草地学报

草地学报 ›› 2014, Vol. 22 ›› Issue (3): 673-676.DOI: 10.11733/j.issn.1007-0435.2014.03.037

• 研究专报 • 上一篇    

柳枝稷基因枪转化法中国产裂解膜代替进口膜的可行性研究

徐开杰1, 史丽丽2, 奚亚军1, 朱建楚1   

  1. 1. 西北农林科技大学农学院, 陕西 杨凌 712100;
    2. 邯郸农业科学院, 河北 邯郸 056001
  • 收稿日期:2013-01-20 修回日期:2014-03-10 发布日期:2014-06-04
  • 通讯作者: 奚亚军，朱建楚
  • 作者简介:徐开杰（1981-），男，山东日照人，博士研究生，主要从事植物基因工程研究，E-mail：xukaijie2010@126.com
  • 基金资助:

    西北农林科技大学大型仪器设备新功能开发项目“PDS-1000/he基因枪耗材国产化研究”(dysb110104)；国家自然科学基金(31171607)(31371690)资助

Feasibility Study on Domestic Rupture Disk Replaced Imported One in Particle Bombardment

XU Kai-jie1, SHI Li-li2, XI Ya-jun1, ZHU Jian-chu1   

  1. 1. Northwest A&F University, Yangling, Shaanxi Province 712100, China;
    2. Handan Agriculture Academy of Sciences, Handan, Hebei Province 056001, China
  • Received:2013-01-20 Revised:2014-03-10 Published:2014-06-04

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摘要：

利用国产和进口裂解膜转化柳枝稷(Panicum virgatum L.)愈伤组织，对裂解膜裂解时的压强、抗性愈伤率、分化再生苗及阳性转化率进行统计分析。结果表明：国产裂解膜在裂解时压强的稳定性略低于进口膜，但其平均值差异不显著；除抗性愈伤率国产裂解膜显著低于进口膜外(P<0.05)，其余各指标差异均不显著。因此，在柳枝稷基因枪转化法中国产裂解膜完全可以代替进口膜，节约试验经费。

关键词: 柳枝稷, 基因枪转化法, 裂解膜, 潮霉素抗性愈伤, 再生植株

Abstract:

Comparing domestic rupture disk with imported one in the particle bombardment of switchgrass calluses, the burst pressure of rupture disk, the rate of hyg-resistance callus, the number of regeneration plants and the rate of transgenic plants were analyzed. The results showed that the stability of domestic rupture disk was poorer than that of imported one, whereas the average pressures of both disks were not significant difference. All tested indexes except the rate of resistant callus were also no significant difference between domestic rupture disks and imported ones. In conclusion, the domestic rupture disk could completely replace imported and these results could provide a theoretical premise to save scientific research funds in the particle bombardment.

Key words: Switchgrass, Particle bombardment, Rupture disk, Hyg-resistant calluses, Regeneration plants

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