草地学报

草地学报 ›› 2025, Vol. 33 ›› Issue (11): 3732-3740.DOI: 10.11733/j.issn.1007-0435.2025.11.024

• 研究论文 • 上一篇    下一篇

2，4-表油菜素内酯对盐胁迫下无芒雀麦生理特性的影响

周一凡, 杨钊, 隋晓青, 郎梦卿, 吉尔尔格, 刘海军, 叶忆真   

  1. 1. 新疆农业大学草业学院/西部干旱荒漠区草地资源与生态教育部重点实验室/新疆草地资源与生态重点实验室, 新疆 乌鲁木齐 830052
  • 收稿日期:2024-12-21 修回日期:2025-03-11 出版日期:2025-11-15 发布日期:2025-11-13
  • 通讯作者: 隋晓青,E-mail:sxq303@xjau.edu.cn
  • 作者简介:周一凡（2001-），女，汉族，新疆阿克苏人，硕士研究生，主要从事牧草栽培与种子生产研究，E-mail：1276176231@qq.com；
  • 基金资助:
    新疆维吾尔自治区重点研发专项（2023B02031）；国家牧草产业技术体系（CARS-34）资助

Effect of 2，4-Epibrassinolide on the Physiological Properties of Bromus inermis Under Salt Stress

ZHOU Yi-fan, YANG Zhao, SUI Xiao-qing, LANG Meng-qing, Jirge, LIU Hai-jun, YE Yi-zhen   

  1. 1. College of Grassland, Xinjiang Agricultural University, Key Laboratory of Grassland Resources and Ecology, Ministry of Education, Western Arid Desert Region, Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi 830052, Xinjiang, China
  • Received:2024-12-21 Revised:2025-03-11 Online:2025-11-15 Published:2025-11-13

PDF

摘要： 本研究以‘乌苏1号’无芒雀麦（Bromus inermis ‘Wusu No.1’）为试验材料通过预实验确定100 mmol·L-1 NaCl为盐胁迫浓度，采用盆栽试验，于盐胁迫条件下分别喷施不同浓度（0.01，0.1，1和10 μmol·L-1）的2，4-表油菜素内酯（2，4-Epibrassinolide， EBR），通过测定无芒雀麦幼苗的表型性状、抗氧化酶活性和渗透调节物质含量，探究外源EBR在盐胁迫下对无芒雀麦幼苗生理特性的影响。结果表明：100 mmol·L-1 NaCl盐胁迫显著抑制了无芒雀麦的株高、叶长、叶面积，而在盐胁迫下喷施EBR后可显著提高无芒雀麦幼苗的超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化物酶（Peroxidase，POD）、过氧化氢酶（Catalase，CAT）活性及渗透调节物质含量，显著降低丙二醛（Malondialdehyde，MDA）含量。通过综合评价分析确定在NaCl盐胁迫下喷施0.1 μmol·L-1 EBR可以有效促进无芒雀麦幼苗生长发育，减轻盐胁迫造成的膜脂过氧化伤害。本研究结果可为增强无芒雀麦抗盐能力提供理论依据和参考。

关键词: 无芒雀麦, 盐胁迫, 2, 4-表油菜素内酯, 抗氧化酶, 渗透调节

Abstract: This study used smooth bromegrass （Bromus inermis ‘Wusu No.1’） as the experimental material. The preliminary experiment selected 100 mmol·L-1 NaCl as the salt stress， and a pot experiment was conducted. Under salt stress conditions， with foliar application of different concentrations （0.01， 0.1， 1 and 10 μmol·L-1） of 2，4-epibrassinolide （EBR）. By measuring the phenotypic traits， antioxidant enzyme activities and osmotic adjustment substance contents of smooth bromegrass seedlings， the effects of exogenous EBR on the physiological characteristics of smooth bromegrass seedlings under salt stress were explored. The results showed that 100 mmol·L-1 NaCl salt stress significantly inhibited the plant height， leaf length and leaf area of smooth bromegrass seedlings. However， spraying EBR under salt stress could significantly increase the activities of superoxide dismutase， peroxidase and catalase， as well as the contents of osmotic adjustment substances， and significantly reduce the content of malondialdehyde. Through comprehensive evaluation and analysis， it was determined that spraying 0.1 μmol·L-1 EBR under NaCl salt stress could effectively promote the growth and development of smooth bromegrass seedlings and alleviate the membrane lipid peroxidation damage caused by salinity stress， thus providing a theoretical basis and reference for the enhancement of smooth bromegrass’s ability to resist salt.

Key words: Bromus inermis, Salt stress, 2, 4-epibrassinolide, Antioxidant enzymes, Osmotic adjustment

中图分类号: