草地学报

›› 2006, Vol. 14 ›› Issue (4): 315-318,327.DOI: 10.11733/j.issn.1007-0435.2006.04.004

• 研究论文 • 上一篇    下一篇

高羊茅叶斑病病原鉴定及生物学特性

郑莉1, 黄俊斌1, 向培建2   

  1. 1. 华中农业大学植物科技学院, 湖北省作物病害监测与安全控制重点实验室, 武汉, 430070, 中国;
    2. 加拿大圭尔夫大学, 环境生物系, 圭尔夫, 安大略省, N1G2W1, 加拿大
  • 收稿日期:2006-01-24 修回日期:2006-06-06 出版日期:2006-11-15 发布日期:2006-11-15
  • 通讯作者: 黄俊斌,E-mail:junb inhuang@mail.h zau.edu.cn
  • 作者简介:郑莉(1982- ),女,硕士研究生,研究方向为分子植物病理,E-mail:sue8244@webmail.hzau.edu.cn
  • 基金资助:
    国家外专局2003年度引智项目资助

Etiological and Biological Characterization of the Pathogenic Fungus Causing Leaf Spot on Festuca arundinacea

ZHENG Li1, HUANG Jun-bin1, Hsiang Tom2   

  1. 1. Dept.of Plant Protection, College of Plant Science and Technology, Key Lab of Plant Pathology of Hubei Province, Huazhong Agriculture University, Wuhan, Hubei Province 430070, China;
    2. Dept.of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
  • Received:2006-01-24 Revised:2006-06-06 Online:2006-11-15 Published:2006-11-15

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摘要： 从宜昌市夷陵区草坪型高羊茅(Festuca arundinacea.)分离出引起叶斑病的病原真菌,对该菌进行了形态学观察、致病性鉴定、核糖体DNA-ITS序列分析和生物学特性研究。结果表明:该病原菌能侵染高羊茅,在26℃和光照下,在PDA培养基上培养6d后菌落呈墨绿色,菌丝有隔,分生孢子有4个横隔膜,平均大小为30.6~17.0μm×10.9~13.6μm,中部3胞暗色,第3个细胞基部膨大;其核糖体DNA-ITS序列分析表明,该菌与GenBank中近缘弯孢(Curvularia affinis)的同源性是100%;结合形态学特征和致病性测定,认为该菌为C.affinis,这是C.affinis引起草坪型高羊茅叶斑病在我国的首次报道;生物学特性的研究表明,该菌菌丝生长的最适温度为25~30℃,最适pH范围为6~7,最适碳源为蔗糖,最适氮源为Ca(NO3)2;菌丝和分生孢子的致死温度分别为55℃10min和60℃10min。

关键词: 高羊茅, 近缘弯孢霉, 叶斑病, 病原鉴定, 生物学特性

Abstract: A pathogen was isolated from Festuca arundinacea of a turf in Yiling district of Yichang, Hubei Province. The morphological characteristics, pathogenicity, the DNA sequence of ribosomal ITS and biological characteristics of the pathogen were studied. In a test of pathogenicity, the organism was able to infect F.arundinacea in vitro. After cultivation on PDA at 26℃ under continuous fluorescent light for 6 days, the colonies were black green and the hyphae were septate. Spore size was 30.6~17.0 μm×10.9~13.6 μm, mostly with four-septa. The central cell was dark and the third cell from the base was the largest. The DNA sequence of ribosomal ITS of the isolates was found to match 100% with an ITS sequence of Curvularia affinis in GenBank. This is the first report of leaf spot caused by C.affinis on F.arundinacea in China. The studies on biological characteristic revealed that the optimal temperature for the mycelial growth was 25～30℃, and the optimal pH for mycelial growth was pH 5～7. Among carbon and nitrogen sources, sucrose and calcium nitrate were best for mycelial growth. The lethal temperature for mycelial growth and spores gemination were respectively 55℃ and 60℃ for 10 min.

Key words: Festuca arundinacea, Curvularia affinis, Leaf spot, Pathogen identification, Biological characteristics

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