草地学报

›› 2007, Vol. 15 ›› Issue (6): 577-581.DOI: 10.11733/j.issn.1007-0435.2007.06.013

• 研究论文 • 上一篇    下一篇

结缕草属植物种间关系的等位酶研究

周志芳1,2, 宣继萍2, 刘建秀2   

  1. 1. 南京农业大学园艺学院, 南京, 210095;
    2. 江苏省中科院植物研究所, 南京, 210014
  • 收稿日期:2006-12-14 修回日期:2007-07-28 出版日期:2007-12-15 发布日期:2007-12-15
  • 通讯作者: 刘建秀,E-mail:turfunit@yahoo.com.cn
  • 作者简介:周志芳(1982- ),女,硕士研究生,研究方向为草坪草资源多样性,E-mail:zhouzhifang1982@126.com
  • 基金资助:
    国家自然基金项目(30371011)资助

Isozyme Study on Interspecies Genetic Relationship of Zoysia Willd.

ZHOU Zhi-fang1,2, XUAN Ji-ping2, LIU Jian-xiu2   

  1. 1. College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China;
    2. Zhongshan Arboretum, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu Province 210014, China
  • Received:2006-12-14 Revised:2007-07-28 Online:2007-12-15 Published:2007-12-15

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摘要： 分析结缕草属(Zoysia Willd.)9种1变种共59份种源的苹果酸脱氢酶(MDH),酯酶(EST)及过氧化物酶(POD)三种等位酶特征,研究其种间亲缘关系,结果表明:①种内遗传分化大小依次为:沟叶结缕草(Z.matrella(L.)Merr.)>结缕草(Z.japonica Steud.)>中华结缕草(Z.sinica Hance)>朝鲜结缕草(Z.koreana Mez);②聚类结果显示:结缕草、中华结缕草、长花结缕草(Z.sinicavar.nipponica Ohwi)、大穗结缕草(Z.macrostachya Franch.)聚为一类;沟叶结缕草、细叶结缕草(Z.tenuifolia Willd.ex Trin.)先聚为一类,然后小结缕草(Z.minima Zotov)、太平洋结缕草(Z.pacifica Hotta ex Kuroki)、大花结缕草(Z.macrantha Desvaux)也聚到这一类;而朝鲜结缕草自成一类,聚类结果与外部性状特征和地理分布位置相统一;③结缕草属植物为开放式的外繁育系统,种内各种源间遗传变异大于种间变异。

关键词: 结缕草属, 种间关系, 等位酶

Abstract: The characteristics of three isozyme systems(MDH,EST,POD) were investigated for 59 accessions of Zoysia Willd.belonging to 9 species and 1 variety to study the genetic relationship of them.The results show as follows: the genetic diversity was ranked as: Z.matrella(L.) Merr.>Z.japonica Steud.>Z.sinica Hance>Z.koreana Mez;Z.japonica,Z.sinica, Z.sinica var.nipponia Ohwi,and Z.macrostachya Franch.were clustered together;Z.matrella,Z.tenuifolia Willd.ex Trin.were clustered to a group,then Z.minima Zotov,Z.pacifica Hotta ex Kuroki,and Z.macrantha Desvaux were also clustered to this group;Z.koreana was clustered to be a group by itself,the clustering result was consistent with the morphological characters and the geography distribution;Zoysia Willd.possesses the open breeding system,and the intraspecies genetic diversity was higher than interspecies.

Key words: Zoysia Willd., Genetic relationship, Isozyme

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