不同磷水平对紫花苜蓿光合作用和根瘤固氮特性的影响

doi:10.11733/j.issn.1007-0435.2013.03.016

›› 2013, Vol. 21 ›› Issue (3): 512-516.DOI: 10.11733/j.issn.1007-0435.2013.03.016

不同磷水平对紫花苜蓿光合作用和根瘤固氮特性的影响

齐敏兴, 刘晓静, 张晓磊, 刘艳楠

甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070

收稿日期:2012-11-21 修回日期:2012-12-12 出版日期:2013-06-15 发布日期:2013-06-05
通讯作者: 刘晓静
作者简介:齐敏兴(1987- ),男,河北沧州人,硕士研究生,主要从事草坪与牧草营养生理研究,E-mail:qmx0602@126.com
基金资助:
甘肃省自然基金项目 "甘肃省秦王川灌区苜蓿草地施肥制度及其对环境的影响研究" (1010RJZA157);草业生态系统教育部重点实验室(甘肃农业大学)项目"供氮水平及氮素形态对紫花苜蓿养分吸收及根瘤特性的影响研究" (CYZS-2011012)资助

Effects of Different Phosphorus Levels on Photosynthesis and Root Nodule Nitrogen-fixing Characteristic of Alfalfa

QI Min-xing, LIU Xiao-jing, ZHANG Xiao-lei, LIU Yan-nan

College of Grassland Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Sino-U.S. Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2012-11-21 Revised:2012-12-12 Online:2013-06-15 Published:2013-06-05

摘要/Abstract

摘要： 采用砂培方法,在营养液条件下研究不同磷水平对甘农3号紫花苜蓿(Medicago sativa ‘Gannong No.3')光合作用和根瘤固氮特性的影响。结果表明:在其他营养充分供应的基础上,适当提高磷水平可以显著增大甘农3号紫花苜蓿叶片的气孔导度和光合速率,减小蒸腾速率,并提高叶片中叶绿素含量,增大叶面积,增加叶片数,同时使根瘤数目增多,根瘤重增加,固氮酶活性显著增强。光合作用在磷水平为1500 μmol·L^-1时最强,此时叶片蒸腾速率为4.27 mmol·m^-2·s^-1,气孔导度为347.54 mmol·m^-2·s^-1,光合速率为10.37 μmol·mol^-1,胞间CO₂浓度为307.28 μmol·mol^-1;叶绿素含量、叶面积和叶片数都随着磷水平的增大而呈现出先增大后减小的趋势,叶面积在磷水平为1500 μmol·L^-1时达到最大值1.21 cm²;叶片数在磷水平为250 μmol·L^-1时达到最大值26.50 个,继续增大磷浓度至1500 μmol·L^-1时叶片数仍显著高于对照;根瘤数和根瘤重随磷水平的增大持续增大;固氮酶活性在磷水平为1500 μmol·L^-1时最强达57.1 μg·g^-1·h^-1。综合考虑,磷水平在1500 μmol·L^-1时为最佳值,过高或过低的磷水平都不利于甘农3号紫花苜蓿的正常生长发育。

关键词: 磷水平, 紫花苜蓿, 光合作用, 固氮酶活性

Abstract: The effects of different phosphorus levels on photosynthesis and root nodule nitrogen-fixing characteristic of ‘Gannong No.3' alfalfa were investigated using nutrient culture. Results showed that stomatal conductance and photosynthetic rate were markedly increased with increased phosphorus levels, whereas transpiration rate was decreased. Simultaneously, chlorophyll content, leaf area, leaf number, root nodule number, root nodule weight and nitrogenase activity of alfalfa were improved. Photosynthesis was best at the phosphorus level of 1500 μmol·L^-1. Transpiration rate, stomatal conductance, photosynthetic rate and CO₂ concentration reached 4.27 mmol·m^-2·s^-1, 347.54 mmol·m^-2·s^-1,10.37 μmol·mol^-1 and 307.28 μmol·mol^-1 at this phosphate level. Chlorophyll content, leaf area and leaf number were first increased then decreased. Leaf area reached the largest (1.21 cm²) at 1500 μmol·L^-1 of phosphorous. Leaf number was 26.50 at the 250 μmol·L^-1, and increased with more phosphorus. Root nodule number and root nodule weight increased with the increase of phosphorus level. Nitrogenase activity was the best (57.1 μg·g^-1·h^-1) at 1500 μmol·L^-1. Phosphorus level of 1500 μmol·L^-1 is optimum for alfalfa. Excessive high and low phosphorus levels all prejudice normal growth and development of Gannong No.3 alfalfa.

Key words: Phosphorus levels, Alfalfa, Photosynthesis, Nitrogenase activity

中图分类号:

齐敏兴, 刘晓静, 张晓磊, 刘艳楠. 不同磷水平对紫花苜蓿光合作用和根瘤固氮特性的影响[J]. , 2013, 21(3): 512-516.

QI Min-xing, LIU Xiao-jing, ZHANG Xiao-lei, LIU Yan-nan. Effects of Different Phosphorus Levels on Photosynthesis and Root Nodule Nitrogen-fixing Characteristic of Alfalfa[J]. , 2013, 21(3): 512-516.

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参考文献

[1] 刘建中,杨振声,李继云.利用植物自身潜力提高土壤中磷的生物有效性[J].生态农业研究,1994,2(1):16-23
[2] 萧浪涛,王三根.植物生理学[M].北京:中国农业出版社,2004:71-162
[3] 王忠.植物生理学[M].北京:中国农业出版社,1999:88-135
[4] 李扬,孙洪仁,沈月,等.紫花苜蓿根系生物量垂直分布规律[J].草地学报,2012,20(5):793-799
[5] Sanginga N. Role of biological nitrogen fixation in legume based cropping systems: a case study of West Africa farming systems[J]. Plant and Soil,2003,252(3):25-39
[6] Darrell A M. Forage crops[M]. McGraw-Hill Book Company,1984:288-312
[7] 姜慧新,沈益新,翟桂玉,等.施磷对紫花苜蓿分枝生长及产草量的影响[J].草地学报,2009,17(5):588-592
[8] Pereira P A A, Bliss F A. Nitrogen fixation and plant growth of common bean (Phaseolus vulgaris L.) at different levels of phosphorus availability[J]. Plant and Soil,1987,104(1):79-84
[9] Hoagland D R, Arnon D I. The water culture method for growing plants without soil[J]. California Agricultural Experiment Station Bulletin,1938,347(1):1-39
[10] 王磊,白由路.不同氮处理春玉米叶片光谱反射率与叶片全氮和叶绿素含量的相关研究[J].中国农业科学,2005,38(11):2269-2270
[11] 陶洪斌,林杉.打孔称重法与复印称重法和长宽校正法测定水稻叶面积的方法比较[J].植物生理学通讯,2006,42(3):496-497
[12] 姚拓,张德罡,胡自治.高寒地区燕麦根际联合固氮菌研究Ⅰ:固氮菌分离及鉴定[J].草业学报,2004,13(2):106-111
[13] Foyer C H. Feedback inhibition of photosynthesis through source-sink regulation in leaves[J]. Plant Physiology and Biochemistry,1988,26(3):483-492
[14] Foyer C H. The basis for source-sink interaction in leaves[J]. Plant Physiology and Biochemistry,1987,25(5):649-657
[15] Rao I M, Arulanntham A R, Terry N. Leaf phosphate status. Photosznthesis and carbon partitioning in sugar beet Ⅱ. Diurnal changes in sugar phosphates, adenylates and nicotinamide nucleotides[J]. Plant Physiology,1989,90(3):820-826
[16] 王树起,韩晓增,乔云发,等.缺磷胁迫对大豆根瘤生长和结瘤固氮的影响[J].大豆科学,2009,28(6):1000-1003
[17] 温洋,金继运.施磷对紫花苜蓿光合特性以及生长的影响[J].中国土壤与肥料,2007(6):34-37
[18] Brooks A. Effects of phosphorus nutrition on ribulose-1,5-bisphosphate carboxylase activation, photosynthetic quanturn yield and amounts of some Calvin cycle metabolites in spinach leaves[J]. Plant Physiology,1986,13(2):221-237
[19] Foyer C H, Spencer C M. The relationship between phosphate status and photosynthesis in leaves[J]. Planta,1986,167(3):369-375
[20] Cockburn W, Baldry C W, Walker D A. Oxygen evolution by isolated chloroplasts with carbon dioxide as the hydrogen accepter. A requirement for orthophosphate or pyrophosphate[J]. Bichimica et Biophysica Acta,1967,131(3):584-596
[21] Cockburn W, Baldry C W, Walker D A. Some effects of inorganic phosphate on O₂ evolution by isolated chloroplasts[J]. Biochimica et Biophysica Acta-Bioenergetics,1967,143(3):614-624
[22] 刘丽君,孙聪姝.氮肥对大豆结瘤及叶片氮素积累的影响[J].东北农业大学学报,2005,36(2):133-137
[23] Tang C, Hinsinger P J, Drevon J J, et al. Phosphorus deficiency impairs early nodule functioning and enhances proton release in roots of Medicago truncatula L.[J]. Annals of Botany,2001,88(8):131-138
[24] 苗淑杰,乔云发,韩晓增.两种形态氮源条件下磷对大豆结瘤固氮的影响[J].大豆科学,2006,25(3):250-255

不同磷水平对紫花苜蓿光合作用和根瘤固氮特性的影响

Effects of Different Phosphorus Levels on Photosynthesis and Root Nodule Nitrogen-fixing Characteristic of Alfalfa

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