假俭草耐寒变异体的筛选及其生理鉴定

doi:10.11733/j.issn.1007-0435.2011.04.019

›› 2011, Vol. 19 ›› Issue (4): 652-656,662.DOI: 10.11733/j.issn.1007-0435.2011.04.019

假俭草耐寒变异体的筛选及其生理鉴定

刘明稀^1,2, 卢少云^2,3, 郭振飞^2,3

1. 湖南农业大学, 湖南长沙410128;华南农业大学生命科学学院, 广州510642;
2. 华南农业大学生命科学学院, 广州510642;
3. 华南农业大学广东省森林植物种质创新与利用重点实验室, 广州510642

收稿日期:2011-02-22 修回日期:2011-04-03 出版日期:2011-08-15 发布日期:2011-08-15
通讯作者: 郭振飞,E-mail:zhfguo@scau.edu.Cn
作者简介:刘明稀(1975- ),男,湖南新化人,讲师,博士,主要从事草坪草、牧草及能源草生物技术育种,E-mail:lmx751221@163.com
基金资助:
广东省科技计划项目(2006B20201035);湖南农业大学校青年基金(10QN30)项目资助

Chilling-tolerant Variants’ Screening and Physiological Identification of Centipedegrass

LIU Ming-xi^1,2, LU Shao-yun^2,3, GUO Zhen-fei^2,3

1. Hunan Agricultural University, Changsha, Hunan Province 410128, China;
2. College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong Province 518020, China;
3. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, Guangdong Province 510642, China

Received:2011-02-22 Revised:2011-04-03 Online:2011-08-15 Published:2011-08-15

摘要/Abstract

摘要： 对假俭草(Eremochloa ophiuroides(Munro)Hack.)胚性愈伤组织进行反复间歇低温处理并结合长期继代,诱导体细胞无性系变异的发生。通过人工气候箱低温筛选,在再生群体中获得5个耐寒性提高的变异植株。结果表明:低温处理前各耐寒变异植株及其对照植株间最大光化学效率(F_v/F_m)、相对电导率、丙二醛(MDA)含量、超氧物歧化酶(SOD)和过氧化氢酶(CAT)活性差异不显著。低温处理后所有植株的F_v/F_m均下降,相对电导率和MDA均升高,但耐寒变异体植株的F_v/F_m高于对照植株,相对电导率和MDA含量低于对照植株,表明耐寒植株受低温冷害程度较轻。SOD和CAT的活性在低温处理后均升高,但耐寒变异体植株的SOD和CAT活性均高于对照。因此,耐寒突变体的耐寒性与其SOD和CAT对低温的响应更敏感有关,并首次初步表明体细胞无性系变异技术在假俭草抗寒性改良育种中具有可行性。

关键词: 假俭草, 体细胞无性系变异, 耐寒性, 抗氧化酶

Abstract: Centipedegrass (Eremochloa ophiuroides (Munro) Hack.) is an important warm-season turfgrass species originating in China,however,its susceptibility to cold stress limited its practical application.In this paper,selection and identification of somaclonal variants of centipedegrass having an increased chilling tolerance were investigated.Embryogenic calli were subject to repeated interval cold-treatments in combination with long-term subcultures for induction of somaclonal variation.Five individual variants with improved chilling tolerance were selected and identified.Their physiological responses to chilling were further measured.There was no significant difference in maximal photochemistry efficiency of photosystem Ⅱ (F_v/F_m),ion leakage,malondialdehyde (MDA) content,and activities of superoxide dismutase (SOD) and catalase (CAT) between the five variants and their wild type control plants prior to chilling treatment.After chilling treatment at 6～8℃ for 20 d,all plants showed a decrease in F_v/F_m and increase in ion leakage and MDA content,while variants show higher levels of F_v/F_m and lower levels of ion leakage and MDA content than the control plants,indicating that less chilling injury occurred in the chilling-tolerant variants.Chilling treatment increased activities of SOD and CAT in all plants,indicating a response of antioxidant enzymes to chilling in centipedegrass,while there were higher activities of SOD and CAT in testedvariants than the control plants.This suggests that the increased chilling tolerance in the five variants is associated with the enhanced up-regulated activities of SOD and CAT.Further,results suggest the technology of somaclonal variation could play an important role in the improving chilling tolerance of centipedegrass.

Key words: Centipedegrass, Somaclonal variation, Chilling tolerance, Antioxidant enzymes

中图分类号:

Q319.2

刘明稀, 卢少云, 郭振飞. 假俭草耐寒变异体的筛选及其生理鉴定[J]. , 2011, 19(4): 652-656,662.

LIU Ming-xi, LU Shao-yun, GUO Zhen-fei. Chilling-tolerant Variants’ Screening and Physiological Identification of Centipedegrass[J]. , 2011, 19(4): 652-656,662.

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假俭草耐寒变异体的筛选及其生理鉴定

Chilling-tolerant Variants’ Screening and Physiological Identification of Centipedegrass

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