紫花苜蓿对牛角花齿蓟马为害的生理响应研究

doi:10.11733/j.issn.1007-0435.2015.01.023

草地学报 ›› 2015, Vol. 23 ›› Issue (1): 143-150.DOI: 10.11733/j.issn.1007-0435.2015.01.023

紫花苜蓿对牛角花齿蓟马为害的生理响应研究

寇江涛, 师尚礼

甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续研究中心, 甘肃兰州 730070

收稿日期:2014-03-14 修回日期:2014-06-04 出版日期:2015-02-15 发布日期:2015-01-31
通讯作者: 师尚礼
作者简介:寇江涛(1986-),男,甘肃镇原人,博士,研究方向为草种质资源及育种、牧草病虫害防治,E-mail:koujingtao@st.gsau.edn.cn
基金资助:
国家现代牧草产业体系建设专项(CARA-35)资助

Physiological Response of Medicago sativa to Odontothrips loti Damage

KOU Jiang-tao, SHI Shang-li

College of Grassland Science, Gansu Agricultral University, Key Laboratory of Ecosystem Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-Us Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China

Received:2014-03-14 Revised:2014-06-04 Online:2015-02-15 Published:2015-01-31

摘要/Abstract

摘要：

为明确紫花苜蓿(Medicago sativa)对牛角花齿蓟马(Odontothrips loti)为害的抗性生理机理,测定了不同牛角花齿蓟马虫口密度下抗蓟马苜蓿无性系R-1、感蓟马苜蓿无性系I-1可溶性蛋白、游离脯氨酸含量及超氧化物歧化酶(SOD)、愈创木酚过氧化物酶(GPX)、过氧化氢酶(CAT)活性的变化.结果表明:受牛角花齿蓟马为害后,R-1无性系在低虫口密度(1, 3头·枝条^-1)下,SOD, GPX活性升高,在高虫口密度(5, 7头·枝条^-1)下,SOD, GPX活性先上升后下降;I-1无性系各接虫处理的SOD, GPX活性均先上升后下降;二者各接虫处理的可溶性蛋白含量均降低,游离脯氨酸含量均增加,CAT活性均下降,且在牛角花齿蓟马为害过程中,R-1无性系各接虫处理可溶性蛋白的减少量、游离脯氨酸的增加量及CAT活性的下降速率均小于I-1无性系.说明SOD, GPX活性的升高是紫花苜蓿对牛角花齿蓟马诱导抗性的一种表现,R-1无性系在低虫口密度下其SOD, GPX活性持续升高而表现为抗蓟马,I-1无性系对蓟马为害的诱导抗性反应滞后于R-1无性系而表现为感蓟马.

关键词: 紫花苜蓿, 牛角花齿蓟马, 生理响应

Abstract:

In order to clarify the physiological resistance mechanism of alfalfa plant to thrip damage, the soluble protein contents, free proline contents as well as the SOD, GPX and CAT activities of resistant and susceptible alfalfa R-1 and I-1 clones growing under different thrip densities were investigated. The results indicated that the SOD and GPX activities of R-1 clones increased under low insect densities (1, 3 per branch), whereas the SOD and GPX activities of R-1 clones increased firstly and then decreased under high insect densities (5, 7 per branch); The SOD and GPX activities of I-1 treatments increased firstly and then decreased under all tested conditions. The soluble protein contents and CAT activities of R-1 and I-1 treatments decreased, whereas the free proline contents R-1 and I-1 treatments increased under all tested conditions. The CAT activities, soluble protein and free proline contents of R-1 clones changed less than that of I-1 clones under all tested conditions, indicating that the increases of SOD and GPX activities were the physiological response of alfalfa plant to thrip damage. R-1 clone under low thrip densities was resistant to thrip damage that manifested in the continuous increases of SOD and GPX activities, while I-1 clone was susceptible to thrip compared with R-1 clone.

Key words: Medicago sativa, Odontothrips loti, Physiological response

中图分类号:

S433

寇江涛, 师尚礼. 紫花苜蓿对牛角花齿蓟马为害的生理响应研究[J]. 草地学报, 2015, 23(1): 143-150.

KOU Jiang-tao, SHI Shang-li. Physiological Response of Medicago sativa to Odontothrips loti Damage[J]. Acta Agrestia Sinica, 2015, 23(1): 143-150.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2015.01.023

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2015/V23/I1/143

参考文献

[1] 洪绂曾. 苜蓿科学[M]. 北京:中国农业出版社,2009
[2] 吴永敷,赵秀华,特木尔布和. 蓟马是我国苜蓿生产的主要害虫[J]. 中国草地,1990(3):65-66
[3] 吴永敷,赵秀娴,赵秀华,等. 蓟马对苜蓿的危害[J]. 中国草地,1988(2):25-27
[4] 张蓉,马建华,王进华,等. 宁夏苜蓿病虫害发生现状及防治对策[J]. 草业科学,2003,20(6):40-44
[5] 严林,梅洁人. 青海省紫花苜蓿病虫种类及害虫天敌的调查[J]. 植物保护,1996,22(5):24-25
[6] 贺春贵,王森山,曹致中,等. 40个苜蓿品种(系)对蓟马田间抗性评[J]. 草业学报,2007,16(5):79-83
[7] 胡桂馨,贺春贵,王森山,等. 不同苜蓿品种对牛角花齿蓟马的抗性机制初步研究[J]. 草业科学,2007,24(9):86-89
[8] 胡桂馨,师尚礼,王森山,等. 不同苜蓿品种对牛角花齿蓟马的耐害性研究[J]. 草地学报,2009,17(4):505-509
[9] 寇江涛,胡桂馨,张新颖,等. 持续危害下抗、感蓟马苜蓿无性系大田生长特性的比较[J]. 草原与草坪,2011,31(4):35-40
[10] 寇江涛,师尚礼,胡桂馨,等. 抗、感蓟马苜蓿无性系对蓟马危害的补偿光合生理反应比较[J]. 昆虫学报,2011,54(8):910-917
[11] 陈建明,俞晓平,程家安,等. 植物耐虫性研究进展[J]. 昆虫学报,2005,48(2):262-272
[12] 陈青,张银东. 3种氧化酶与辣椒抗蚜性的相关性[J]. 热带作物学报,2004,25(3):42-46
[13] 贾贞,宋占午,金祖荫,等. 山楂叶螨危害对海棠叶片POD的影响[J]. 西北植物学报,2004,24(11):2136-2139
[14] 张金锋,薛庆中. 稻飞虱胁迫对水稻植株内主要保护酶活性的影响[J]. 中国农业科学,2004,37(10):1487-1491
[15] 吴青君,龚佑辉,徐宝云. 西花蓟马主要寄主植物可溶性糖和蛋白质含量测定[J]. 中国蔬菜,2007(10):20-22
[16] Bernays E A, Chapman R F. Host-plant selection by phytophagous insects [M]. London:United Kingdom,1994
[17] 刘裕强,江玲,孙立宏,等. 褐飞虱刺吸诱导的水稻一些防御性酶活性的变化[J]. 植物生理与分子生物学学报,2005,31(6):643-650
[18] 邢会琴,李敏权,徐秉良,等. 过氧化物酶和苯丙氨酸解氨酶与苜蓿白粉病抗性的关系[J]. 草地学报,2007,7(4):376-380
[19] 黄伟,贾志宽,韩清芳. 蚜虫(Aphis medicaginis Koch)危害胁迫对不同苜蓿品种体内丙二醛含量及防御性酶活性的影响[J]. 生态学报,2007,27(6):2177-2183
[20] 武德功,贺春贵,刘长仲,等. 不同苜蓿品种对豌豆蚜的生化抗性机制[J]. 草地学报,2011,19(3):497-501
[21] 武德功,王森山,刘长仲,等. 豌豆蚜刺吸胁迫对不同苜蓿品种体内单宁含量及生理活性的影响[J]. 草地学报,2011,19(2):351-355
[22] 邹琦. 植物生理学实验指导[M]. 北京:中国农业出版社,2006
[23] André Dias de Azevedo Neto, José Tarquinio Prisco, Joaquim Enéas-Filho, et al. Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants [J]. Journal of Plant Physiology,2005,162(10):1114-1122
[24] Giannopolitis C N, Ries S K. Superoxide dismutases. I. Occurrence in higher plants [J]. Plant Physiology,1977,59(2):309-314
[25] Urbanek H, Kuzniak-Gebarowska E, Herka K. Elicitation of defense responses in bean leaves by Botrytis cinerea polygalacturonase [J]. Acta Physiologiae plantarum,1991,13(1):43-50
[26] Beers J R F, Sizer I W. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase [J]. Journal of Biological Chemistry,1952,195(1):133-40
[27] 王娟,李德全. 逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报,2001,18(4):459-465
[28] 贺志理,王洪春. 盐胁迫下苜蓿中盐蛋白的诱导产生[J]. 植物生理学报,1991,17(1):71-79
[29] 汤章城. 逆境条件下植物脯氨酸的累积及其可能的意义[J]. 植物生理学通讯,1984,17(1):15-21
[30] Ashraf M, Mueen U D M, Warrich N. Production efficiency of mung bean (Vigna radiata L.) as affected by seed inoculation and NPK application [J]. International Journal Agriculture Biology,2003,5(2):179-180
[31] Cernusak L A, Aranda J, Marshall J D, et al. Large variation in whole plant water use efficiency among tropical tree species [J]. New Phytologist,2007,173(2):294-305
[32] Mc Neill S, Southwood T R E. Role of nitrogen in the development of insect-plant relationships [M]. Harbourne Biochemical Journal. Biochemical Aspects of Plant and Animal Coevolution. New York:Academic Press,1978:77-98
[33] William J, Mattson J. Herbivory in relation to plant nitrogen content [J]. Annual Review of Ecology and Systematics,1980,102(11):119-161
[34] 刘玉良,米福贵,特木尔布和,等. 苜蓿蓟马抗性与生理活性相关性研究[J]. 安徽农业科学,2009,37(18):8569-8571,8613
[35] 陈成升,谢志霞,刘小京. 旱盐互作对冬小麦幼苗生长及其抗逆生理特性的影响[J]. 应用生态学报,2009,20(4):811-816
[36] Wang X, Wang H, Wu F, et al. Effects of cinnamic acid on the physiological characteristics of cucumber seedlings under salt stress [J]. Front Agriculture China,2007,1(1):58-61
[37] 高必达,陈捷. 生理植物病理学[M]. 北京:科学出版社,2006
[38] Bowler C, Montagu M V, Inze D. Superoxide dismutase and stress tolerance [J]. Annual Review of Plant Physiology and Plant Molecular Biology,1992,43(1):83-116
[39] Bruce R J, West C A. Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragnents in suspension cultures caster bean [J]. Plant Physiology,1989,91(3):889-897
[40] Chen Z, Klessig D F. Identification of a soluble salicylic acidbinding protein thatmay function in signal transduction in the plant disease resistance response [J]. Proceedings of the National Academy of Science of USA,1991,88(18):8179-8183

紫花苜蓿对牛角花齿蓟马为害的生理响应研究

Physiological Response of Medicago sativa to Odontothrips loti Damage

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王静, 刘晓静, 程甜甜, 童长春, 汪雪. 不同氮效率紫花苜蓿叶特征及其产量效应研究[J]. 草地学报, 2021, 29(9): 1941-1949.
[2]	孟宣辰, 马鹏程, 马杰, 段珍, 韩阳阳, 张吉宇. 黄土高原半干旱区紫花苜蓿覆膜垄沟和施肥效应研究[J]. 草地学报, 2021, 29(9): 2098-2106.
[3]	陈雷, 暴雪艳, 郭刚, 霍文婕, 李清宏, 许庆方, 王聪, 刘强. 单宁酸和乳酸菌对紫花苜蓿青贮品质和体外瘤胃发酵的影响[J]. 草地学报, 2021, 29(8): 1853-1858.
[4]	李春萍, 邱轶辉, 夏厚胤, 谭璐娜, 许环宇, 张瀚文, 高景慧. 紫花苜蓿多父本F₁代低蒸腾及产量性状的隶属函数分析[J]. 草地学报, 2021, 29(7): 1416-1422.
[5]	李天琦, 林志玲, 卢轩, 黄佳媛, 杨宁, 沃野, 高凯, 柳小妮, 王显国. 灌溉量对科尔沁沙地紫花苜蓿土壤速效养分分布及淋失的影响[J]. 草地学报, 2021, 29(7): 1454-1461.
[6]	李红, 李波, 方志坚, 杨曌, 张超, 冯成龙, 金姗姗, 王思琦, 石婉莹. 冻融和冻融+苏打盐碱复合胁迫下苜蓿幼苗的生理响应[J]. 草地学报, 2021, 29(7): 1469-1476.
[7]	李鹏珍, 赵得琴, 邓波, 杨军, 赵国华, 赵亮. 生物炭与干旱胁迫对接种紫花苜蓿光合效率及生长的影响[J]. 草地学报, 2021, 29(6): 1257-1264.
[8]	王文静, 麻冬梅, 赵丽娟, 马巧利. 2,4-表油菜素内酯对盐胁迫下紫花苜蓿生理指标及根系离子积累的影响[J]. 草地学报, 2021, 29(6): 1363-1368.
[9]	潘新怡, 史昆, 龚攀, 韦宝, 吴欣明, 王赞. 紫花苜蓿花青苷合成的转录组分析[J]. 草地学报, 2021, 29(5): 866-875.
[10]	黄荣, 姚博, 张玉娟, 贾倩英, 李向阳, 张宏, 张振粉. 成团泛菌CQ10脂多糖对紫花苜蓿种子萌发及苗期生长的影响[J]. 草地学报, 2021, 29(5): 965-971.
[11]	徐洪雨, 甄莉丽, 李钰莹, 董宽虎, 李向林. 低温干旱环境对紫花苜蓿根颈耐寒性的影响[J]. 草地学报, 2021, 29(4): 724-733.
[12]	李满有, 马忠仁, 王斌, 杨雨琦, 沈笑天, 曹立娟, 李小云, 兰剑. 宁夏引黄灌区2个苜蓿品种不同行比混播模式研究[J]. 草地学报, 2021, 29(4): 798-804.
[13]	侯红雁, 申晨, 霍文婕, 刘强, 张拴林, 王聪, 陈雷, 许庆方, 王永新, 王忠豪, 郭刚. 同型发酵乳杆菌对低水分紫花苜蓿青贮发酵品质及体外消化率的影响[J]. 草地学报, 2021, 29(4): 835-841.
[14]	陈德奎, 郭香, 郑明扬, 陈晓阳, 周玮, 张庆. 砂仁精油对紫花苜蓿青贮品质的影响[J]. 草地学报, 2021, 29(4): 855-860.
[15]	夏方山, 王聪聪, 李红玉, 刘曼, 郑川, 张耀丹, 范华芳. 硒引发对紫花苜蓿种子抗氧化性能的影响[J]. 草地学报, 2021, 29(3): 472-477.