用RAPD标记分析草地早熟禾遗传多样性

doi:10.11733/j.issn.1007-0435.2006.02.005

›› 2006, Vol. 14 ›› Issue (2): 120-123,128.DOI: 10.11733/j.issn.1007-0435.2006.02.005

用RAPD标记分析草地早熟禾遗传多样性

田志宏, 邱永福, 严寒, 何勇

长江大学生命科学学院, 湖北, 荆州, 434025

收稿日期:2005-08-25 修回日期:2006-02-17 出版日期:2006-05-15 发布日期:2006-05-15
作者简介:田志宏(1966- ),男,湖北监利人,教授,博士,主要从事草坪植物生物技术研究,E-mail:zhtian@yangtzeu.edu.cn
基金资助:
湖北省重点科技发展计划项目(992P0603);湖北省教育厅重大科研项目(99Z007);湖北省新世纪高层次人才工程科研项目(鄂人[2003]31号)资助

Genetic Diversity in Kentucky Bluegrass Based on Random Amplified Polymorphic DNA(RAPD) Analysis

TIAN Zhi-hong, QIU Yong-fu, YAN Han, HE Yong

College of Life Science, Yangtze University, Jingzhou, Hubei Province 434025, China

Received:2005-08-25 Revised:2006-02-17 Online:2006-05-15 Published:2006-05-15

摘要/Abstract

摘要： 采用RAPD分子标记技术对从美国引进的12份草地早熟禾(Poapra tensis L.)品种的遗传多样性进行分析。结果表明,从60个随机引物中筛选的17个有效引物共扩增出104条带,其中多态性带65条,占总带数的62.5%,平均每个引物扩增出多态性带3.82条;利用NTSY S-PC软件计算品种间Jaccard遗传相似系数(GS),变化范围为0.362~0.971;用非加权组法(UPGMA)聚类分析,建立供试品种的分子系统树状图,以相似系数0.7为标准分为5类,其中新哥来德(NuGlade)、解放者(Liberator)、浪潮(Impact)、午夜(Midnight)、自由Ⅱ(Freedom Ⅱ)、超级伊克利(Total eclipse)和纳苏(Nassau)共7个品种聚为一类,兰神(Nublue)和美洲王(America)聚为一类,其余品种各自为一类;该结果对引进草地早熟禾品种资源评价与利用具有一定的参考价值。

关键词: 草地早熟禾, 遗传多样性, RAPD, 聚类分析

Abstract: Kentucky bluegrass(Poa pratensis L.) is an important cool-season turfgrass adapted to a wide range of soils and climates in China.The genetic variation among varieties provides the theoretic and practical basis for variety extension and genetic improvement.An analysis of genetic diversity of the 12 cultivars of the kentucky bluegrass introduced from America was carried out,using the RAPD markers technology.Results show that 17 effective primers were screened from 60 arbitrary primers,and a total of 104 DNA bands were amplified;among which 65 DNA bands(62.5%) were polymorphic,the average polymorphic DNA bands amplified by each primer was 3.82.The range of Jaccard's genetic similarity coefficient among the cultivars computed by NTSYS-PC software changed within 0.362 to 0.971.A DNA molecular dendrogram was established for the 12 cultivars of kentucky bluegrass based on UPGMA analysis.The cultivars in the dendrogram were divided into five categories under the standard of 0.7 similarity coefficient.Among them,"NuGlade","Liberator","Impact","Midnight","Freedom Ⅱ","Total eclipse",and "Nassau" clustered together into one group;"Nublue" and "America" formed another;while the rest each formed its own category.The results are valuable for the estimation and development of the introduced cultivar resources of kentucky bluegrass.

Key words: Kentucky bluegrass, Genetic diversity, Random amplified polymorphic DNA, Cluster analysis

中图分类号:

S812
Q943

田志宏, 邱永福, 严寒, 何勇. 用RAPD标记分析草地早熟禾遗传多样性[J]. , 2006, 14(2): 120-123,128.

TIAN Zhi-hong, QIU Yong-fu, YAN Han, HE Yong . Genetic Diversity in Kentucky Bluegrass Based on Random Amplified Polymorphic DNA(RAPD) Analysis[J]. , 2006, 14(2): 120-123,128.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2006.02.005

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2006/V14/I2/120

参考文献

[1] 赵桂琴.早熟禾育种的研究进展与现状[J]. 甘肃农业大学学报,2000,35(2):119-126.
[2] 古长标,郭成宝,唐泉,等.17个草坪草品种在南京地区的引种试验[J]. 草业学报,1999,16(6):46-49.
[3] 李秋杰,田志宏,魏瑜.荆州市冷季型草坪草的引种栽培试验研究[J]. 湖北农学院学报,2001,21(4):318-322.
[4] 马智宏,李征,王北洪,等.冷季型草坪草耐旱及耐寒性比较[J]. 草地学报,2002,10(4):318-321.
[5] Williams J G K,Kubelik A R,Livak K J,et al.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J]. NuclAcidRes,1990,18(22):6531-6535.
[6] Mengistu L W,Mueller-Warrant G W,Barker R E.Genetic diversity of Poa annua in western Oregon grass seed crops[J]. Theor Appl Genet,2000,101(1-2):70-79.
[7] Casler M D,Rangel Y,Stier J C,et al.RAPD marker diversity among creeping bentgrass clone[J]. Crop Sci,2003,43 (2):688-693.
[8] 韩冰,王学敏,易津,等.驼绒藜属植物种质资源遗传多样性RAPD分析[J]. 草地学报,2003,11(2):154-158.
[9] 张红梅,赵萌莉,李青丰,等.内蒙古地区大针茅群体遗传多样性RAPD研究[J]. 草地学报,2003,11(2):170-177,188.
[10] 李亚,佟海英.中华结缕草遗传分化的RAPD分析[J]. 广西植物,2004,24(4):345-349,366.
[11] 李景欣,云锦凤,郭军.16个天然冰草种群遗传多样性RAPD分析[J]. 草地学报,2005,13(3):190-193.
[12] Ferdinandez Y S N,Somers D J,Coulman B E.Estimating the genetic relationship of hybrid bromegrass to smooth bromegrass and meadow bromegrass using RAPD markers[J]. Plant Breed,2001,120(2):149-153.
[13] 李阳春.早熟禾属植物种间关系的RAPD分析[J]. 草业学报,2002,11(4):94-99.
[14] 丁春邦,周永红,杨瑞武,等.应用RAPD分子标记探讨拟鹅观草属的种间关系[J]. 草业学报,2005,14(1):38-43.
[15] 解新明,卢小良.利用RAPD标记分析狼尾草属牧草品种间的遗传关系[J]. 草业学报,2005,14(2):52-56.
[16] Golembiewski R C,Danneberger T K,Sweeney P M.Potential of RAPD markers for use in the identification of Creeping Bentgrass cultivars[J]. Crop Sci,1997,37:212-214.
[17] Scheefa E A,Caslerb M D,Jung G.Development of species specific SCAR markers in bentgrass[J]. Crop Sci,2003,43(1):345-349.
[18] Edwards K,Johnstone C,Thompson C.A simple and rapid method for the preparation of plant genomic DNA for PCR analysis[J]. NuclAcid Res,1991,19(6):1349.
[19] 何川生,何兴金,葛颂,等.烤烟品种资源的RAPD分析[J]. 植物学报,2001,43(6):610-614.
[20] Stammers M,Harris J,Evans G M,et al.Use of random PCR (RAPD) technology to analysis phylogenetic relationships in the Lolium/Festuca complex[J]. Heredity,1995,74(1):19-27.
[21] 汪小全,邹喻苹,张大明,等.RAPD应用于遗传多样性和系统学研究中的问题[J]. 植物学报,1996,38(12):954-962.
[22] 邱永福,田志宏.湖北江汉平原冷季型草坪草的抗性研究[J]. 草业科学,2005,22(9):77-80.
[23] 邱永福,田志宏.湖北江汉平原冷地型草坪草的坪用特性研究[J]. 草业科学,2006,23(2):98-102.
[24] 柴明良.草坪草转基因研究进展[J]. 科技通报,2002,18(1):67-72.
[25] Porceddu A,Albertini E,Barcaccia G,et al.Linkage mapping in apomictic and sexual kentucky bluegrass (Poa pratensis L.)genotypes using a two way pseudo testcross strategy based on AFLP and SAMPL markers[J]. Theor Appl Genet,2002,104(2-3):273-280

用RAPD标记分析草地早熟禾遗传多样性

Genetic Diversity in Kentucky Bluegrass Based on Random Amplified Polymorphic DNA(RAPD) Analysis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	马骢毓, 韩重阳, 马赛男, 李旭旭, 蔡家邦, 汪阳, 张新全, 聂刚. 基于SSR分子标记的13个白三叶(Trifolium repens L.)品种指纹图谱构建[J]. 草地学报, 2021, 29(9): 1892-1899.
[2]	左郎, 王树森, 马迎梅, 温苏雅勒图, 白刚, 郁鑫明. 火炬树浸提液对两种草坪草种子萌发的影响[J]. 草地学报, 2021, 29(9): 1927-1933.
[3]	罗铭欣, 刘凤民, 陈纪言, 崔均涛, 张伟丽. ⁶⁰Coγ辐射柱花草M₃代的农艺性状及遗传多样性分析[J]. 草地学报, 2021, 29(9): 1992-2000.
[4]	梁小玉, 季杨, 胡远彬, 易军, 白史且, 张靓, 张新全. 菊苣EST-SSR分子标记开发及通用性分析[J]. 草地学报, 2021, 29(9): 2081-2090.
[5]	金一锋, 陈阳, 齐欣, 高岩松, 金忠民, 赵清峰, 王琦. 草地早熟禾NRT1/PTR FAMILY 8.3基因的克隆及表达分析[J]. 草地学报, 2021, 29(7): 1397-1405.
[6]	朱琨, 郑桂华, 刘丽杰, 周璐, 李珊珊, 范震宇, 钟玥, 尼尼. 草地早熟禾叶片表皮解剖结构与抗白粉病性的研究[J]. 草地学报, 2021, 29(7): 1430-1435.
[7]	张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.
[8]	刘凌云, 范希峰, 滕珂, 岳跃森, 常智慧, 武菊英. 苔草属植物遗传多样性研究进展[J]. 草地学报, 2021, 29(6): 1148-1157.
[9]	朱琨, 刘丽杰, 周璐, 李珊珊, 范震宇, 吴纪一, 尼尼. 外源激素对染白粉菌草地早熟禾叶片膜透性及表面结构的影响[J]. 草地学报, 2021, 29(6): 1210-1216.
[10]	陈志祥, 罗小燕, 李拴林, 吴如月, 王文强, 丁西朋. 基于SSR标记的木豆种质资源遗传多样性与群体结构分析[J]. 草地学报, 2021, 29(5): 904-911.
[11]	朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报, 2021, 29(4): 635-643.
[12]	张荟荟, 梁维维, 张学洲, 阿斯娅·曼力克, 朱昊, 魏鹏, 康帅, 李学森. 新疆野生老芒麦种质资源形态及生长特性分析[J]. 草地学报, 2021, 29(4): 701-708.
[13]	梁国玲, 刘文辉, 马祥. 590份皮燕麦种质资源穗部性状遗传多样性分析[J]. 草地学报, 2021, 29(3): 495-503.
[14]	张琦, 魏臻武, 闫天芳, 耿小丽. 燕麦种质资源农艺性状遗传多样性的鉴定评价[J]. 草地学报, 2021, 29(2): 309-316.
[15]	安明珠, 韩博, 姜华, 段新慧, 文亦芾, 周凯. 鸭茅物种起源与系统发育分析研究进展[J]. 草地学报, 2021, 29(12): 2637-2644.