草地学报

›› 2009, Vol. 17 ›› Issue (2): 141-144.DOI: 10.11733/j.issn.1007-0435.2009.02.002

• 研究报告 • 上一篇    下一篇

白羊草真叶愈伤组织诱导和植株再生

董宽虎, 于娜, 程钰宏   

  1. 山西农业大学动物科技学院, 山西太谷, 030801
  • 收稿日期:2008-09-22 修回日期:2008-11-20 出版日期:2009-04-15 发布日期:2009-04-15
  • 作者简介:董宽虎(1956- ), 男, 山西偏关人, 博士, 教授, 博士生导师, 研究方向为草地资源与草地管理, E-mail:dongkuanhu@126.com
  • 基金资助:
    国家科技支撑计划项目(2007BAD56B01);农业部公益性行业(农业)科研专项(nyhyzx07-022)资助

Callus Induction and Plant Regeneration from Euphyllas of Bothriochloa ischaemum

DONG Kuan-hu, YU Na, CHENG Yu-hong   

  1. College of Animal Science and technology, Shanxi Agricultural University, Taigu, Shanxi Province, 030801, China
  • Received:2008-09-22 Revised:2008-11-20 Online:2009-04-15 Published:2009-04-15

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摘要： 以7日龄白羊草(Bothriochloa ischaemum(L.)Keng)真叶作为外植体,进行愈伤组织诱导和植株再生研究,筛选较合适的激素组合,以建立白羊草再生体系。结果表明:在愈伤诱导阶段KT的作用明显,其与2,4-D的交互作用优于ABA与2,4-D,也高于单独使用2,4-D,在2,4-D1.0 mg/L+KT0.5 mg/L时诱导率最大,达到93.45%;分化阶段6-BA0.1 mg/L与NAA 0.04-0.06 mg/L的激素组合中分化率差异不明显。

关键词: 白羊草, 真叶, 愈伤诱导, 植株再生

Abstract: The feasible hormone combinations were selected for callus induction and plantlet regeneration of Bothriochloa ischaemum(L.)Keng.using the 7-day old euphyllas of as explants,in order to build plamtlet regeneration system.The results show that the effect of KT was in evidence on callous induction,the interaction of KT and 2,4-D was superior than that of ABA and 2,4-D or 2,4-D only.The maximal callus induction rate reached 93.45% when the callus induction medium supplemented with 1.0 mg·L-1 2,4-D and 0.5 mg·L-1 KT.When the callus were transferred to the medium containing 0.1 mg·L-1 6-BA and 0.04-0.06 mg·L-1 NAA,the differentiation frequency of shoot was not significantly different.

Key words: Bothriochloa ischaemum(L.)Keng, Euphylla, Callus induction, Plant regeneration

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