NaCl胁迫下紫花苜蓿幼苗抗氧化酶活性的研究

doi:10.11733/j.issn.1007-0435.2012.01.014

›› 2012, Vol. 20 ›› Issue (1): 83-87.DOI: 10.11733/j.issn.1007-0435.2012.01.014

NaCl胁迫下紫花苜蓿幼苗抗氧化酶活性的研究

龙明秀¹, 许岳飞¹, 何学青¹, 廖允成²

1. 西北农林科技大学动物科技学院, 陕西杨凌712100;
2. 西北农林科技大学农学院, 陕西杨凌712100

收稿日期:2011-08-09 修回日期:2011-11-11 出版日期:2012-02-15 发布日期:2012-07-06
作者简介:龙明秀(1971- ),女,陕西宁强人,副教授,博士研究生,主要从事牧草栽培生理研究,E-mail:longmingxiu@nwsuaf.edu.cn
基金资助:
国家科技支撑计划项目(2011BAD17B05);国家公益性行业(农业)科技项目(200903060);西北农林科技大学唐仲英育种基金(A212020901);陕西省农业攻关项目(2011K01-06)资助

Antioxidant Enzymes Activities of Medicago sativa L.Seedling under Salt Stress

LONG Ming-xiu¹, XU Yue-fei¹, HE Xue-qing¹, LIAO Yun-cheng²

1. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Provnice 712100, China;
2. College of Agronomy, Northwest A&F University, Yangling, Shaanxi Province 712100, China

Received:2011-08-09 Revised:2011-11-11 Online:2012-02-15 Published:2012-07-06

摘要/Abstract

摘要： 以紫花苜蓿品种金皇后(Medicago sativa L.‘Golden Empress’)为试验材料,用不同浓度NaCl(0,40,80,120和160mmol·L^-1)对其幼苗进行胁迫处理,分别对叶片和根部的超氧阴离子自由基(O₂^·-)、丙二醛(MDA)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸(Vc)和总抗氧化能力(T-AOC)等指标进行测定,旨在揭示紫花苜蓿幼苗地上、地下部分对盐胁迫的反应及抗氧化防御机制,为苜蓿耐盐性评价及新品种选育提供理论依据。结果表明:随着NaCl盐浓度增加,苜蓿根中O₂^·-和Vc先增加后减少,MDA含量和POD活性增加,SOD,CAT和T-AOC活性减少;苜蓿叶片中O₂^·-先增加后减少,MDA,Vc含量和SOD活性增加,POD,CAT和T-AOC活性减少。综合表明,相对于地上部分来说,盐对苜蓿幼苗根系的伤害更大。

关键词: 紫花苜蓿, 盐胁迫, 抗氧化作用, 调节机制

Abstract: To explore the responses of alfalfa(Medicago sativa L.’Golden Empress’) to salt stress,provide the scientific evaluation of alfalfa salt tolerance,antioxidant defense mechanisms of alfalfa seedling were treated.The antioxidant enzymes activities(Superoxide,MDA,POD,SOD,CAT,Vc and T-AOC) in alfalfa leaves and roots under different NaCl stress(0,40,80,120 and 160 mmol·L^-1) were measured.Results showed that both superoxide and Vc levels initially increased then decreased while MDA and POD increased but SOD,CAT and T-AOC decreased in alfalfa root.However,superoxide levels initially increased then decreased,MDA,Vc and SOD increased,POD,CAT and T-AOC decreased in leaves of alfalfa under different NaCl stress.Comprehensive analysis showed that the roots of alfalfa seedlings experienced more serious salt damage compared to aboveground parts.

Key words: Alfalfa, Salt stress, Antioxidant, Regulation mechanism

中图分类号:

Q945.78

龙明秀, 许岳飞, 何学青, 廖允成. NaCl胁迫下紫花苜蓿幼苗抗氧化酶活性的研究[J]. , 2012, 20(1): 83-87.

LONG Ming-xiu, XU Yue-fei, HE Xue-qing, LIAO Yun-cheng. Antioxidant Enzymes Activities of Medicago sativa L.Seedling under Salt Stress[J]. , 2012, 20(1): 83-87.

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NaCl胁迫下紫花苜蓿幼苗抗氧化酶活性的研究

Antioxidant Enzymes Activities of Medicago sativa L.Seedling under Salt Stress

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