柳枝稷PvbZIP8基因的克隆与表达分析

doi:10.11733/j.issn.1007-0435.2019.03.006

草地学报 ›› 2019, Vol. 27 ›› Issue (3): 560-566.DOI: 10.11733/j.issn.1007-0435.2019.03.006

柳枝稷PvbZIP8基因的克隆与表达分析

王伟伟, 王勇锋, 张舒梦, 王竹林, 孙风丽, 张超, 奚亚军

西北农林科技大学农学院, 陕西杨凌 712100

收稿日期:2019-02-26 修回日期:2019-05-14 出版日期:2019-06-15 发布日期:2019-07-19
通讯作者: 奚亚军
作者简介:王伟伟(1992-),男,河南驻马店人,硕士研究生,主要从事柳枝稷分子抗逆性研究,E-mail:wangweiw22@126.com
基金资助:
国家自然科学基金（31371690）资助

Cloning and Expression Analysis of Switchgrass PvbZIP8 Gene

WANG Wei-wei, WANG Yong-feng, ZHANG Shu-meng, WANG Zhu-lin, SUN Feng-li, ZHANG Chao, XI Ya-jun

College of Agronomy, Northwest A & F University, Yangling, Shaanxi Province 712100, China

Received:2019-02-26 Revised:2019-05-14 Online:2019-06-15 Published:2019-07-19

摘要/Abstract

摘要： bZIP转录因子在植物非生物逆境胁迫的响应中发挥着重要作用，本研究通过同源克隆在柳枝稷（Panicum virgatum L.）中获得PvbZIP8基因，并对该基因进行了初步的生物信息学分析，同时利用荧光定量PCR技术进行了非生物胁迫下基因表达模式分析以及组织特异性表达分析。结果显示：基因的开放阅读框长度为468 bp，编码155个氨基酸，分子式为C₇₆₁H₁₂₄₈N₂₄O₂₃S₈，分子量为17.81 kDa，为亲水性蛋白。系统进化分析表明该蛋白与哈氏黍（Panicum hallii）、谷子（Setaria italica）和糜子（Panicum miliaceum）相似性较高，并且具有典型的bZIP保守结构域，属于bZIP转录因子家族成员。定量PCR结果显示，PvbZIP8基因在盐、干旱、高温和低温胁迫下上调表达，在柳枝稷抗逆过程中发生作用。组织特异性表达分析表明，PvbZIP8在多个组织或器官均有表达，其中在根、茎、叶中表达量较高。本研究初步确定柳枝稷PvbZIP8基因响应抗逆性反应，并为进一步研究柳枝稷PvbZIP8基因的生物学功能奠定基础。

关键词: 柳枝稷(Panicum virgatum L.), bZIP转录因子, 基因克隆, 非生物胁迫, 表达分析

Abstract: bZIP transcription factors play significant roles in regulating expression of functional genes in response to growth and development as well as environmental stress signaling. In this paper,the PvbZIP8 gene was obtained by homologous cloning from switchgrass (Panicum virgatum L.). Subsequently,the bioinformatics analysis,tissue-specific expression analysis and gene expression patterns analysis under abiotic stress were carried out. The results showed that the length of open reading frame of PvbZIP8 gene was 468bp,encoding 155 amino acids. In addition,PvbZIP8 was predicted as a hydrophilic protein,and the molecular formula was C₇₆₁H₁₂₄₈N₂₄O₂₃S₈ and the molecular weight was 17.81 kDa. Phylogenetic analysis indicated that the protein sequence was highly similar to Panicum hallii,Setaria italica and Panicum miliaceum,and it contained the typical conserved bZIP domain and belonged to the bZIP transcription factor family. Quantitative PCR results showed that PvbZIP8 gene was up-regulated under salt,drought,high temperature and low temperature stress,indicating key roles in response to abiotic stress in switchgrass. Tissue-specific expression analysis showed that PvbZIP8 was detected in different tissues and organs,with significantly high expressions in roots,stems and leaves. The investigation determined PvbZIP8 gene was related to abiotic stress tolerance and laid the foundation for further study of PvbZIP8 gene function.

Key words: Switchgrass, bZIP transcription factor, Gene cloning, Abiotic stress, Expression analysis

中图分类号:

S543+.9

王伟伟, 王勇锋, 张舒梦, 王竹林, 孙风丽, 张超, 奚亚军. 柳枝稷PvbZIP8基因的克隆与表达分析[J]. 草地学报, 2019, 27(3): 560-566.

WANG Wei-wei, WANG Yong-feng, ZHANG Shu-meng, WANG Zhu-lin, SUN Feng-li, ZHANG Chao, XI Ya-jun. Cloning and Expression Analysis of Switchgrass PvbZIP8 Gene[J]. Acta Agrestia Sinica, 2019, 27(3): 560-566.

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柳枝稷PvbZIP8基因的克隆与表达分析

Cloning and Expression Analysis of Switchgrass PvbZIP8 Gene

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