草地早熟禾Phosphate Starvation Response 2基因的克隆及表达分析

doi:10.11733/j.issn.1007-0435.2022.01.004

草地学报 ›› 2022, Vol. 30 ›› Issue (1): 29-37.DOI: 10.11733/j.issn.1007-0435.2022.01.004

• 研究论文 • 上一篇

草地早熟禾Phosphate Starvation Response 2基因的克隆及表达分析

齐欣, 李春月, 刘本松, 刘博, 刘宇恒, 邸浩洋, 赵迪, 金忠民

齐齐哈尔大学生命科学与农林学院，黑龙江齐齐哈尔 161006

收稿日期:2021-07-15 修回日期:2021-08-20 发布日期:2022-01-27
通讯作者: 金忠民, E-mail: yyy6768@163.com
作者简介:齐欣(1997-)，女，汉族，黑龙江鹤岗人，硕士研究生，主要从事植物逆境生理及分子生物学研究，E-mail: qx15545625837@126.com
基金资助:
黑龙江省省属高等学校基本科研业务费科研项目(135509131)资助

Cloning and Expression Analysis of Phosphate Starvation Response 2 Gene in Poa pratensis L.

QI Xin, LI Chun-yue, LIU Ben-song, LIU Bo, LIU Yu-heng, DI Hao-yang, ZHAO Di, JIN Zhong-min

College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China

Received:2021-07-15 Revised:2021-08-20 Published:2022-01-27

摘要/Abstract

摘要： 磷饥饿响应(Phosphate starvation response PHR2)家族基因在植物磷(Pi)信号调节网络中发挥重要指示作用。为了解该基因在逆境胁迫中的反应机制，本研究从草地早熟禾(Poa pratensis L.)中克隆了PHR2基因，对其进行生物信息学和基因表达模式分析，并分析PHR2基因在草地早熟禾逆境胁迫中的作用。结果表明：草地早熟禾PHR2属于MYB-CC型转录因子，与节节麦氨基酸序列高度同源，该基因主要定位在细胞核。PHR2基因在草地早熟禾的根、茎、叶、穗中均有表达，其中根和穗中表达高于茎和叶；低磷诱导叶中PHR2基因表达低于适磷下该基因表达；干旱胁迫抑制该基因在根、叶的表达，但根与叶的转录调控模式存在差异。本研究为探究PHR家族基因在逆境胁迫中的功能提供一定的理论依据。

关键词: 草地早熟禾, 磷胁迫, 干旱胁迫, Phosphate Starvation Response 2, 转录因子

Abstract: Phosphorus starvation response family genes play important indication roles in plant phosphorus (Pi) signal regulation network. To understand the response mechanism of this gene under stress, PHR2 gene was cloned form Poa pratensis L.. Analyses of bioinformatics, expression profile, and functional role under stress of PHR2 gene were further carried out. Results showed that PHR2 gene in Poa pratensis L. belonged to the MYB-CC type transcription factor, its amino acid sequence was highly homologous to PHR2 in Aegilops tauschii Coss, and the PHR2 is mainly located in the nucleus. PHR2 gene was expressed in the roots, stems, leaves and panicles in Poa Pratensis, and the expression level in the roots and panicles was higher than that in the stems and leaves; the expression of PHR2 gene in leaves induced by low phosphorus was lower than that under suitable phosphorus; drought stress inhibited the expression of this gene in roots and leaves, which showing differences in transcriptional regulation patterns between roots and leaves. This study provides a theoretical basis for exploring the function of PHR family genes in abiotic stresses.

Key words: Poa pratensis L., Phosphorus stress, Drought stress, Phosphate Starvation Response 2, Transcription factor

中图分类号:

S344+.13

齐欣, 李春月, 刘本松, 刘博, 刘宇恒, 邸浩洋, 赵迪, 金忠民. 草地早熟禾Phosphate Starvation Response 2基因的克隆及表达分析[J]. 草地学报, 2022, 30(1): 29-37.

QI Xin, LI Chun-yue, LIU Ben-song, LIU Bo, LIU Yu-heng, DI Hao-yang, ZHAO Di, JIN Zhong-min. Cloning and Expression Analysis of Phosphate Starvation Response 2 Gene in Poa pratensis L.[J]. Acta Agrestia Sinica, 2022, 30(1): 29-37.

参考文献

[1] RUBIO V, LINHARES F, SOLANO R, et al. A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellularalgae[J]. Genes and Development, 2001, 15(16): 2122-2133
[2] CHENG L, BUCCIARELLI B, LIU J, et al. White lupin cluster root acclimation to phosphorus deficiency and root hair development involve unique glycerophosphodiester phosphodiesterases[J]. Plant Physiology, 2011, 156 (3): 1131-1148
[3] 刘允熙, 罗佳佳, 雷健, 等. 柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析[J]. 草地学报, 2021, 29(5): 876-883
[4] 吴欣明, 王运琦, 王赞, 等. 早熟禾种质资源形态多样性及主成分分析[J]. 中国农学通报, 2017, 33(3): 155-159
[5] 胡婧, 李德颖, 朱慧森, 等. 草地早熟禾叶和根茎浸提液对3种花卉植物种子萌发的影响[J]. 草地学报, 2019, 27(1): 178-184
[6] LIU M, SARTAIN J B, TRENHOLM L E, et al. Phosphorus Requirements of St. Augustinegrass Grown in Sandy Soils[J]. Crop Science, 2008, 48(3): 1178-1186
[7] THIRIET-RUPERT S, CARRIER G, CHENAIS B, et al. Transcription factors in microalgae: genome-wide prediction and comparative analysis[J]. BMC GenomicsVolume, 2016, 17(1): 282
[8] MA Q, XIA Z, CAI Z, et al. GmWRKY16 Enhances Drought and Salt Tolerance Through an ABA-Mediated Pathway in Arabidopsis thaliana[J]. Frontiers in Plant Science, 2019 (9): 1979-2025
[9] 吴年隆, 舒军, 王啸旗, 等. 谷子PHR家族基因的生物信息学及表达模式分析[J]. 山西农业科学, 2021, 49 (3): 257-264, 272
[10] CHIOU T J, LIN S I. Signaling network in sensing phosphate availability in plants[J]. Annual Review of Plant Biology, 2011, 62(1): 185-206
[11] CENTURY K, REUBER T L, RATCLIFFE O J. Regulating the Regulators: The Future Prospects for Transcription-Factor-Based Agricultural Biotechnology Products[J]. Plant Physiology, 2008, 147(1): 20-29
[12] BAI J, SUN F, WANG M, et al. Genome-wide analysis of the MYB-CC gene family of maize[J]. Genetica, 2019, 147(1): 1-9
[13] GUO M, RUAN W, LI C, et al. Integrative Comparison of the Role of the PHOSPHATE RESPONSE1 Subfamily in Phosphate Signaling and Homeostasis in Rice[J]. Plant Physiology, 2015, 168(4): 1762-1776
[14] XU Y, LIU F, HAN G, et al. Genome-wide identification and comparative analysis of phosphate starvation- responsive transcription factors in maize and three other gramineous plants[J]. Plant Cell Reports, 2018, 37 (5): 711-726
[15] WANG X H, BAI J R, LIU H M, et al. Overexpression of a Maize Transcription Factor ZmPHR1 Improves Shoot Inorganic Phosphate Content and Growth of Arabidopsis under Low-Phosphate Conditions[J]. Plant Molecular Biology Reporter, 2013, 31(3): 665-677
[16] LI S, WANG C, ZHOU L, et al. Oxygen deficit alleviates phosphate overaccumulation toxicity in OsPHR2 overexpression plants[J]. Journal of Plant Research, 2014, 127(3): 433-440
[17] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4): 402-408
[18] 李明明, 晋敏姗, 胡海斌, 等. 谷子MYB-CC基因家族的鉴定与表达分析[J]. 激光生物学报, 2021, 30(3): 259-269
[19] 王岩. PHR1-miR399-PHO2模块调控森林草莓(Fragaria vesca)磷含量的作用和机理[D]. 沈阳: 沈阳农业大学, 2019: 12-13
[20] NILSSON L, MULLER R, NIELSEN T H. Increased expression of the MYB-related transcription factor, PHR1, leads to enhanced phosphate uptake in Arabidopsis thaliana[J]. Plant, Cell & Environment, 2007, 30(12): 1499-512
[21] 周洁. 水稻低磷胁迫相关转录因子OsPHR1和OsPHR2的功能研究[D]. 杭州: 浙江大学, 2007: 13-18
[22] 焦芳婵. 水稻低磷胁迫相关转录因子OsPHR1和OsPHR2的研究[D]. 杭州: 浙江大学, 2005: 21-26
[23] 孙丽超. 拟南芥PHL2和PHR1共同调控对低磷胁迫的转录响应[D]. 北京: 清华大学, 2015: 10-12
[24] 王秀红. 玉米低磷应答转录因子ZmPHR1的克隆及功能分析[D]. 太原: 山西大学, 2013: 47-48
[25] 张宸辉, 周俊琴, 卢梦琪, 等. 油茶低磷响应转录因CoPHR1, CoPHR2基因的克隆与表达分析[J]. 植物生理学报, 2020, 56(4): 827-836
[26] HOLFORD I C R. Soil phosphorus: its measurement, and its uptake by plants[J]. Soil Research, 1997, 35(2): 227-240
[27] VANCE C P. Symbiotic nitrogen fixation and phosphorus acquisition. Plant nutrition in a world of declining renewable resources[J]. Plant Physiology, 2001, 127(2): 390-397
[28] 杨伟, 刘文辉, 马祥, 等. 干旱胁迫对2种不同抗旱性老芒麦幼苗ROS积累及抗氧化系统的影响[J]. 草地学报, 2020, 28(3): 684-693
[29] ZHOU J, JIAO F, WU Z, et al. OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants[J]. Plant Physiology, 2008, 146(4): 1673-1686
[30] SUN L, SONG L, ZHANG Y, et al. Arabidopsis PHL2 and PHR1 Act Redundantly as the Key Components of the Central Regulatory System Controlling Transcriptional Responses to Phosphate Starvation[J]. Plant Physiology 2016, 170(1): 499-514
[31] SEKHON R S, BRISKINE R, HIRSCH C N, et al. Maize gene atlas developed by RNA sequencing and comparative evaluation of transcriptomes based on RNA sequencing and microarrays[J]. Plos One, 2013, 8(4): e61005
[32] RUAN W, GUO M, WU P, et al. Phosphate starvation induced OsPHR4 mediates Pi-signaling and homeostasis in rice[J]. Plant Molecular Biology, 2017, 93(3): 327-340

草地早熟禾Phosphate Starvation Response 2基因的克隆及表达分析

Cloning and Expression Analysis of Phosphate Starvation Response 2 Gene in Poa pratensis L.

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	左郎, 王树森, 马迎梅, 温苏雅勒图, 白刚, 郁鑫明. 火炬树浸提液对两种草坪草种子萌发的影响[J]. 草地学报, 2021, 29(9): 1927-1933.
[2]	佟莉蓉, 倪顺刚, 任星远, 刘嘉欣, 闻静, 王娟, 宋雨. 褪黑素对干旱胁迫下达乌里胡枝子幼苗生长及叶片水分生理的影响[J]. 草地学报, 2021, 29(8): 1682-1688.
[3]	牟丹, 马戈亮, 卡着才让, 李宗仁, 忠克吉, 谢久祥, 李希来. 不同降温模式下高加索三叶草的转录组比较分析[J]. 草地学报, 2021, 29(7): 1386-1396.
[4]	金一锋, 陈阳, 齐欣, 高岩松, 金忠民, 赵清峰, 王琦. 草地早熟禾NRT1/PTR FAMILY 8.3基因的克隆及表达分析[J]. 草地学报, 2021, 29(7): 1397-1405.
[5]	朱琨, 郑桂华, 刘丽杰, 周璐, 李珊珊, 范震宇, 钟玥, 尼尼. 草地早熟禾叶片表皮解剖结构与抗白粉病性的研究[J]. 草地学报, 2021, 29(7): 1430-1435.
[6]	张韦钰, 王春勇, 杜红梅. 富氢水对草地早熟禾耐盐性的影响以及与抗氧化酶活性的关系[J]. 草地学报, 2021, 29(7): 1436-1445.
[7]	黄杰, 张郎织, 邢玉芬, 邹晓燕, 刘国道, 虞道耿, 陈志坚. 低磷胁迫对崖州硬皮豆生长及酸性磷酸酶活性的影响[J]. 草地学报, 2021, 29(7): 1462-1468.
[8]	朱琨, 刘丽杰, 周璐, 李珊珊, 范震宇, 吴纪一, 尼尼. 外源激素对染白粉菌草地早熟禾叶片膜透性及表面结构的影响[J]. 草地学报, 2021, 29(6): 1210-1216.
[9]	李鹏珍, 赵得琴, 邓波, 杨军, 赵国华, 赵亮. 生物炭与干旱胁迫对接种紫花苜蓿光合效率及生长的影响[J]. 草地学报, 2021, 29(6): 1257-1264.
[10]	刘允熙, 罗佳佳, 雷健, 刘国道, 刘攀道. 柱花草磷高效种质筛选及根系形态对低磷胁迫的响应分析[J]. 草地学报, 2021, 29(5): 876-883.
[11]	朱瑞婷, 牛奎举, 张娣君, 杨阳, 宋云, 马晖玲. 外源2,4-表油菜素内酯对镉胁迫下草地早熟禾叶绿素代谢的影响[J]. 草地学报, 2021, 29(4): 635-643.
[12]	黄睿, 罗佳佳, 吴远航, 刘攀道, 刘国道. 狼尾草属象草PpPAPs基因的克隆及响应低磷胁迫的表达模式分析[J]. 草地学报, 2021, 29(3): 425-433.
[13]	梁佳, 马依努·吾斯曼, 方志刚. 外源生长物质对干旱胁迫条件下甜高粱种子萌发的影响[J]. 草地学报, 2021, 29(3): 610-617.
[14]	叶晓倩, 刘瑞曦, 冯金慧, 邓仕明, 李吉涛, 邓志军. 车前种子黏液对其劣变和萌发抗逆性的影响[J]. 草地学报, 2021, 29(12): 2728-2732.
[15]	余淑艳, 周燕飞, 黄薇, 王星, 高雪芹, 伏兵哲. 干旱、盐及旱盐复合胁迫对沙芦草光合和生理特性的影响[J]. 草地学报, 2021, 29(11): 2399-2406.