NaCl胁迫对碱蒿活性氧产生及抗氧化酶活性的影响

doi:10.11733/j.issn.1007-0435.2010.06.014

›› 2010, Vol. 18 ›› Issue (6): 829-832.DOI: 10.11733/j.issn.1007-0435.2010.06.014

NaCl胁迫对碱蒿活性氧产生及抗氧化酶活性的影响

方志红, 董宽虎

山西农业大学动物科技学院, 山西, 太谷, 030801

收稿日期:2010-01-21 修回日期:2010-10-18 出版日期:2010-12-15 发布日期:2010-12-15
通讯作者: 董宽虎,E-mail:dongkuanhu@126.com
作者简介:方志红(1983- ),女,内蒙古赤峰市人,硕士研究生,研究方向为牧草抗逆性,E-mail:fangzhihong2007@126.com
基金资助:
“十一五”国家科技支撑计划课题(2007BAD56B01);农业部公益性行业(农业)科研专项(nyhyzx07-022);山西省科技攻关项目(20080312002-1)资助

Effects of NaCl Stress on Reactive Oxygen Species and Antioxidant Enzyme Activities of Artemisia anethifolia

FANG Zhi-hong, DONG Kuan-hu

College of Animal Science and Technolgy, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2010-01-21 Revised:2010-10-18 Online:2010-12-15 Published:2010-12-15

摘要/Abstract

摘要： 为了解NaCl胁迫对碱蒿(Artemisia anethifolia)耐盐性的影响,在温室盆栽条件下,通过研究碱蒿幼苗在不同浓度NaCl(0,25,50,100,200,400 mmol·L^-1)胁迫下的相对盐害率、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、脯氨酸(Pro)含量、H₂O₂含量和超氧阴离子(O₂^-)产生速率的变化,结果表明:随着NaCl浓度增加,碱蒿幼苗相对盐害率和H₂O₂含量增加,而SOD活性、POD活性、Pro含量、O₂^-产生速率和MDA含量均呈先升高后降低的变化趋势。这表明碱蒿具有较强的耐盐性,SOD活性、POD活性、MDA和Pro含量均可作为碱蒿的耐盐生理指标。

关键词: 碱蒿, 耐盐性, NaCl胁迫, 超氧化物歧化酶, 过氧化物酶

Abstract: This study reports the salt tolerance of Artemisia anethifolia regarding the change of the rate of salt injury,activity of superoxide dismutase(SOD) and peroxidase(POD),malondidehyde(MDA) content,free proline(Pro) content,H₂O₂ contents and superoxide radical(O₂^-) production rate in seedling of Artemisia anethifolia under different alkali concentration NaCl(0,25,50,100,200,400 mmol·L^-1) under greenhouse conditions.Results show that,with the increase of salt concentration,the seedling pre-integrated relative salt injury rates and H₂O₂ contents increased;SOD,POD,free proline,superoxide radical production rate and MDA contents of Artemisia anethifolia had low-high-low trend.We concluded that Artemisia anethifolia had a strong salt tolerance while SOD activity,POD activity,MDA content and free proline content could be used as important physiological indexes for salt tolerance in Artemisia anethifolia.

Key words: Artemisia anethifolia, Salt tolerance, NaCl stress, Superoxide dismutase, Peroxidase

中图分类号:

Q945.78

方志红, 董宽虎. NaCl胁迫对碱蒿活性氧产生及抗氧化酶活性的影响[J]. , 2010, 18(6): 829-832.

FANG Zhi-hong, DONG Kuan-hu. Effects of NaCl Stress on Reactive Oxygen Species and Antioxidant Enzyme Activities of Artemisia anethifolia[J]. , 2010, 18(6): 829-832.

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NaCl胁迫对碱蒿活性氧产生及抗氧化酶活性的影响

Effects of NaCl Stress on Reactive Oxygen Species and Antioxidant Enzyme Activities of Artemisia anethifolia

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