星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析

doi:10.11733/j.issn.1007-0435.2026.07.003

草地学报 ›› 2026, Vol. 34 ›› Issue (7): 2366-2376.DOI: 10.11733/j.issn.1007-0435.2026.07.003

• 研究论文 • 上一篇

星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析

马蓉¹, 杨攀¹, 张蕴文², 胡晓炜¹, 王会², 王沛¹

1. 西南民族大学四川若尔盖高寒湿地生态系统国家野外科学观测研究站, 草地资源学院, 四川成都 610041;
2. 西南民族大学畜牧兽医学院, 四川成都 610041

收稿日期:2025-11-03 修回日期:2025-12-14 发布日期:2026-07-02
通讯作者: 王沛，E-mail:wangpei@swun.edu.cn
作者简介:马蓉（2000-），女，回族，宁夏中宁人，硕士研究生，主要从事牧草逆境生理与分子生物学研究，E-mail:18328569843@163.com
基金资助:
四川省科技计划项目（2024NSFSC0310）；西南民族大学“双一流”项目（CX2023013）；西南民族大学青藏高原研究院创新团队项目（2024CXTD11）资助

Cloning of PtCASP1 Gene in Puccinellia tenuiflora and Analysis of its Expression Patterns under Saline and Stagnant Stress

MA Rong¹, YANG Pan¹, ZHANG Yun-wen², HU Xiao-wei¹, WANG Hui², WANG Pei¹

1. National Field Observation and Research Station(Sichuan Zoige) for Alpine Wetland Ecosystem, College of Grass land resources, Southwest Minzu University, Chengdu, Sichuan Province 610041, China;
2. College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu, Sichuan Province 610041, China

Received:2025-11-03 Revised:2025-12-14 Published:2026-07-02

摘要/Abstract

摘要： 星星草（Puccinellia tenuiflora）是一种对盐涝交互逆境有独特适应性的盐生植物，内皮层加厚形成的质外体屏障可能是其适应这一环境的关键。凯氏带和木栓层是根内皮层水和溶质径向运输的质外体屏障。本研究以星星草为材料，通过基因克隆获得凯氏带形成关键基因 PtCASP1，并对该基因进行生物信息学分析、亚细胞定位及盐、涝和盐涝复合胁迫下的表达模式分析。结果表明，PtCASP1基因全长 552 bp，编码184个氨基酸，为疏水性稳定蛋白，具有响应赤霉素、干旱、低温及光反应等顺式作用元件。系统进化分析表明PtCASP1与多花黑麦草（Lolium multiflorum）、看麦娘（Alopecurus aequalis）的亲缘关系较近。免疫荧光分析显示，PtCASP1 蛋白分布于细胞核与细胞膜。PtCASP1 基因在根中表达量显著高于叶，且受盐、涝及盐涝复合胁迫诱导，均在各处理 72 h 时表达量最高。本研究可为今后深入研究凯氏带在星星草适应盐涝交互逆境中的功能提供参考。

关键词: 星星草, PtCASP1, 基因克隆, 亚细胞定位, 表达分析, 盐涝复合胁迫

Abstract: Puccinellia tenuiflora is a halophyte with remarkable adaptability to the combined stress of saline and stagnant. The apoplastic barrier formed by endodermal thickening is likely crucial for this adaptation， in which the Casparian strip and suberin lamellae serve as key apoplastic barriers in endodermis for the radial transport of water and solutes. In this study， using Puccinellia tenuiflora as material， we cloned PtCASP1， a key gene involved in Casparian strip formation. Bioinformatic analysis， subcellular localization， and expression pattern analysis under saline， stagnant and combined saline-stagnant stresses were conducted. The results showed that the PtCASP1 gene was 552 bp in length， encoding 184 amino acids. It was a hydrophobic， stable protein and contained cis-acting elements responsive to gibberellin， drought stress， low temperature stress， and light response. Phylogenetic analysis indicated that PtCASP1 was closely related to those in Lolium multiflorum and Alopecurus aequalis. Immunofluorescence analysis revealed that the PtCASP1 protein exhibited dual localization in the nucleus and cell membrane. The expression level of the PtCASP1 gene in the roots of Puccinellia tenuiflora was significantly higher than that in the leaves， and it responded to saline， stagnant， and combined saline-stagnant stresses， with the highest relative expression levels at 72 hours post-treatment for all stress conditions. This study provides a reference for further investigation into the function of the Casparian strip in helping Puccinellia tenuiflora adapt to saline-stagnant combined stress.

Key words: Puccinellia tenuiflora, PtCASP1, Gene cloning, Subcellular localization, Expression analysis, Combined saline-stagnant stress

中图分类号:

Q943

马蓉, 杨攀, 张蕴文, 胡晓炜, 王会, 王沛. 星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析[J]. 草地学报, 2026, 34(7): 2366-2376.

MA Rong, YANG Pan, ZHANG Yun-wen, HU Xiao-wei, WANG Hui, WANG Pei. Cloning of PtCASP1 Gene in Puccinellia tenuiflora and Analysis of its Expression Patterns under Saline and Stagnant Stress[J]. Acta Agrestia Sinica, 2026, 34(7): 2366-2376.

参考文献

[1] ROZEMA J, FLOWERS T. Crops for a salinized world[J]. Science, 2008, 322(5907):1478-1480
[2] VAN ZELM E, ZHANG Y X, TESTERINK C. Salt tolerance mechanisms of plants[J]. Annual Review of Plant Biology, 2020, 71:403-433
[3] ZHANG H M, ZHU J H, GONG Z Z, et al. Abiotic stress responses in plants[J]. Nature Reviews Genetics, 2022, 23(2):104-119
[4] MUNNS R, TESTER M. Mechanisms of salinity tolerance[J]. Annual Review of Plant Biology, 2008, 59:651-681
[5] BARRETT-LENNARD E G. The interaction between waterlogging and salinity in higher plants: causes, consequences and implications[J]. Plant and Soil, 2003, 253(1):35-54
[6] ZHANG X, WEI L Q, WANG Z Z, et al. Physiological and molecular features of Puccinellia tenuiflora tolerating salt and alkaline-salt stress[J]. Journal of Integrative Plant Biology, 2013, 55(3):262-276
[7] ZHANG W T, LIU J, ZHANG Y X, et al. A high-quality genome sequence of alkaligrass provides insights into halophyte stress tolerance[J]. Science China Life Sciences, 2020, 63(9):1269-1282
[8] HAO Y B. Study on the morphological structure and physiological mechanism of Puccinellia tenuiflora due to the combined effect of salt and water stress [D]. Chengdu:Southwest Minzu University, 2023:35-37 郝彦博. 小花碱茅适应盐涝交互逆境的结构基础和生理机制研究[D]. 成都:西南民族大学, 2023:35-37
[9] GELDNER N. The endodermis[J]. Annual Review of Plant Biology, 2013, 64:531-558
[10] ZHUANG Y, LI L. Are cuproproteins part of the multi-protein framework for making the Casparian strip？[J]. Plant Signaling & Behavior, 2020, 15(10):1798605
[11] NASEER S, LEE Y, LAPIERRE C, et al. Casparian strip diffusion barrier in Arabidopsis is made of a lignin polymer without suberin[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(25):10101-10106
[12] BARBERON M, VERMEER J E M, DE BELLIS D, et al. Adaptation of root function by nutrient-induced plasticity of endodermal differentiation[J]. Cell, 2016, 164(3):447-459
[13] BARBERON M. The endodermis as a checkpoint for nutrients[J]. New Phytologist, 2017, 213(4):1604-1610
[14] CUI Y N, MAN Y, SONG C W, et al. Chemical components, physiological functions and regulation mechanism of plant Casparian strips[J]. Science in China (Series), 2020, 50(2):102-110 崔亚宁, 满奕, 宋程威, 等. 植物凯氏带化学成分、生理功能及相关调控机制的研究进展[J]. 中国科学(生命科学), 2020, 50(2):102-110
[15] KOTULA L, RANATHUNGE K, SCHREIBER L, et al. Functional and chemical comparison of apoplastic barriers to radial oxygen loss in roots of rice (Oryza sativa L.) grown in aerated or deoxygenated solution[J]. Journal of Experimental Botany, 2009, 60(7):2155-2167
[16] KRISHNAMURTHY P, RANATHUNGE K, FRANKE R, et al. The role of root apoplastic transport barriers in salt tolerance of rice (Oryza sativa L.)[J]. Planta, 2009, 230(1):119-134
[17] KARAHARA I, IKEDA A, KONDO T, et al. Development of the Casparian strip in primary roots of maize under salt stress[J]. Planta, 2004, 219(1):41-47
[18] ROPPOLO D, DE RYBEL B, TENDON V D, et al. A novel protein family mediates Casparian strip formation in the endodermis[J]. Nature, 2011, 473(7347):380-383
[19] WANG Z G, YAMAJI N, HUANG S, et al. OsCASP1 is required for casparian strip formation at endodermal cells of rice roots for selective uptake of mineral elements[J]. The Plant Cell, 2019, 31(11):2636-2648
[20] YANG J H, DING C Q, XU B C, et al. A Casparian strip domain-like gene, CASPL negatively alters growth and cold tolerance[J]. Scientific Reports, 2015, 5:14299
[21] XUE B P, LIANG Z C, LI D Y, et al. Genome-wide identification and expression analysis of CASPL gene family in Zea mays (L.)[J]. Frontiers in Plant Science, 2024, 15:1477383
[22] KOTULA L, CLODE P L, STRIKER G G, et al. Oxygen deficiency and salinity affect cell-specific ion concentrations in adventitious roots of barley (Hordeum vulgare)[J]. New Phytologist, 2015, 208(4):1114-1125
[23] WIENGWEERA A, GREENWAY H, THOMSON C J. The use of agar nutrient solution to simulate lack of convection in waterlogged soils[J]. Annals of Botany, 1997, 80(2):115-123
[24] XUE B P, LIANG Z C, LIU Y, et al. Comparative analysis of casparian strip membrane domain protein family in Oryza sativa (L.) and Arabidopsis thaliana (L.)[J]. International Journal of Molecular Sciences, 2024, 25(18):9858
[25] GUO Q, WANG P, MA Q, et al. Selective transport capacity for K⁺ over Na⁺ is linked to the expression levels of PtSOS1 in halophyte Puccinellia tenuiflora[J]. Functional Plant Biology, 2012, 39(12):1047-1057
[26] ZHANG J H. Biologigal function analysis of ZmCASPL2B2 in response to salt tolerance in maize[D]. Wuhan:Huazhong Agricultural University, 2022:9-10 张锦辉. ZmCASPL2B2响应玉米盐胁迫的生物学功能分析[D]. 武汉:华中农业大学, 2022:9-10
[27] WANG Z G. The mechanism of OsCASP1 in casparian strip formation at the endodermis and controlling root uptake of mineralelements in rice[D]. Nanning:Guangxi University, 2022:10-12 王志刚. OsCASP1在水稻根内皮层凯氏带形成和矿质元素吸收中的作用机理[D]. 南宁:广西大学, 2022:10-12
[28] LI D N, WANG K, TAO J L, et al. Cloning, subcellular localization and expression analysis of MsMYB44 gene of Medicago sativa ‘Pianguan’[J]. Acta Agrestia Sinica, 2026, 34(1):33-42 李迪娜, 王凯, 陶佳丽, 等. 偏关苜蓿MsMYB44基因的克隆、亚细胞定位及表达分析[J]. 草地学报, 2026, 34(1):33-42
[29] FEI Z Z. Analysis and functional identification of CASP family members in wheat[D]. Wuhan:Huazhong Agricultural University, 2021:48-49 费甄臻. 小麦CASP家族成员分析及功能鉴定[D]. 武汉:华中农业大学, 2021:48-19

星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析

Cloning of PtCASP1 Gene in Puccinellia tenuiflora and Analysis of its Expression Patterns under Saline and Stagnant Stress

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