木豆响应低温胁迫差异表达基因分析

doi:10.11733/j.issn.1007-0435.2022.07.011

草地学报 ›› 2022, Vol. 30 ›› Issue (7): 1701-1711.DOI: 10.11733/j.issn.1007-0435.2022.07.011

• 研究论文 • 上一篇

木豆响应低温胁迫差异表达基因分析

唐军^1,2, 丁西朋², 陈志坚², 马向丽¹, 毕玉芬¹, 郭凤根¹

1. 云南农业大学动物科学技术学院, 云南昆明 650201;
2. 中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室, 海南海口 571101

收稿日期:2022-01-19 修回日期:2022-04-13 发布日期:2022-08-02
通讯作者: 郭凤根,E-mail:yngfg@sina.cn
作者简介:唐军(1979-)，男，汉族，湖北洪湖人，博士研究生，副研究员，主要从事热带牧草及饲料作物育种研究，E-mail：tjtangjun@163.com
基金资助:
热带饲料作物与特色畜牧创新团队项目(NO.17CXTD-18)；非营利性科研机构改革专项启动费项目(No.0316051)；海南农业厅科技示范推广项目(牛羊饲草利用示范项目)；云南省重大科技专项绿色食品国际合作研究中心项目(2019ZG009)资助

Analysis of Differentially Expressed Genes in Pigeonpea [Cajanus cajan (L.) Millsp.] Responses to Cold Stress

TANG Jun^1,2, DING Xi-peng², CHEN Zhi-jian², MA Xiang-li¹, BI Yu-fen¹, GUO Feng-gen¹

1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;
2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Haikou, Hainan Province 571101, China

Received:2022-01-19 Revised:2022-04-13 Published:2022-08-02

摘要/Abstract

摘要： 为探讨木豆[Cajanus cajan (L.) Millsp.]响应低温胁迫的机制，本研究以1份耐低温(MD)和1份低温敏感(QZ)木豆为试验材料，经4℃低温胁迫处理后进行RNA-seq测序分析，结果表明：2份木豆RNA-seq获得了丰富的转录本信息，对转录因子和差异表达基因的基因功能进行注释。木豆响应响应低温胁迫的转录因子主要包括MYB，AP2-EREBP等；在两份材料中，GO分析发现DEGs注释在细胞组成中“膜”和生物学过程中“细胞过程”，KEGG分析均显著富集在“核糖体”通路中，而MD中还显著富集在植物激素信号转导、昼夜节律-植物等通路。通过两份材料对比，在MD材料中发现特有与耐低温相关的150个DEGs，主要为α-1，4-葡萄糖苷酶，参与碳水化合物、脂质代谢等途径。还筛选37个与耐寒相关重要差异表达基因和14条通路，并对10个DEGs进行qRT-PCR验证。本文从分子水平阐述了木豆低温胁迫下的响应机制，为未来木豆的抗寒育种奠定了理论基础。

关键词: 木豆, 低温胁迫, 转录组, 转录因子, 差异基因

Abstract: In this study,to explore the mechanisms of pigeonpea responses to cold stress,transcriptomic analysis was performed using two pigeonpea accessions subjected to a cold-stress treatment at 4℃. 'MD' is a cold tolerance accession and 'QZ' is a sensitive accession. The results showed that abundant information of transcripts were gotten and transcription factors and DEGs were annotated and identified also. The main transcription factors were MYB,AP2-EREBP etc. DEGs were significantly annotated in "membrane" of molecular function and in "cellular process" of biological process by GO,and significantly enriched in "ribosome" pathway in the two materials,and plant hormone signal transduction,circadian rhythm-plant and so on only in MD by KEGG. Through the comparison of the two materials,150 DEGs were found in the MD material,which were uiquely related to low temperature resistance,mainly alpha-1,4-glucosidase,which was involved in carbohydrate,lipid metabolism and other pathways. There were 37 candidate genes and 15 pathways related to cold tolerance in pigeonpea were also identified by comparison analysis,and 10 DEGs were verified by qRT-PCR. The paper expounds the molecular mechanisms of pigeonpea under low temperature stress at the molecular level,and lays a theoretical foundation for its cold-resistant breeding in the future.

Key words: Pigeonpea, Cold-stress, Transcriptome, Transcription factor, Differentially expressed genes

中图分类号:

S541

唐军, 丁西朋, 陈志坚, 马向丽, 毕玉芬, 郭凤根. 木豆响应低温胁迫差异表达基因分析[J]. 草地学报, 2022, 30(7): 1701-1711.

TANG Jun, DING Xi-peng, CHEN Zhi-jian, MA Xiang-li, BI Yu-fen, GUO Feng-gen. Analysis of Differentially Expressed Genes in Pigeonpea [Cajanus cajan (L.) Millsp.] Responses to Cold Stress[J]. Acta Agrestia Sinica, 2022, 30(7): 1701-1711.

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https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2022/V30/I7/1701

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木豆响应低温胁迫差异表达基因分析

Analysis of Differentially Expressed Genes in Pigeonpea [Cajanus cajan (L.) Millsp.] Responses to Cold Stress

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