草地学报 ›› 2014, Vol. 22 ›› Issue (4): 803-807.DOI: 10.11733/j.issn.1007-0435.2014.04.019

• 研究论文 • 上一篇    下一篇

中国芒属植物抗寒性初步研究

杜希夷1, 肖亮2, 蒋建雄1, 易自力1, 陈智勇1, 黄红梅1, 覃静萍1, 刘清波1   

  1. 1. 湖南农业大学生物科学技术学院, 湖南 长沙 410128;
    2. 湖南农业大学 作物种质和资源利用国家重点实验室培育基地, 湖南 长沙 410128
  • 收稿日期:2013-10-03 修回日期:2013-12-02 出版日期:2014-08-15 发布日期:2014-08-04
  • 通讯作者: 易自力
  • 作者简介:杜希夷(1986-),女,湖南永州人,硕士研究生,主要从事芒属植物抗寒机理研究,E-mail:251402642@qq.com
  • 基金资助:

    湖南省自然科学基金(12JJ2019);国家科技支撑计划(2013BAD22B02)资助

Study on the Cold Resistance of Miscanthus Andersson in China

DU Xi-yi1, XIAO Liang2, JIANG Jian-xiong1, YI Zi-li1, CHEN Zhi-yong1, HUANG Hong-mei1, QIN Jing-ping1, LIU Qing-bo1   

  1. 1. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan Province 410128, China;
    2. Key Laboratory of Group Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, Hunan Province 410128, China
  • Received:2013-10-03 Revised:2013-12-02 Online:2014-08-15 Published:2014-08-04

摘要:

以13份具有地理代表性的芒属(Miscanthus Andersson)植物为材料,分别以5,0,-5,-10,-15,-20和-25℃共7个温度梯度进行冷冻胁迫,通过电导法测定电解质外渗率来评价各材料的抗寒性,并对供试材料半致死温度(LT50)与其原产地地理因子进行回归分析。结果表明:半致死温度(LT50)的变化范围为-2.92~-23.85℃,抗寒性由强到弱依次为C0429(-23.85℃) >D0341(-14.10℃) >B0534(-9.60℃) >B0121(-6.97℃) >A0502(-6.77℃) >A0110(-6.51℃) >B0120(-6.34℃) >B0605(-5.39℃) >A0107(-5.00℃) >C0517(-4.27℃) >B0145(-3.26℃) >C0128(-3.08℃) >C0129(-2.92℃),回归分析表明,半致死温度(LT50)与纬度和海拔成极显著负相关,说明芒属植物抗寒性与地理因子具有一定的相关性。研究结果将为芒属植物抗寒种质资源的筛选提供理论基础。

关键词: 芒属, 抗寒性, 电导率, Logistic方程

Abstract:

The leaves of thirteen Miscanthus Andersson accessions were taken as tested materials to study the cold resistances of them by the means of electrical conductivity method. Seven temperature gradients (5, 0,-5,-10,-15,-20,-25℃) were designed as freezing stresses. The line regressive relationships of LT50 with latitude and altitude were analyzed. The results showed that the variation range of LT50 was-2.92~-23.85 ℃, The cold resistance of tested samples ranked as C0429(-23.85℃) >D0341(-14.10℃) >B0534(-9.60℃) >B0121(-6.97℃) >A0502(-6.77℃) >A0110(-6.51℃) >B0120(-6.34℃) >B0605(-5.39℃) >A0107(-5.00℃) >C0517(-4.27℃) >B1045(-3.26℃) >C0128(-3.08℃) >C0129(-2.92℃). Regression analyses indicated that there were significant relationships between LT50 and latitude or altitude. These results provide a theoretical basis for the cold resistance screening of Miscanthus germplasm resource in China.

Key words: Miscanthus, Cold resistance, Electrical conductivity, Logistic equation

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