南荻MlNAC2基因差异表达与耐盐生理响应的相关性分析

doi:10.11733/j.issn.1007-0435.2021.12.007

草地学报 ›› 2021, Vol. 29 ›› Issue (12): 2685-2693.DOI: 10.11733/j.issn.1007-0435.2021.12.007

南荻MlNAC2基因差异表达与耐盐生理响应的相关性分析

万婷¹, 段钧译^1,4, 李蒙^1,2,3, 陈智勇^1,2,3

1. 湖南农业大学, 湖南长沙 410128;
2. 芒属植物生态应用技术湖南省工程实验室, 湖南长沙 410128;
3. 国家能源非粮生物质原料研发中心湖南分中心, 湖南长沙 410128;
4. 湖南省蚕桑科学研究所, 湖南长沙 410127

收稿日期:2021-04-26 修回日期:2021-08-25 出版日期:2021-12-15 发布日期:2022-01-06
作者简介:万婷(1990-)，女，汉族，湖南长沙人，硕士研究生，主要从事芒属植物基因功能研究，E-mail:894072582@qq.com;段钧译(1993-)，女，汉族，湖南洞口人，硕士研究生，主要从事芒属植物遗传育种，E-mail:duanjunyi1222@163.com
基金资助:
国家自然科学基金面上项目（31471557）；国家自然科学基金青年项目（32000260）；湖湘高层次人才聚集工程（2019RS1051）；长沙市科技局国际合作重点项目（kq1901031）资助

Correlation Between MlNAC2 Gene Differential Expression and Salt-resistance Physiological Response in Miscanthus lutarioriparius Polyploid

WAN Ting¹, DUAN Jun-yi^1,4, LI Meng^1,2,3, CHEN Zhi-yong^1,2,3

1. Hunan Agricultural University, Changsha, Hunan Province 410128, China;
2. Miscanthus Ecological Application Technology, Hunan Engineering Laboratory, Changsha, Hunan Province 410128, China;
3. Hunan Branch, National Energy R&D Center for Non-food Biomass, Hunan Agricultural University, Changsha, Hunan Province 410128, China;
4. The Sericultural Research Institute of Hunan Province, Changsha, Hunan Province 410127, China

Received:2021-04-26 Revised:2021-08-25 Online:2021-12-15 Published:2022-01-06
Contact: 陈智勇,E-mail:zhiyongchen@hunau.edu.cn;李蒙,E-mail:mengli@hunau.edu.cn

摘要/Abstract

摘要： 为探究南荻（Miscanthus lutarioriparius）耐盐生理及其与基因表达的相关性，采用0%，0.2%，0.5%，0.8%浓度的NaCl处理奇岗和不同基因型南荻，测量NaCl溶液胁迫下的MlNAC2基因相对表达量和6个耐盐指标，并对二者之间的相关性进行分析。结果显示，南荻MlNAC2基因的表达量变化在不同材料之间差异较大，L2，L6，L9，P1出现10倍以上增加，而L1，L5，L8，L10变化量都在3倍以内。生理指标中叶绿素含量、净光合速率、气孔导度、脯氨酸与无胁迫对照相比均差异显著；丙二醛、超氧化物歧化酶在部分材料中与对照相比无显著差异。相关性分析表明，与MlNAC2基因的表达量变化最相关的生理指标是一系列光合作用相关因子：叶绿素含量、净光合速率、气孔导度。本研究结果可为探究南荻耐盐生理响应与分子机理层面的相互关系提供借鉴。

关键词: 盐胁迫, 南荻, 生理响应, MlNAC2基因, 相关性分析

Abstract: In this study,different Miscanthus lutarioriparius genotypes and Miscanthus×giganteus were used as test materials to explore the correlation between physiological responses and gene expression under salt stress. Those materials were treated with 0%,0.2%,0.5%,0.8% NaCl solutions individually. The expression of MlNAC2 gene and 6 physiological indexes were detected,and the correlation was analyzed. The results showed that the changes of MlNAC2 gene varied greatly among different materials,L2,L6,L9,and P1 increased more than 10 times while L1,L5,L8,and L10 changed less than 3 times. All materials were significantly different from the control in chlorophyll content,net photosynthetic rate,stomatal conductance,and proline,while some materials were not significantly different from the control in malondialdehyde content and superoxide dismutase activity. Correlation analysis showed that a series of photosynthetic factors referring to chlorophyll content,net photosynthetic rate,and stomatal conductance were related to MlNAC2 gene mostly. The results of this study can provide a reference for exploring the relationship between physiological response and molecular mechanism.

Key words: Salt stress, Miscanthus lutarioriparius, Physiological response, MlNAC2 gene, Correlation analysis

中图分类号:

Q78
Q945.78

万婷, 段钧译, 李蒙, 陈智勇. 南荻MlNAC2基因差异表达与耐盐生理响应的相关性分析[J]. 草地学报, 2021, 29(12): 2685-2693.

WAN Ting, DUAN Jun-yi, LI Meng, CHEN Zhi-yong. Correlation Between MlNAC2 Gene Differential Expression and Salt-resistance Physiological Response in Miscanthus lutarioriparius Polyploid[J]. Acta Agrestia Sinica, 2021, 29(12): 2685-2693.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2021.12.007

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2021/V29/I12/2685

参考文献

[1] YANG S,XUE S,KANG W W,et al. Genetic diversity and population structure of Miscanthus lutarioriparius,an endemic plant of China[J]. Plos One,2019,14(2):e211471
[2] ZUB H W,AMOULT S,BRANCOURT-HULMEL M. Key traits for biomass production identified in different Miscanthus species at two harvest dates[J]. Biomass and Bioenergy,2010,35(1):637-651
[3] SANG T. Toward the Domestication of Lignocellulosic Energy Crops:Learning from Food Crop Domestication[J]. Journal of Integrative Plant Biology,2011,53(2):96-104
[4] LONG X H,LIU L P,SHAO T Y,et al. Developing and sustainably utilize the coastal mudflat areas in China[J]. Science of The Total Environment,2016(6):569-570
[5] 张昆,李明娜,曹世豪,等.植物盐胁迫下应激调控分子机制研究进展[J].草地学报,2017,25(2):226-235
[6] 段俊枝,李莹,赵明忠,等. NAC转录因子在植物抗非生物胁迫基因工程中的应用进展[J].作物杂志,2017(2):14-22
[7] 方志红,刘建宁,张燕,等.白羊草NAC转录因子家族生物信息学分析[J].草地学报,2020,28(5):1266-1274
[8] 杨晓娜,田云,卢向阳. NAC转录因子在植物生长发育中的调控作用[J].化学与生物工程,2014,31(1):1-5,25
[9] TRAN L S,NISHIYAMA R,YAMAGUCHI-SHINOZAKI K,et al. Potential utilization of NAC transcription factors to enhance abiotic stress tolerance in plants by biotechnological approach[J]. GM Crops (Austin,TX),2010,1(1):32-39
[10] OLSEN A N,EMST H A,LEGGIO L L,et al. NAC transcription factors:structurally distinct,functionally diverse[J]. Trends in Plant Science,2005,10(2):79-87
[11] ZHENG X N,CHEN B,LU G J,et al. Overexpression of a NAC transcription factor enhances rice drought and salt tolerance[J]. Biochemical and Biophysical Research Communications,2009,379(4):985-989
[12] JI L,HU R,JIANG J,et al. Molecular cloning and expression analysis of 13 NAC transcription factors in Miscanthus lutarioriparius[J]. Plant Cell Reports,2014,33(12):2077-2092
[13] 陈静波,阎君,姜燕琴,等.暖季型草坪草优良选系和品种抗盐性的初步评价[J].草业学报,2009,18(5):107-114
[14] 陈超,赵丽丽,任登鸿,等.不同营养液对香根草生长的影响[J].贵州农业科学,2014,42(5):86-88
[15] 吉璐,陈智勇,肖亮,等. MlNAC2基因的克隆及其在南荻根部的表达模式[J].湖南农业大学学报(自然科学版),2016,42(1):27-32
[16] 严海东.柳枝稷内参基因筛选及NAC、HSP20基因家族挖掘与表达分析[D].成都:四川农业大学,2017:13-14
[17] 李得孝,员海燕,郭月霞,等.混合液浸提法测定玉米叶绿素含量的研究[J].玉米科学,2006,14(1):117-119
[18] 刘晓龙,徐晨,徐克章,等.盐胁迫对水稻叶片光合作用和叶绿素荧光特性的影响[J].作物杂志,2014(2):88-92
[19] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:7
[20] 张殿忠,汪沛洪,赵会贤.测定小麦叶片游离脯氨酸含量的方法[J].植物生理学通讯,1990(4):62-65
[21] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:164-169
[22] 段钧译,石艳兰,林宇环,等. NaCl胁迫下南荻多倍体的无机盐离子吸收特征[J].草业科学,2018,35(12):2893-2902
[23] 吉璐.南荻抗逆相关NAC转录因子的克隆及功能鉴定[D].长沙:湖南农业大学,2013:30-35
[24] 孙云飞,张文明,巢建国,等.盐胁迫对茅苍术叶绿素含量及叶绿素荧光参数的影响[J].江苏农业科学,2020,48(4):146-149
[25] 张景云,缪南生,白雅梅,等.盐胁迫下二倍体马铃薯叶绿素含量和抗氧化酶活性的变化[J].作物杂志,2014(5):59-63
[26] 沈徐悦,张浪,陈蓉蓉,等.盐胁迫对望春玉兰幼苗形态和相关生理指标的影响[J].浙江农林大学学报,2021,38(2):289-295
[27] VAN DER WEIJDE T,HUXLEY L M,HAWKINS S,et al. Impact of drought stress on growth and quality of miscanthus for biofuel production[J]. GCB Bioenergy.2017,9(4):770-782
[28] 宗俊勤,高艳芝,陈静波,等.芒属植物资源评价及盐胁迫对荻光合影响及其Na、K离子调控研究[C]//中国草学会能源草类专业委员会.能源草产业发展战略暨学术研讨会论文集.广西:中国草学会能源草类专业委员会,2014:101-103
[29] 宋倩云.冬青对盐胁迫的生理响应及耐盐筛选[D].南京:南京林业大学,2020:22
[30] KANAWAPEE N,SANITCHON J,LONTOM W,et al. Evaluation of salt tolerance at the seedling stage in rice genotypes by growth performance,ion accumulation,proline and chlorophyll content[J]. Plant and Soil,2012,358(1-2):225-239
[31] DE LACERDA C F,CAMBRAIA J,OLIVA M A,et al. Solute accumulation and distribution during shoot and leaf development in two sorghum genotypes under salt stress[J]. Environmental and Experimental Botany,2003,49(2):107-120
[32] 王丽媛,丁国华,黎莉.脯氨酸代谢的研究进展[J].哈尔滨师范大学自然科学学报,2010,26(2):84-89
[33] 朱金方,刘京涛,陆兆华,等.盐胁迫对中国柽柳幼苗生理特性的影响[J].生态学报,2015,35(15):5140-5146
[34] 李会云,郭修武.盐胁迫对葡萄砧木叶片保护酶活性和丙二醛含量的影响[J].果树学报,2008,25(2):240-243
[35] 彭立新,周黎君,冯涛,等.盐胁迫对沙枣幼苗抗氧化酶活性和膜脂过氧化的影响[J].天津农学院学报,2009,16(4):1-4
[36] HU H,DAI M,YAO J,et al. Overexpressing a NAM,ATAF,and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice[J]. Proceedings of the National Academy of Sciences of the United States of America,2006,103(35):12987-12992
[37] 卢敏.玉米ZmSNAC1和高粱SbSNAC1基因的克隆与功能分析[D].北京:中国农业科学院,2013:6-7
[38] 曾廷儒.玉米转高粱SbSNAC1基因抗旱性鉴定及其分子机制解析[D].北京:中国农业科学院,2020:6-7
[39] 毛佳昊,熊晓辉,卢一辰.茉莉酸调控植物应对逆境胁迫作用的研究进展[J].生物加工过程,2021,19(4):413-419,462

南荻MlNAC2基因差异表达与耐盐生理响应的相关性分析

Correlation Between MlNAC2 Gene Differential Expression and Salt-resistance Physiological Response in Miscanthus lutarioriparius Polyploid

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	姚世庭, 芦光新, 邓晔, 范月君, 周华坤, 党宁, 王英成, 张海娟, 颜珲璘. 模拟增温对高寒草地土壤原核生物群落组成及多样性影响[J]. 草地学报, 2021, 29(S1): 27-34.
[2]	张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.
[3]	李红, 李波, 方志坚, 杨曌, 张超, 冯成龙, 金姗姗, 王思琦, 石婉莹. 冻融和冻融+苏打盐碱复合胁迫下苜蓿幼苗的生理响应[J]. 草地学报, 2021, 29(7): 1469-1476.
[4]	潘平新, 倪强, 马瑞, 杨永义, 马彦军. 不同盐分处理对黑果枸杞种子萌发和幼苗生长的影响[J]. 草地学报, 2021, 29(2): 342-348.
[5]	张银翠, 姚拓, 赵桂琴, 徐茜, 王振龙, 包康, 撖冬荣. 耐盐促生菌筛选鉴定及对盐胁迫燕麦生长的影响[J]. 草地学报, 2021, 29(12): 2645-2652.
[6]	叶晓倩, 刘瑞曦, 冯金慧, 邓仕明, 李吉涛, 邓志军. 车前种子黏液对其劣变和萌发抗逆性的影响[J]. 草地学报, 2021, 29(12): 2728-2732.
[7]	王菲, 谭怡, 吕亚茹, 苏文欣, 姜宛彤, 刘宇乐, 严俊鑫. 水杨酸和赤霉素预处理对NaCl胁迫下神香草种子萌发的影响[J]. 草地学报, 2021, 29(12): 2862-2870.
[8]	余淑艳, 周燕飞, 黄薇, 王星, 高雪芹, 伏兵哲. 干旱、盐及旱盐复合胁迫对沙芦草光合和生理特性的影响[J]. 草地学报, 2021, 29(11): 2399-2406.
[9]	朱依晗, 刘宁芳, 胡龙兴, 徐倩. 8份绿穗苋种子萌发期耐盐碱性综合评价[J]. 草地学报, 2021, 29(10): 2176-2183.
[10]	卫昭君, 牛冰洁, 王永新, 赵祥, 朱慧森, 郭秀萍, 乔栋. 茉莉酸甲酯对盐胁迫下偏关苜蓿种子萌发和幼苗生长的影响[J]. 草地学报, 2020, 28(4): 998-1005.
[11]	贾会丽, 王学敏, 高涛, 石永红, 王运琦, 吴欣明, 刘建宁, 方志红, 董宽虎. 转MsLEA4-4基因拟南芥株系的耐盐性分析[J]. 草地学报, 2020, 28(3): 597-605.
[12]	李佩佩, 李毅, 苏世平, 种培芳, 李珍. 18个红砂家系的抗旱指标筛选与分析[J]. 草地学报, 2020, 28(2): 412-419.
[13]	卢锐, 李培英. 偃麦草盐胁迫下转录组分析[J]. 草地学报, 2020, 28(1): 31-44.
[14]	马彦军, 祝小娟, 何瑞雪. 盐爪爪种子萌发期抗盐性研究[J]. 草地学报, 2019, 27(5): 1237-1242.
[15]	李波, 方志坚, 李红, 杨瞾. ⁶⁰Co-γ辐射种子处理对‘龙牧806’苜蓿幼苗耐盐性的影响[J]. 草地学报, 2019, 27(5): 1259-1265.