草地学报

›› 2005, Vol. 13 ›› Issue (1): 30-33.DOI: 10.11733/j.issn.1007-0435.2005.01.008

• 研究论文 • 上一篇    下一篇

蒙农杂种冰草成熟胚愈伤组织的诱导及植株再生

张辉1, 魏建华2, 霍秀文1, 徐春波1, 米福贵1, 云锦凤1   

  1. 1. 内蒙古农业大学, 呼和浩特, 010018;
    2. 北京农业生物技术研究中心, 北京, 100089
  • 收稿日期:2003-12-18 修回日期:2004-04-15 出版日期:2005-02-15 发布日期:2005-02-15
  • 通讯作者: 米福贵;云锦凤
  • 作者简介:张辉(1978- ),男,内蒙古包头市人,硕士研究生,研究方向为牧草种质资源与育种
  • 基金资助:
    国家转基因植物研究与产业化专项资助(J-2002-B-008)

A Study on the Induction of Embryogenic Callus and Plant Regeneration from Mature Embryos of Agropyron cristatum? Agropyron desertorum cv. Mengnong

ZHANG Hui11, WEI Jian-hua2, HUO Xiu-wen1, XU Chun-bo1, MI Fu-gui1, YUN Jin-feng1   

  1. 1. Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010018, China;
    2. Beijing Center of Agro-Biotechnology, Beijing 100089, China
  • Received:2003-12-18 Revised:2004-04-15 Online:2005-02-15 Published:2005-02-15

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摘要： 以蒙农杂种冰草成熟胚为外植体,通过胚性愈伤组织的诱导进行植株再生,结果表明:在附加甘露醇(0.2mol/L)和2,4-D(2.0mg/L)的MS培养基中,冰草成熟胚可诱导产生愈伤组织,诱导率为87.6%,但质量较差;将其在降低2,4-D浓度和添加6-BA的继代培养基中继代改造,可明显改善愈伤组织质量,增加胚性愈伤;将筛选改良的愈伤组织置于分化培养基MS+ZT(3.0mg/L)+NAA(1.0mg/L)中,分化率为82%;分化小苗在1/2MS培养基上可生根并得到完整植株.本研究还建立了一套有效的以成熟胚为外植体的蒙农杂种冰草组织培养再生体系.

关键词: 蒙农杂种冰草, 成熟胚, 愈伤组织, 植株再生

Abstract: A study using the mature embryos of wheatgrass (Agropyron cristatum?Agropyron desertorum cv.Mengnong) as transplants and embryogenic callus induction to regenerate plants shows that the callus could be induced from mature embryos with a frequency of 87.6% on the MS +mannite (0.2 mol/L)+2,4-D(2.0 mg/L) medium, though the quality of the callus is poor. Subcultured on the medium with reduced 2,4-D and 6-BA added, the quality of the callus could be improved and the embryogenic callus increased. Taking MS+ZT(3.0 mg/L)+NAA (1.0 mg/L) as the medium for differentiation, the distinguishing rate of improved callus reaches 82%. On the 1/2 MS medium free of hormone, plantlets develop roots and become complete plants. An effective system of wheatgrass (A.cristatum譇.desertorum cv.Mengnong) regeneration from mature embryos has been developed.

Key words: Wheatgrass (Agropyron cristatum?A.desertorum cv.Mengnong), Mature embryo, Embryogenic callus, Plant regeneration

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