弯菌门的丰度显著降低;在科水平,WD2101 soil group和芽孢杆菌科的丰度显著升高。植被生物量、土壤pH值、有机碳和速效养分是引起土壤细菌群落结构变化的主要调控因子。综上,免耕补播具备在短期内修复草地植被,提高草地土壤肥力,改善土壤微环境的能力。

关键词: 免耕补播, 中度退化高寒草地, 植被群落特征, 土壤理化性质, 土壤细菌群落结构

Abstract: In order to determine the effects of no-tillage reseeding on the vegetation and soil bacterial community characteristics of alpine meadows, the species composition, community structure and regulatory factors of soil bacteria in the grassland were studied in the alpine meadow ecological experimental station of Qilian Mountains, with the moderately degraded alpine meadows as the control and the grass field of Poa pratensis ‘Qinghai’ with no-tillage reseeding. The results showed that the vegetation biomass and species richness and the important value of Gramineae increased significantly after no-tillage reseeding. Besides, soil water content, organic carbon, total nitrogen, nitrate nitrogen and available phosphorus contents increased significantly. The content of ammonium nitrogen decreased significantly. The species composition and diversity of soil bacterial community did not change significantly. However, no-tillage reseeding changed the original soil microflora structure, and the abundance of Actinobacteria and Chloroflexi decreased significantly at the phylum level. At the family level, the abundance of WD2101 soil group and Blastocatellaceae increased significantly. Vegetation biomass, soil pH, organic carbon and available nutrients were the main regulatory factors that induced changes in soil bacterial community structure. In summary, no-tillage reseeding had the ability to restore grassland vegetation, and improve soil fertility and soil microenvironment in a short period.

Key words: No-tillage reseeding, Moderately degraded alpine meadows, Vegetation community characteristics, Soil physicochemical properties, Soil bacterial community structure

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