耐盐促生菌筛选鉴定及对盐胁迫燕麦生长的影响

doi:10.11733/j.issn.1007-0435.2021.12.002

草地学报 ›› 2021, Vol. 29 ›› Issue (12): 2645-2652.DOI: 10.11733/j.issn.1007-0435.2021.12.002

耐盐促生菌筛选鉴定及对盐胁迫燕麦生长的影响

张银翠, 姚拓, 赵桂琴, 徐茜, 王振龙, 包康, 撖冬荣

甘肃农业大学草业学院, 甘肃兰州 730070

收稿日期:2021-05-06 修回日期:2021-07-15 出版日期:2021-12-15 发布日期:2022-01-06
作者简介:张银翠(1993-)，女，汉族，甘肃静宁人，博士，研究方向为草地生物多样性，E-mail：2840911465@qq.com
基金资助:
燕麦根部病害的生物防控技术研究（CARS-07-C-1）；甘肃省退耕还草科技支撑项目（GFG-2019-366-2）资助

Screening and Identification of Salt-tolerant Growth Promoting Rhizobacteria and its Effects on Oat Growth under Salt Stress

ZHANG Yin-cui, YAO Tuo, ZHAO Gui-qin, XU Xi, WANG Zhen-long, BAO Kang, HAN Dong-rong

College of Prataculture Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2021-05-06 Revised:2021-07-15 Online:2021-12-15 Published:2022-01-06
Contact: 姚拓,E-mail:yaotuo@gsau.edu.cn

摘要/Abstract

摘要： 为获得耐盐促生菌资源，探究对盐胁迫燕麦（Avena sativa L.）的促生作用。用选择性功能培养基复筛黑果枸杞（Lycium ruthenicum Murr.）根系的促生菌，16S rDNA分子生物学技术确定分类学地位。测定接种促生菌对燕麦根系形态、株高及地上生物量的影响。获得目标菌株2株，LrM1固氮酶活性117.0 nmol C₂H₄·h^-1·mL^-1，溶无机磷量40.0 mg·L^-1，分泌indole-3-acetic acid （IAA） 30.0 μg·mL^-1，无产1-aminocyclopropane-1-carboxylic acid（ACC）脱氨酶特性，耐盐性为9%，经鉴定为Paenibacillus peoriae；LrM2固氮酶活性280.0 nmol C₂H₄·h^-1·mL^-1，溶无机磷量5.6 mg·L^-1，具有产ACC脱氨酶的特性，耐盐性达11%，经鉴定为Bacillus属。不同盐浓度下各促生菌对燕麦的促生效果差异显著（P<0.05），LrM2在0.6% NaCl胁迫时显著促进总根长、根表面积、根体积和根尖数增加，株高和鲜重分别增加21.9%和24.7%（P<0.05）。LrM2有多种促生特性和高耐盐性，能够促进盐胁迫下燕麦的生长，具有开发成盐碱地微生物肥料的潜力。

关键词: 促生菌, 盐胁迫, 根系形态

Abstract: To obtain the resources of salt-tolerant plant growth-promoting rhizobacteria (PGPR),and to explore its growth-promoting effects on oats under salt stress. Using selective functional medium to screened PGPR which isolated from the roots of Lycium ruthenicum L.,and identified by 16S rDNA molecular biology. Effects of PGPR inoculation treatment on root morphology,shoot length and shoot fresh weight of oat were determined. Two target strains were screened，LrM1 was identified as Paenibacillus peoriae,solubility of inorganic phosphate was 40.0 mg·L^-1,production of IAA was 30.0 mg·L^-1 and the nitrogenase activity was 117.0 nmol C₂H₄·h^-1·mL^-1,didn’t produce 1-aminocyclopropane-1-carboxylic acid(ACC) deaminase. LrM2 was identified as Bacillus sp., solubility of inorganic phosphate was 5.6 mg·L^-1 and nitrogenase activity was 280.0 nmol C₂H₄·h^-1·mL^-1,was able to produce ACC deaminase. The growth-promoting effects of various bacteria on oat at different salt concentrations were significantly different (P<0.05). LrM2 significantly increased root length,root surface area,root volume,and root tips number under 0.6% NaCl stress,shoot length and shoot fresh weight increased by 21.9% and 24.7%,respectively (P< 0.05). The strain LrM2 has a variety of growth-promoting properties and high salt tolerance,which can promote the growth of oat under salt stress,and has the potential to be developed as microbial fertilizer in saline-alkali soil.

Key words: Growth promoting bacteria, Salt stress, Root morphology

中图分类号:

S543+.7

张银翠, 姚拓, 赵桂琴, 徐茜, 王振龙, 包康, 撖冬荣. 耐盐促生菌筛选鉴定及对盐胁迫燕麦生长的影响[J]. 草地学报, 2021, 29(12): 2645-2652.

ZHANG Yin-cui, YAO Tuo, ZHAO Gui-qin, XU Xi, WANG Zhen-long, BAO Kang, HAN Dong-rong. Screening and Identification of Salt-tolerant Growth Promoting Rhizobacteria and its Effects on Oat Growth under Salt Stress[J]. Acta Agrestia Sinica, 2021, 29(12): 2645-2652.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2021.12.002

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2021/V29/I12/2645

参考文献

[1] TAHIR M,AHMADA I,SHAHIDB M,et al. Regulation of antioxidant production,ion uptake and productivity in potato (Solanum tuberosum L.) plant inoculated with growth promoting salt tolerant Bacillus strains[J]. Ecotoxicology and Environmental Safety,2019(178):33-42
[2] YUAN QIN,IRINA S.DRUZHININA,XUEYU PAN,et al. Microbially mediated plant salt tolerance and microbiome based solutions for saline agriculture[J]. Biotechnology advanves,2016(34):1245-1259
[3] 李章雷,刘爽,王艳宇,等. 5株耐盐碱促生细菌的筛选鉴定及其对红小豆的促生作用[J].微生物学通报,2021,48(5):1580-1592
[4] 林丽,张裴斯,晋玲,等.黑果枸杞的研究进展[J].中国药房,2013,24(47):4493-4497
[5] 何芳兰,李治元,赵明,等.民勤绿洲盐碱化退耕地植被自然演替及土壤水分垂直变化研究[J].中国沙漠,2010,30(6):1374-1380
[6] KLOEPPER J W,SCHROTH M N,SAIKIA R. Relationship of in vitro antibiosis of plant growth-promoting rhizobacteria to plant growth and the displacement of root microflora[J]. Phytopathology,1981,71(10):1020-1024
[7] UPADHYAY S K,SINGH D P,SAIKIA R.et al. Genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition[J]. Current.microbiolgy.2009(59):489-496
[8] YASIN N A,AKRAM W,KHAN W U,et al. Halotolerant plant-growth promoting rhizobacteria modulate gene expression and osmolyte production to improve salinity tolerance and growth in Capsicum annum L[J]. Environmental Science and Pollution Research,2018,25(23):23236-23250
[9] SAPRE S,GONYIA-MISHRA I,TIWARI S. Klebsiella sp. confers enhanced tolerance to salinity and plant growth promotion in oat seedlings (Avena sativa)[J]. Microbiological Research,2018(206):25-32
[10] AFRIDI M S,MAHMOOD T,SALAM A,et al. Induction of tolerance to salinity in wheat genotypes by plant growth promoting endophytes:involvement of ACC deaminase[J].Plant Physiology and Biochemistry,2019(139):569-577
[11] KHAN M S,ASAF S,KHAN A L,et al. Alleviation of salt stress response in soybean plants with the endophytic bacterial isolate Curtobacterium sp. SAK1[J]. Annals of Microbiology,2019(69):797-808
[12] 刑国芳.植物根系发育及其激素调控机制[M].北京:中国农业科学技术出版社,2012:1-2
[13] MARCHNER H,KIRKBY E A,CAKMAK T. Effect of mineral nutritional status on shoot-root partitioning of photoassilates and cycling of mineral nutrients[J]. Journal of Experimental Botany,1996(47):1255-1263
[14] 毛齐正,杨喜田,苗蕾.植物根系构型的生态功能及其影响因素[J].河南科学,2008,26(2):172-176
[15] LYNCH J. Root architecture and plant productivity[J]. Plant Physiology,1995(109):7-13
[16] 廖红.菜豆磷效率的生理、形态构型和遗传特性[D].广州:华南农业大学,1998:62-76
[17] 张英,芦光新,谢永丽,等.溶磷菌分泌有机酸与溶磷能力相关性研究[J].草地学报,2015,23(5):1033-1038
[18] 马骢毓.民勤退耕区次生草地土壤微生物多样性研究及优势植物根际促生菌资源筛选[D].兰州:甘肃农业大学,2017:88
[19] PENROSE D M,GLICK B R. Methods for isolating and characterizing ACC deaminase containing plant growth promoting rhizobacteria[J]. Physiologia Plantarum,2003(118):10-15
[20] 王艳霞,解志红,张蕾,等.田菁根际促生菌的筛选及其促生耐盐效果[J].微生物学报,2020,60(5):1023-1035
[21] KOMARESOFLA B R,ALIKHANI H A,ETESAMI H,et al. Improved growth and salinity tolerance of the halophyte Salicornia sp. by co-inoculation with endophytic and rhizosphere bacteria[J]. Applied Soil Ecology,2019(138):160-170
[22] QIN S,FENG W W,ZHANG Y J,et al. Diversity of bacterial microbiota of coastal halophyte Limonium sinense and amelioration of salinity stress damage by symbiotic plant growth-promoting actinobacterium Glutamicibacter halophytocola KLBMP 5180[J]. Applied and Environmental Microbiology,2018,84(19):e01533-18
[23] 戢林,李廷轩,张锡洲,等.氮高效利用基因型水稻根系形态和活力特征[J].中国农业科学,2012,45(23):4770-4781
[24] GIEHL R F H,GRUBER B D,VON WIRÉN N. It's time to make changes:modulation of root system architecture by nutrient signals[J]. Journal of Experimental Botany,2014,65(3):769-778
[25] 杨小林,张希明,李义玲,等.塔克拉玛干沙漠腹地3种植物根系构型及其生境适应策略[J].植物生态学报,2008,32(6):1268-1276
[26] SZYMANSKA S,BORRUSO L,LORENZO B,et al. Bacterial microbiome of root-associated endophytes of Salicornia europaea in correspondence to different levels of salinity[J]. Environmental Science and Pollution Research,2018,25(25):25420-25431
[27] 郭正刚,张自和,王锁民,等.不同紫花苜蓿品种在黄土高原丘陵区适应性的研究[J].草业学报,2003,12(4):45-50
[28] MORAN J F,BECANA M,ITURBE-ORMAETXE I,et al. Drought induces oxidative stress in pea plants[J]. Planta,1994,194(3):346-352
[29] 张翠梅,师尚礼,刘珍,等.干旱胁迫对不同抗旱性苜蓿品种根系形态及解剖结构的影响.草地学报,2019,28(5):79-89
[30] ETESAMI H,BEATTIE G. Mining halophytes for plant growth-promoting halotolerant bacteria to enhance the salinity tolerance of non-halophytic crops[J]. Frontiers in microbiology,2018(9):1-20
[31] BAL H B,NAYAK L,DAS S,AAHYA T K. Isolation of ACC deaminase producing PGPR from rice rhizosphere and evaluating their plant growth promoting activity under salt stress[J]. Plant and Soil,2013(366):93-105
[32] GLICK B R. Bacteria with ACC deaminase can promote plant growth and help to feed the world[J]. Microbiological Research. 2014(169):30-39
[33] 李文娆,张岁岐,丁圣彦,等.干旱胁迫下紫花苜蓿根系形态变化及与水分利用的关系[J].生态学报,2010,30(19):5140-5150
[34] MU Z X,ZHANG S Q,HAO W F,et al. The effect of root morphological traits and spatial distribution on WUE in maize[J]. Acta Ecologica Sinica,2005,25(11):2895-2900

耐盐促生菌筛选鉴定及对盐胁迫燕麦生长的影响

Screening and Identification of Salt-tolerant Growth Promoting Rhizobacteria and its Effects on Oat Growth under Salt Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	赵树栋, 李建宏. 高原早熟禾根际促生菌分离筛选及特性研究[J]. 草地学报, 2021, 29(9): 1885-1891.
[2]	张新飞, 佘木子, 李晗玉, 敬松, 姜惠娜, 高浩, 朱延晓, 付娟娟. 琥珀酸黄杆菌促生机理及其对多年生黑麦草生长和抗逆性的生理调控作用[J]. 草地学报, 2021, 29(8): 1704-1711.
[3]	张万通, 李超群, 于露, 邵新庆. 植物根际促生菌菌肥在高寒草甸替代化肥效应研究[J]. 草地学报, 2021, 29(7): 1423-1429.
[4]	张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.
[5]	杨婉秋, 敬洁, 朱灵, 高永恒. 川西北高寒草甸植物根际促生菌筛选及其特性研究[J]. 草地学报, 2021, 29(6): 1174-1182.
[6]	刘允熙, 罗佳佳, 雷健, 刘国道, 刘攀道. 柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析[J]. 草地学报, 2021, 29(5): 876-883.
[7]	杨洁, 单立山, 苏铭, 张正中, 解婷婷, 李毅. 降水量和生长方式对红砂和珍珠根系形态特征的影响研究[J]. 草地学报, 2021, 29(5): 936-945.
[8]	潘平新, 倪强, 马瑞, 杨永义, 马彦军. 不同盐分处理对黑果枸杞种子萌发和幼苗生长的影响[J]. 草地学报, 2021, 29(2): 342-348.
[9]	万婷, 段钧译, 李蒙, 陈智勇. 南荻MlNAC2基因差异表达与耐盐生理响应的相关性分析[J]. 草地学报, 2021, 29(12): 2685-2693.
[10]	叶晓倩, 刘瑞曦, 冯金慧, 邓仕明, 李吉涛, 邓志军. 车前种子黏液对其劣变和萌发抗逆性的影响[J]. 草地学报, 2021, 29(12): 2728-2732.
[11]	王菲, 谭怡, 吕亚茹, 苏文欣, 姜宛彤, 刘宇乐, 严俊鑫. 水杨酸和赤霉素预处理对NaCl胁迫下神香草种子萌发的影响[J]. 草地学报, 2021, 29(12): 2862-2870.
[12]	余淑艳, 周燕飞, 黄薇, 王星, 高雪芹, 伏兵哲. 干旱、盐及旱盐复合胁迫对沙芦草光合和生理特性的影响[J]. 草地学报, 2021, 29(11): 2399-2406.
[13]	潘新雅, 李军保, 陈阳, 王鹏飞, 卫先伟, 李瑞, 刘佳茜, 郑智龙, 徐炳成, 王智. 6个紫花苜蓿品种根系形态结构对低磷胁迫的响应[J]. 草地学报, 2021, 29(11): 2494-2504.
[14]	朱依晗, 刘宁芳, 胡龙兴, 徐倩. 8份绿穗苋种子萌发期耐盐碱性综合评价[J]. 草地学报, 2021, 29(10): 2176-2183.
[15]	孙韵雅, 陈佳, 王悦, 程济南, 韩庆庆, 赵祺, 李惠茹, 李慧萍, 何傲蕾, 缑晶毅, 吴永娜, 牛舒琪, 索升州, 李静, 张金林. 根际促生菌促生机理及其增强植物抗逆性研究进展[J]. 草地学报, 2020, 28(5): 1203-1215.