紫花苜蓿CNGC基因家族成员鉴定及分析

doi:10.11733/j.issn.1007-0435.2024.02.028

草地学报 ›› 2024, Vol. 32 ›› Issue (2): 588-598.DOI: 10.11733/j.issn.1007-0435.2024.02.028

紫花苜蓿CNGC基因家族成员鉴定及分析

李小红¹, 王晓彤¹, 麻旭霞¹, 蔡文祺¹, 冯学丽¹, 马梦凡¹, 李淑霞^1,2,3

1. 宁夏大学林业与草业学院, 宁夏银川 750021;
2. 宁夏草牧业工程技术研究中心, 宁夏银川 750021;
3. 农业农村部饲草高效生产模式创新重点实验室, 宁夏银川 750021

收稿日期:2023-09-26 修回日期:2023-11-16 出版日期:2024-02-15 发布日期:2024-03-06
通讯作者: 李淑霞,E-mail:lishuxia620@163.com
作者简介:李小红(1996-),男,回族,宁夏固原人,硕士研究生,主要从事牧草抗逆育种研究,E-mail:lxh0895@163.com
基金资助:
国家自然科学基金项目（32101426）；宁夏自然科学基金项目（2023AAC05019）资助

Identification and Analysis of CNGC Gene Family Members in Alfalfa

LI Xiao-hong¹, WANG Xiao-tong¹, MA Xu-xia¹, CAI Wen-qi¹, FENG Xue-li¹, MA Meng-fan¹, LI Shu-xia^1,2,3

1. College of Forestry and Prataculture, Ningxia University, Yinchuan, Ningxia 750021, China;
2. Ningxia Grassland and Animal Husbandry Engineering Technology Research Center, Yinchuan, Ningxia 750021, China;
3. Key Laboratory for Model Innovation in Forage Production Efficiency, Ministry of Agriculture and Rural Affairs, Yinchuan, Ningxia 750021, China

Received:2023-09-26 Revised:2023-11-16 Online:2024-02-15 Published:2024-03-06

摘要/Abstract

摘要： 环核苷酸门控通道（CNGC）基因家族作为非选择性阳离子通道基因家族之一，在植物信号转导、生长发育和环境胁迫等生理过程中发挥着重要作用。本研究利用生物信息学手段和转录组数据对紫花苜蓿CNGC家族成员的进化关系、基因结构、保守基序、顺式作用元件、染色体定位、共线性关系以及基因表达进行分析。结果表明，紫花苜蓿MsCNGC基因家族成员有16个，在染色体上不均匀分布，存在片段重复，大多数蛋白定位于细胞质膜。系统发育树将MsCNGCs为4个亚家族。基因结构和保守基序分析表明，都含有cNMP/CNBD和ITP功能域。顺式作用元件分析表明，MsCNGCs基因含有许多与非生物或生物胁迫和激素响应元件。MsCNGC4和MsCNGC7.2蛋白之间存在互作。MsCNGC基因具有组织表达特异性。MsCNGC基因对干旱、盐和激素等非生物胁迫均有显著的响应，为进一步研究MsCNGC基因在调控非生物胁迫过程中的潜在功能提供了理论依据。

关键词: 紫花苜蓿, CNGC基因家族, 全基因组, 系统进化, 基因结构, 非生物胁迫

Abstract: The cyclic nucleotide-gated channels (CNGCs) gene family functions as one of the non-selective cation channel gene families and plays important roles in physiological processes such as plant signal transduction,growth and development,and environmental stress. In this study,we analyzed the evolutionary relationship,gene structure,conserved motifs,cis-acting elements,chromosome localization,collinearity,and gene expression patterns of the CNGC family members in alfalfa using bioinformatic tools and transcriptomic data. The results showed that there were 16 members of MsCNGC genes family in alfalfa,which were unevenly distributed on chromosomes,with segmental repeation,and most of proteins were located in the plasma membrane. The phylogenetic tree divided MsCNGCs into four subfamilies. Gene structure and conserved motif analysis showed that cNMP/CNBD and ITP functional domains were present. Cis-acting element analysis showed that MsCNGC genes contained many elements that responded to abiotic or biological stresses and hormones. There was interaction between MsCNGC4 and MsCNGC7.2 proteins. MsCNGC genes had tissue specificity. MsCNGC genes had significant response to drought,salt and hormone stress,which provided a theoretical basis for further research on the potential function of MsCNGC genes in the regulation of abiotic stress.

Key words: Alfalfa, CNGC genes family, Genome-wide, Systematic evolution, Gene structure, Abiotic stress

中图分类号:

S512.6

李小红, 王晓彤, 麻旭霞, 蔡文祺, 冯学丽, 马梦凡, 李淑霞. 紫花苜蓿CNGC基因家族成员鉴定及分析[J]. 草地学报, 2024, 32(2): 588-598.

LI Xiao-hong, WANG Xiao-tong, MA Xu-xia, CAI Wen-qi, FENG Xue-li, MA Meng-fan, LI Shu-xia. Identification and Analysis of CNGC Gene Family Members in Alfalfa[J]. Acta Agrestia Sinica, 2024, 32(2): 588-598.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2024.02.028

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2024/V32/I2/588

参考文献

[1] WU M,LI Y,CHEN D,LIU H,et al. Genome-wide identification and expression analysis of the IQD gene family in moso bamboo (Phyllostachys edulis)[J]. Scientific Reports, 2016,(6):24520
[2] SAAND M A,XU Y P,MUNYAMPUNDU J P,et al. Phylogeny and evolution of plant cyclic nucleotide-gated ion channel (CNGC) gene family and functional analyses of tomato CNGCs[J]. DNA Research, 2015,22(6):471-483
[3] VIRDI A S,SINGH S,SINGH P. Abiotic stress responses in plants:roles of calmodulin-regulated proteins[J]. Frontiers in Plant Science, 2015(6):809
[4] DU L,ALI G S,SIMONS K A,et al. Ca ⁽²⁺⁾/calmodulin regulates salicylic-acid-mediated plant immunity[J]. Nature, 2009,457(7233):1154-1158
[5] CHEN L,WANG W,HE H,et al. Genome-wide identification,characterization and experimental expression analysis of cngc gene family in gossypium[J]. International Journal of Molecular Sciences, 2023,24(5):4617
[6] CHRISTOPHER D A,BORSICS T,YUEN C Y,et al. The cyclic nucleotide gated cation channel AtCNGC10 traffics from the ER via Golgi vesicles to the plasma membrane of Arabidopsis root and leaf cells[J]. BMC Plant Biology, 2007(7):1-13
[7] YUEN C C Y,CHRISTOPHER D A. The group IV-A cyclic nucleotide-gated channels,CNGC19 and CNGC20,localize to the vacuole membrane in Arabidopsis thaliana[J]. AOB Plants, 2013(5):plt012
[8] CHARPENTIER M,SUN J,VAZ MARTINS T,et al. Nuclear-localized cyclic nucleotide-gated channels mediate symbiotic calcium oscillations[J]. Science, 2016,352(6289):1102-1105
[9] KAUPP U B,SEIFERT R. Cyclic nucleotide-gated ion channels[J]. Physiological Reviews, 2002,82(3):769-824
[10] MA W,Q I Z,SMIGEL A,et al. Ca²⁺,cAMP,and transduction of non-self-perception during plant immune responses[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009,106(49):20995-21000
[11] ZHOU L,LAN W,JIANG Y,et al. A calcium-dependent protein kinase interacts with and activates a calcium channel to regulate pollen tube growth[J]. Molecular Plant, 2014,7(2):369-376
[12] 刘文豪,田琴,余渝,等. 陆地棉CNGC全基因组鉴定及表达分析[J].江苏农业科学,2021,49(24):49-56
[13] 王晓宇,张洪雨,刘栩铭,等.蓖麻CNGC基因家族鉴定与冷胁迫下的表达模式分析[J].分子植物育种,2021,19(22):7373-7382
[14] DEFALCO T A,MARSHALL C B,MUNRO K,et al. Multiple calmodulin-binding sites positively and negatively regulate Arabidopsis cyclic nucleotide-gated channel12[J]. Plant Cell, 2016,28(7):1738-1751
[15] GU L L,GAO Q F,WANG Y F. Cyclic nucleotide-gated channel 18 functions as an essential Ca²⁺ channel for pollen germination and pollen tube growth in Arabidopsis[J]. Plant Signaling & Behavior, 2017,12(11):e1197999
[16] GUO K M,BABOURINA O,CHRISTOPHER D A,et al. The cyclic nucleotide-gated channel,AtCNGC10,influences salt tolerance in Arabidopsis[J]. Physiology Plant, 2008,134(3):499-507
[17] ORANAB S,GHAFFAR A,KIRAN S,et al. Molecular characterization and expression of cyclic nucleotide gated ion channels 19 and 20 in Arabidopsis thaliana for their potential role in salt stress[J]. Saudi Journal of Biological Sciences, 2021,28(10):5800-5807
[18] FRIETSCH S,WANG Y F,SLADEK C,et al. A cyclic nucleotide-gated channel is essential for polarized tip growth of pollen[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007,104(36):14531-14536
[19] KUGLER A,KÖHLER B,PALME K, et al. Salt-dependent regulation of a CNGC channel subfamily in Arabidopsis[J]. BMC Plant Biology, 2009,9(1):1-11
[20] SAAND M A,XU Y P,LI W,et al. Cyclic nucleotide gated channel gene family in tomato:genome-wide identification and functional analyses in disease resistance[J]. Frontiers in Plant Science, 2015(6):303
[21] ZHANG Y,LI Y,YANG J,et al. Genome-wide analysis and expression of cyclic nucleotide-gated ion channel (CNGC) family genes under cold stress in Mango (Mangifera indica)[J]. Plants (Basel), 2023,12(3):592
[22] LU Z,YIN G,CHAI M,et al. Systematic analysis of CNGCs in cotton and the positive role of GhCNGC32 and GhCNGC35 in salt tolerance[J]. BMC Genomics, 2022,23(1):560
[23] 李家兴,周丽莹,苑峰,等.紫花苜蓿MsHB1基因的克隆及表达分析[J].草地学报,2023,31(12):3617-3625
[24] 唐芳,梅亭,高佳荷,等.紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析[J].草地学报,2023,31(9):2608-2620
[25] ZELMAN A K,DAWE A,GEHRING C,et al. Evolutionary and structural perspectives of plant cyclic nucleotide-gated cation channels[J]. Frontiers in Plant Science,2012(3):95
[26] XU G,GUO C,SHAN H,et al. Divergence of duplicate genes in exon-intron structure[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012,109(4):1187-1192
[27] HAO L,QIAO X. Genome-wide identification and analysis of the CNGC gene family in maize[J]. Peer J, 2018(6):e5816
[28] NAWAZ Z,KAKAR K U,SAAND M A,et al. Cyclic nucleotide-gated ion channel gene family in rice,identification,characterization and experimental analysis of expression response to plant hormones,biotic and abiotic stresses[J]. BMC Genomics, 2014,15(1):853
[29] GUO J,ISLAM M,LIN H,et al. Genome-wide identification of cyclic nucleotide-gated ion channel gene family in wheat and functional analyses of TaCNGC14 and TaCNGC16[J]. Frontiers in Plant Science, 2018(9):18
[30] CHEN J,WANG B,CHUNG J S,et al. The role of promoter cis-element,mRNA capping,and ROS in the repression and salt-inducible expression of AtSOT12 in Arabidopsis[J]. Frontiers in Plant Science, 2015(6):974
[31] WANG L,JIA G,JIANG X,et al. Altered chromatin architecture and gene expression during polyploidization and domestication of soybean[J]. Plant Cell, 2021,33(5):1430-1446
[32] SAAND M A,XU Y P,MUNYAMPUNDU J P,et al. Phylogeny and evolution of plant cyclic nucleotide-gated ion channel (CNGC) gene family and functional analyses of tomato CNGCs[J]. DNA Research, 2015,22(6):471-483
[33] MAGADUM S,BANERJEE U,MURUGAN P,et al. Gene duplication as a major force in evolution[J]. Journal of Genetics, 2013,92(1):155-161
[34] CUI Y,WANG J,BAI Y,et al. Identification of CNGCs in glycine max and screening of related resistance genes after fusarium solani infection[J]. Biology,2023,12(3):439
[35] 刘正林,庄南生,王英,等.巴西橡胶树HbWRKY基因家族10个成员在染色体上的定位[J].植物遗传资源学报,2018,19(6):1170-1179

紫花苜蓿CNGC基因家族成员鉴定及分析

Identification and Analysis of CNGC Gene Family Members in Alfalfa

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	景芳, 师尚礼, 南攀, 马瑞宏, 阿芸, 陆保福, 关键, 张辉辉. 不同苜蓿品种叶片特征、光合生理特性与产量性状的比较[J]. 草地学报, 2024, 32(2): 369-377.
[2]	刘婧禹, 赵海君, 欧成明, 贾志程, 毛培胜. 褪黑素引发对Na₂SO₄胁迫下紫花苜蓿种子发芽特性的影响[J]. 草地学报, 2024, 32(2): 378-385.
[3]	李启娇, 崔东毅, 段新慧, 刘莉, 许文花, 李庆玺, 郑立文, 任健, 朱丽敏, 马向丽. 褪黑素浸种对铝胁迫下苜蓿种子萌发及幼苗抗氧化生理的影响[J]. 草地学报, 2024, 32(2): 535-542.
[4]	巩泽琳, 额尔德木图, 苏小港, 成蓉蓉, 杨鹤明, 张晓琳, 翟鹏辉. 水肥添加对草田轮作系统土壤呼吸速率的影响[J]. 草地学报, 2024, 32(2): 562-569.
[5]	吴倩倩, 苏娅, 曾祥明, 李栋恒, 程巍, 郝俊. 紫花苜蓿、白三叶硒蛋白的提取方法筛选及工艺优化[J]. 草地学报, 2024, 32(2): 654-660.
[6]	郭美琪, 郭童天, 徐民乐, 张英俊. 补播苜蓿对退化草地植物群落结构和生产力的影响途径[J]. 草地学报, 2024, 32(1): 46-53.
[7]	李心, 武敬也, 陈菲儿, 樊璐, 马琳, 王学敏. MsWRKY33转录调控MsACS2影响紫花苜蓿耐盐性的研究[J]. 草地学报, 2024, 32(1): 54-65.
[8]	张玲, 麻冬梅, 刘晓霞, 马巧利, 侯汶君, 李嘉文, 苏立娜, 杭嘉慧. 根灌外源褪黑素对干旱胁迫下紫花苜蓿生理特性的影响研究[J]. 草地学报, 2024, 32(1): 198-206.
[9]	朱敏, 牛帅帅, 侯青青, 杨轩, 徐洪雨. 山西晋中盆地紫花苜蓿生产对水分和气温的响应[J]. 草地学报, 2024, 32(1): 207-218.
[10]	陈海雁, 黄荣, 赵亮, 杨杰, 谢金晶, 张振粉. Cp2-EPS对盐胁迫下紫花苜蓿苗期形态及光合特性的影响[J]. 草地学报, 2024, 32(1): 229-238.
[11]	李青璞, 白建海, 姚拓, 雷杨, 周泽, 张琛, 付卫刚. 微生物菌剂与氮肥配施对紫花苜蓿生长及土壤性质的影响[J]. 草地学报, 2024, 32(1): 314-321.
[12]	武玲玲, 谢永丽, 杨杰, 杨雪, 李俊熹, 王添, 高英, 常斐斐. 萎缩芽孢杆菌WL520生物活性及干旱胁迫下促紫花苜蓿生长效应[J]. 草地学报, 2024, 32(1): 322-330.
[13]	张昭, 聂宇婷, 崔凯伦, 吕艳贞, 闫慧芳. 褪黑素调控草类植物生长发育及抗逆性功能研究进展[J]. 草地学报, 2023, 31(9): 2571-2581.
[14]	唐芳, 梅亭, 高佳荷, 王佳妮, 石凤翎, 高翠萍. 紫花苜蓿GPAT基因家族鉴定及在盐碱胁迫下的表达模式分析[J]. 草地学报, 2023, 31(9): 2608-2620.
[15]	李乐, 李丹宁, 杨叶研, 潘新雅, 万懿琦, 张国云, 席杰军, 王亚芳, 杨培志. 干旱胁迫下紫花苜蓿叶片代谢组分析及气孔调节物质的筛选[J]. 草地学报, 2023, 31(9): 2671-2683.