紫花苜蓿MsSOS1基因的克隆与表达分析

doi:10.11733/j.issn.1007-0435.2018.06.028

草地学报 ›› 2018, Vol. 26 ›› Issue (6): 1479-1489.DOI: 10.11733/j.issn.1007-0435.2018.06.028

紫花苜蓿MsSOS1基因的克隆与表达分析

方志红¹, 崔苗苗², 张燕¹, 任彬琳¹, 石永红¹, 刘建宁¹, 王运琦¹, 董宽虎³, 高洪文², 吴欣明¹

1. 山西省农业科学院畜牧兽医研究所, 山西太原 030032;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
3. 山西农业大学动物科技学院, 山西太谷 030801

收稿日期:2018-08-06 修回日期:2018-11-23 出版日期:2018-12-15 发布日期:2019-01-28
通讯作者: 吴欣明
作者简介:方志红(1983-),女,内蒙古赤峰人,博士,助理研究员,E-mail:fangzhihong2007@126.com
基金资助:
国家国际科技合作（R）专项；山西省农业科学院育种工程（17yzgc113）；中国农科院科技创新工程（ASTIP-IAS10）；现代农业产业技术体系专项资金（CARS-35-25）；山西省农业科学院所长青年引导专项项目（yydzx04）资助

Cloning and Expression Analysis of MsSOS1 gene from Medicago sativa

FANG Zhi-hong¹, Cui Miao-miao², ZHANG Yan¹, REN Bin-lin¹, SHI Yong-hong¹, LIU Jian-ning¹, WANG Yun-qi¹, DONG Kuan-hu³, GAO Hong-wen², WU Xin-ming¹

1. Animal Husbandry and Veterinary Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi Province 030032, China;
2. Institute of Aaimal Science, Chinese Academy of Agricultures Sciences, Beijing 100193, China;
3. College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2018-08-06 Revised:2018-11-23 Online:2018-12-15 Published:2019-01-28

摘要/Abstract

摘要： Na⁺/H⁺逆向转运蛋白基因SOS1（salt overly sensitive 1）是植物在抵御盐胁迫过程中一个重要的必需基因之一。本研究在紫花苜蓿（Medicago sativa）叶片中克隆得到一个MsSOS1基因，编码859个氨基酸，具有一个CAP-ED superfamily结构域、一个Crp superfamily结构域和一个Na⁺/H⁺ Exchanger superfamily结构域。与鹰嘴豆、大豆、羽扇豆、花生和葡萄的一致性分别是91%，87%，85%，84%和77%。实时荧光定量PCR分析表明，MsSOS1基因在根、茎、叶和花中均有表达，其中在根中的表达量最高，花中最低。此外，MsSOS1基因在4℃、PEG和ABA的胁迫中的表达均受到调控，推测该基因的表达与紫花苜蓿的抗逆性有关。

关键词: 紫花苜蓿, MsSOS1, 逆境胁迫, 表达分析

Abstract: Salt overly sensitive 1 (SOS1) gene is one of the essential genes in the biological process of plant against salt stress. In this study,a MsSOS1 gene was cloned from Medicago sativa leaves. It encodes 859 amino acids,has one conserved CAP-ED superfamily,one conserved Crp superfamily and one conserved Na⁺/H⁺ Exchanger superfamily. MsSOS1 has identity of 91%,87%,85%,84% and 77% of SOS1 gene from Cicer arietinum,Glycine max,Lupinus angustifolius,Arachis ipaensis and Vitis vinifera, respectively. Real-time quantitative PCR analysis revealed that MsSOS1 was expressed in root,stem,leaf and flower of Medicago sativa,with the highest expression in root and the lowest in flower. Besides,the expression of MsSOS1 gene could be regulated by the treatments of 4℃,PEG and ABA. This paper suggested that MsSOS1 involves in Medicago sativa stress tolerance.

Key words: Medicago sativa, MsSOS1, Adversity stress, Expression analysis

中图分类号:

S963.22+3.3

方志红, 崔苗苗, 张燕, 任彬琳, 石永红, 刘建宁, 王运琦, 董宽虎, 高洪文, 吴欣明. 紫花苜蓿MsSOS1基因的克隆与表达分析[J]. 草地学报, 2018, 26(6): 1479-1489.

FANG Zhi-hong, Cui Miao-miao, ZHANG Yan, REN Bin-lin, SHI Yong-hong, LIU Jian-ning, WANG Yun-qi, DONG Kuan-hu, GAO Hong-wen, WU Xin-ming. Cloning and Expression Analysis of MsSOS1 gene from Medicago sativa[J]. Acta Agrestia Sinica, 2018, 26(6): 1479-1489.

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参考文献

[1] Li D,Zhang Y,Hu X,et al. Transcriptional profiling of Medicago truncatula under salt stress identified a novel CBF transcription factor MtCBF4 that plays an important role in abiotic stress responses[J]. BMC Plant Biology,2011,11(1):109-128
[2] Munns R,Tester M. Mechanisms of Salinity Tolerance[J]. Annual Review of Plant Biology,2008,59:651-681
[3] Deinlein U,Stephan A B,Horie T,et al. Plant salt-tolerance mechanisms[J]. Trends in Plant Science,2014,19(6):371-379
[4] Hasegawa P M. Sodium (Na⁺) homeostasis and salt tolerance of plants[J]. Environmental & Experimental Botany,2013,92(8):19-31
[5] Cao B,Long D,Zhang M,et al. Molecular characterization and expression analysis of the mulberry Na⁺/H⁺ exchanger gene family[J]. Plant Physiology Biochem,2016,99:49-58
[6] Gupta B,Gupta B,Huang B. Mechanism of Salinity Tolerance in Plants:Physiological,Biochemical,and Molecular Characterization[J]. International Journal of Genomics,2014,2014(1):1-18
[7] Shi H,Quintero F J,Pardo J M,et al. The Putative Plasma Membrane Na⁺/H⁺ Antiporter SOS1 Controls Long-Distance Na⁺ Transport in Plants[J]. Plant Cell,2002,14(2):465-477
[8] 康浩,甘肖梅,潘文平,等.Na⁺/H⁺逆向转运蛋白与植物耐盐性的关系[J].湖南农业科学,2009,(2):11-14
[9] Zhu,J K. Plant salt tolerance[J]. Trends in Plant Science,2001,6:66-71
[10] Quintero F J,Pardo J M. Activation of the plasma membrane Na⁺/H⁺ antiporter Salt-Overly-Sensitive 1(SOS1) by phosphorylation of an auto-inhibitory C-terminal domain[J]. Proceedings of the National Academy of Sciences of the United States of America,2011,108(6):2611-2616
[11] Liu M,Wang T Z,Zhang W H. Sodium extrusion associated with enhanced expression of SOS1 underlies different salt tolerance between Medicago falcata and Medicago truncatula seedlings[J]. Environmental and Experimental Botany,2015,110:46-55
[12] Zhu J K. Regulation of ion homeostasis under salt stress[J]. Current Opinion in Plant Biology,2003,6(5):441-445
[13] Ji H,Pardo J M,Batelli G,et al. The Salt Overly Sensitive (SOS) pathway:established and emerging roles[J]. Molecular Plant,2013,6(2):275-286
[14] Nie W X,Xu L,Yu B J. A putative soybean GmsSOS1 confers enhanced salt tolerance to transgenic Arabidopsis sos1-1 mutant[J]. Protoplasma,2015,252(1):127-134
[15] Yue Y,Zhang M,Zhang J,et al.SOS1 gene overexpression increased salt tolerance in transgenic tobacco by maintaining a higher K⁺/Na⁺ ratio[J]. Journal of Plant Physiology,2012,169(3):255-261
[16] Shi H,Lee B H,Wu S J,et al. Overexpression of a plasma membrane Na⁺/H⁺ antiporter gene improves salt tolerance in Arabidopsis thaliana[J]. Nature Biotechnology,2003,21:81-85
[17] Oh D H,Leidi E,Zhang Q,et al. Loss of halophytism by interference with SOS1 expression[J]. Plant Physiology,2009,151:210-222
[18] Oh D H,Lee S Y,Bressan R A,et al. Intracellular consequences of SOS1deficiency during salt stress[J]. Journal of Experimental Botany,2010,61:1205-1213
[19] Shi H,Quintero F J,Pardo J M,et al. The putative plasma membrane Na⁺/H⁺ antiporter SOS1 controls long-distance Na⁺ transport in plants[J]. Plant Cell,2002,14:465-477
[20] Quintero F J,Martinez-Atienza J,Villalta I,et al. Activation of the plasma membrane Na⁺/H⁺ antiporter Salt-Overly-Sensitive 1(SOS1) by phosphorylation of an auto-inhibitory C-terminal domain[J]. Proceedings of the National Academy of Sciences,2011,108(6):2611-2616
[21] Shi H,Ishitani M,Kim C,et al. The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na⁺/H⁺ antiporter[J]. Proceedings of the National Academy of Sciences of the United States of America,2000,97(12):6896-6901
[22] Oh D H,Bohnert H J. Loss of halophytism by interference with SOS1 expression[J]. Plant Physiology,2009,151(1):210-222
[23] Kaur C,Kumar G,Kaur S,et al. Molecular cloning and characterization of salt overly sensitive gene promoter from Brassica juncea(BjSOS2)[J]. Molecular Biology Reports,2015,42(6):1139-1148
[24] Wu Y,Ding N,Zhao X,et al. Molecular characterization of PeSOS1:the putative Na⁺/H⁺,antiporter of Populus euphratica[J]. Plant Molecular Biology,2007,65:1-11
[25] 王生银.盐生植物盐地碱蓬质膜Na⁺/H⁺逆向转运蛋白基因的克隆及其表达模式分析[D].2012
[26] Zhou Y,Yin X,Duan R. SpAHA1 and SpSOS1 coordinate in transgenic yeast to improve salt tolerance[J]. Plos One,2015,10(9):1-14
[27] Ma Q,Li Y-X,Yuan H-J,et al. ZxSOS1 is essential for long-distance transport and spatial distribution of Na⁺and K⁺ in the xerophyte Zygophyllum xanthoxylum[J]. Plant Soil,2014,374:661-676
[28] Feki K,Quintero F J,Khoudi H,et al. A constitutively active form of a durum wheat Na⁺/H⁺ antiporter SOS1 confers high salt tolerance to transgenic Arabidopsis[J]. Plant Cell Reports,2014,33:277-288
[29] Atienza M J,Jiang X,Garciadeblas B,et al. Conservation of salt overly sensitive pathway in Rice[J]. Plant Physiol,2007,143:1001-1012
[30] Ratner A,Jacoby B. Effect of K⁺,its Counter Anion,and pH on Sodium Efflux from Barley Root Tips[J]. Jorunal of Experimental Botany,1976,27(5):843-852
[31] Wang H,Zhang G H,Wang X D,et al. Cloning and sequence analysis of plasma membrane Na⁺/H⁺ antiporter gene SOS1 from Aeluropus littoralis[J]. Frontiers of Agricultural Science and Engineering,2012,17(3):28-33
[32] Olías R,Zakia E,Jun L,et al. The plasma membrane Na⁺/H⁺ antiporter SOS1 is essential for salt tolerance in tomato and affects the partitioning of Na⁺ between plant organs[J]. Plant Cell Environment,2009,32:904-916
[33] Zhao X,Wei P,Liu Z,et al. Soybean Na⁺/H⁺,antiporter GmsSOS1,enhances antioxidant enzyme activity and reduces Na+,accumulation in Arabidopsis and yeast cells under salt stress[J]. Acta Physiologiae Plantarum,2017,39(1):3-11
[34] 黄坤勇,李杉杉,郭强,等.黄花草木樨MoSOS1基因克隆及表达分析[J].生物技术通报.2017,33(10):1-6
[35] Ma D M,Xu W R,Li H W,et al. Co-expression of the Arabidopsis SOS genes enhances salt tolerance in transgenic tall fescue (Festuca arundinacea Schreb.)[J]. Protoplasma,2014,251(1):219-231
[36] 马苏勇,祝建波,王爱英,等.大叶补血草质膜Na⁺/H⁺逆向转运蛋白基因SOS1的克隆及对番茄的遗传转化[J].生物技术通报,2010(9):111-115
[37] Yue Y,Zhang M,Zhang J,et al. SOS1gene overexpression increased salt tolerance in transgenic tobacco by maintaining a higher K⁺/Na⁺ ratio[J]. Journal of Plant Physiology,2012,169(3):255-261
[38] Shi H,Lee B H,Wu S J,et al. Overexpression of a plasma membrane Na⁺/H⁺ antiporter gene improves salt tolerance in Arabidopsis thaliana[J]. Nature Biotechnology,2003,1(1):81-85
[39] Xu H X,Jiang X Y,Zhan K H,et al. Functional characterization of a wheat plasma membrane Na⁺/H⁺ antiporter in yeast[J]. Archives of Biochemistry and Biophysics,2008,73(1):8-15
[40] Gao X,Ren Z,Zhao Y,et al. Overexpression of SOD2 increases salt tolerance of Arabidopsis[J]. Plant Physiology,2004,33(4):1873-1881
[41] Yadav N S,Shukla P S,Jha A,et al. The SbSOS1 gene from the extreme halophyte Salicornia brachiata enhances Na⁺ loading in xylem and confers salt tolerance in transgenic tobacco[J]. BMC Plant Biology,2012,12(1):188-206
[42] 孙文君,唐敏,任健,等.8份云南苜蓿属优异种质资源对铝胁迫的生理耐受响应研究[J].草地学报,2018,26(5):1190-1197
[43] 闫得朋,巩林,袁玉莹,等.不同时期喷施页面肥对紫花苜蓿生长、产草量和营养品质的影响[J].草地学报,2018,26(5):1255-1261
[44] 陈小芳,于德花,宁凯,等.盐胁迫下苜蓿种质资源萌发特性综合评价[J].草地学报,2017,25(5):1115-1125
[45] 苏颖,王丕武,董英山,等.苜蓿生物技术研究进展[J].分子植物育种,2004,2(5):733-739
[46] Livak K J,Schmittgen T D. Analysis of relative gene expression data using Real-Time Quantitative PCR and the 2^-△△CT method[J]. Methods,2001,25(4):402-408
[47] An J,Song A,Guan Z Y,et al. The over-expression of Chrysanthemum crissum CcSOS1 improves the salinity tolerance of chrysanthemum[J]. Molecular Biology Reports,2014,41(6):1-8
[48] Wu Y,Nan D,Xin Z,et al. Molecular characterization of PeSOS1:the putative Na⁺/H⁺ antiporter of Populus euphratica[J]. Plant Molecular Biology,2007,65(1-2):1-11
[49] Munns R,Sharp R E. Involvement of abscisic acid in controlling plant growth in soil of low water potential[J]. Plant Biology,1993,20:425-437
[50] Hu X L,Jiang M Y,Zhang J H,et al. Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H₂O₂ production in leaves of maize(Zea mays) plants[J]. New Phytologist,2007,173:27-38
[51] Guo K M,Olga Babourina,Zed Rengel. Na⁺/H⁺ antiporter activity of the SOS1 gene:lifetime imaging analysis and electrophysiological studies on Arabidopsis seedlings[J]. Physiologia Plantarum,2009,137(2):155-158
[52] Song A,Lu J,Jiang J,et al. Isolation and characterisation of Chrysanthemum crassum SOS1,encoding a putative plasma membrane Na⁺/H⁺ antiporter[J]. Plant Biology,2012,14:706-713

紫花苜蓿MsSOS1基因的克隆与表达分析

Cloning and Expression Analysis of MsSOS1 gene from Medicago sativa

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