四倍体紫花苜蓿遗传图谱的初步构建

doi:10.11733/j.issn.1007-0435.2015.06.017

草地学报 ›› 2015, Vol. 23 ›› Issue (6): 1247-1251.DOI: 10.11733/j.issn.1007-0435.2015.06.017

四倍体紫花苜蓿遗传图谱的初步构建

王梦颖¹, 张铁军¹, 龙瑞才¹, 刘凤岐^1,2, 杨青川¹, 康俊梅¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 黑龙江省农业科学院草业研究所, 黑龙江哈尔滨 150086

收稿日期:2015-01-28 修回日期:2015-06-16 出版日期:2015-12-15 发布日期:2016-02-01
通讯作者: 康俊梅
作者简介:王梦颖(1988-),女,蒙古族,内蒙古丰镇市人,硕士研究生,研究方向为牧草资源与育种,E-mail:Wangmengying@163.com
基金资助:
国家牧草产业技术体系(CARS-35-04);国家自然科学基金(31402123);公益性行业(农业)科研专项(201403048)资助

Preliminary Construction of Genetic Map in Tetraploid Alfalfa

WANG Meng-ying¹, ZHANG Tie-jun¹, LONG Rui-Cai¹, LIU Feng-qi^1,2, YANG Qing-chuan¹, KANG Jun-mei¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Institute of Grassland Research, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China

Received:2015-01-28 Revised:2015-06-16 Online:2015-12-15 Published:2016-02-01

摘要/Abstract

摘要：

分别以早熟低产和晚熟高产苜蓿单株为父母本,通过人工杂交构建了四倍体紫花苜蓿(Medicago Sativa)F1遗传作图群体,采用单因子变量分析法,以降落式PCR和常规PCR结合的反应程序,建立了适宜于紫花苜蓿的分子标记扩增体系;应用130对SSR引物进行筛选,获得60对引物在父母本间存在多态性而被用于绘制遗传连锁图。采用PAGE电泳分析,对作图群体进行基因型分析。通过TetraploidMap软件对60个SSR标记进行连锁作图分析,有44个标记可以定位在8个连锁群上,占总标记数的33.8%,覆盖遗传距离979 cM,两标记间平均图距为22.25 cM,初步构建了四倍体紫花苜蓿遗传图谱的框架图,还需要进一步添加标记数量增大其饱和度,为重要性状的QTL定位奠定基础。

关键词: 紫花苜蓿, 遗传图谱, 简单重复序列(Simple Sequence Repeat, SSR), 基因分型

Abstract:

In this study, a F1 population of alfalfa was generated by crossing an early-maturing, low-yield plant (male parent) and a late-maturing, high-yielding plant (female parent). And a system of PCR amplification for tetraploid alfalfa was established by analyzing the standard PCR reaction program or touch-down program. Total of 130 SSR markers were tested in the experiments, of which 60 markers gave polymorphic between two parents and were used to construct the genetic linkage map. Genotypes of the F1 mapping population were analyzed by PAGE electrophoresis. And a prelimary genetic linkage map was constructed by using Tetraploid Map software. The genetic map consists of 44 loci, spanning 979 cM and covering all of 8 alfalfa chromosomes with an average genetic distance of 22.25 cM. More markers are still needed to be added to make a saturated genetic map. The study made a foundation for identifying important quantitative trait loci (QTL) for important agricultural traits.

Key words: Medicago sativa, Genetic mapping, Simple Sequence Repeat(SSR), Genotyping

中图分类号:

S541.9

王梦颖, 张铁军, 龙瑞才, 刘凤岐, 杨青川, 康俊梅. 四倍体紫花苜蓿遗传图谱的初步构建[J]. 草地学报, 2015, 23(6): 1247-1251.

WANG Meng-ying, ZHANG Tie-jun, LONG Rui-Cai, LIU Feng-qi, YANG Qing-chuan, KANG Jun-mei. Preliminary Construction of Genetic Map in Tetraploid Alfalfa[J]. Acta Agrestia Sinica, 2015, 23(6): 1247-1251.

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四倍体紫花苜蓿遗传图谱的初步构建

Preliminary Construction of Genetic Map in Tetraploid Alfalfa

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