青贮饲料中乳酸菌的分离鉴定及优良菌株筛选

doi:10.11733/j.issn.1007-0435.2011.01.024

›› 2011, Vol. 19 ›› Issue (1): 137-141.DOI: 10.11733/j.issn.1007-0435.2011.01.024

青贮饲料中乳酸菌的分离鉴定及优良菌株筛选

张慧杰¹, 玉柱², 王林¹, 蔡义民³, 李峰⁴, 陶雅⁴, 孙启忠⁴

1. 中国农业科学院研究生院, 北京, 100081;
2. 中国农业大学草地研究所, 北京, 100094;
3. 日本畜产草地研究所, 枥木县那须郡, 日本, 329-2793;
4. 中国农业科学院草原研究所, 内蒙古, 呼和浩特, 010010

收稿日期:2010-11-10 修回日期:2010-12-01 出版日期:2011-02-15 发布日期:2011-02-15
通讯作者: 孙启忠,E-mail:sunqz@126.com
作者简介:张慧杰(1986- ),女,内蒙古锡林郭勒盟人,硕士研究生,研究方向为牧草加工利用,E-mail:zhj439389793@yahoo.com.cn
基金资助:
现代农业产业技术体系建设专项资金;中央级公益性科研院所基本科研业务费专项资金;公益性行业(农业)科研专项经费项目(nyhyzx07-022);“948”项目(2010-G15);内蒙古自治区自然科学基金项目(2010MS0402)资助

Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains

ZHANG Hui-jie¹, YU Zhu², WANG Lin¹, CAI Yi-min³, LI Feng⁴, TAO Ya⁴, SUN Qi-zhong⁴

1. Graduate School, Chinese Academy of Agricultural Science, Beijing 100081, China;
2. China Agriculture University, Beijing 100094, China;
3. National Institute of Livestock and Grassland Science, Nasushiobara 329-2793, Japan;
4. Grassland Research Institute, CAAS, Hohhot, Inner Mongolia 010010, China

Received:2010-11-10 Revised:2010-12-01 Online:2011-02-15 Published:2011-02-15

摘要/Abstract

摘要： 为研究青贮饲料中乳酸菌的分类及形态学与生理生化特性,从苜蓿(Medicago sativa L.)及玉米(Zea maysL.)青贮饲料中分离得到5株乳酸菌。经16S rRNA序列分析,2株(GI7,GI24)为乳酸片球菌(Pediococcus acidilactici),其余3株(GI11,GI44,GI62)分别为戊糖片球菌(Pediococcus pentosaceus)、弯曲乳杆菌(Lactobacillus curvatus)和植物乳杆菌(Lactobacillus plantarum);5株菌均为同型发酵乳酸菌,除弯曲乳杆菌GI44在pH3.0,4.0,8.0等条件下不能生长外,其余菌株均可在5,10,40,45℃等不同温度条件下、pH3.0~8.0等环境下以及3.0%和6.5%NaCl溶液中良好生长或微弱生长。同时,通过产酸速率和生长曲线等指标筛选出产酸较快、生长迅速的GI62可作为制备乳酸菌青贮饲料添加剂的优良菌株。

关键词: 青贮, 乳酸菌, 优良菌株

Abstract: To study the taxonomic,morphologic,physiological and biochemical characteristics of lactic acid bacteria(LAB) in silage,five strains of LAB were isolated from alfalfa(Medicago sativa L.)and maize(Zea mays L.)silage.The 16S rRNA sequence analysis indicate that two strains are Pediococcus pentosaceus(GI7,GI24),and the other three are Pediococcus acidilactici,Lactobacillus curvatus and Lactobacillus plantarum(GI11,GI44,GI62).Each is homofermentative lactic acid bacteria and can grow well or weakly at 5,10,40,45℃,and with pH3.0~8.0,3.0% and 6.5% NaCl except that GI44 does not grow at pH3.0,4.0,8.0.Through measuring the rate of lactic acid production and the growth curve,GI62 was selected as eximious strain used for preparing lactic acid bacteria additive due to its rapid acid-production and growth speed.

Key words: Silage, Lactic acid bacteria, Eximious strain

中图分类号:

S816.53

张慧杰, 玉柱, 王林, 蔡义民, 李峰, 陶雅, 孙启忠. 青贮饲料中乳酸菌的分离鉴定及优良菌株筛选[J]. , 2011, 19(1): 137-141.

ZHANG Hui-jie, YU Zhu, WANG Lin, CAI Yi-min, LI Feng, TAO Ya, SUN Qi-zhong. Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains[J]. , 2011, 19(1): 137-141.

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参考文献

[1] 玉柱,贾玉山,张秀芬.牧草加工贮藏与利用[M].北京:化学工业出版社,2004.35-37
[2] Steen R W J,Unsworth E F,Gracey H L,et al.Evaluation studies in the development of a cammercial bacterial inoculant as an additive for grass silage.3.Response in growing cattle and interaction with protein supplementation[J].Grass and Forage Science,1989,44.381-390
[3] Yang X X,Chen H Z,Gao H L,et al.Bioconversion of corn straw by coupling ensiling and solid state fermentation[J].Bioresour Technology,2001,78:277-280
[4] Weinberg Z G,Ashbell G,Chen Y.Stabilization of returned dairy products by ensiling with straw and molasses for animal feeding[J].Journal of Dairy Science,2003,86:1325-1329
[5] Filya I.The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation,aerobic stability,and ruminal degradability of low dry matter corn and sorghum silages[J].Journal of Dairy Science,2003,86:3575-3581
[6] Cai Yimin.Identification and characterization of enterococcus species isolated from forage crops and their influence on silage fermentation[J].Journal of Dairy Science,1999,82:2466-2471
[7] Weinberg Z G,Szakacs G,Ashbell G,etal.The Effect of Lactobacillus buchneri and L.plantarum,applied at ensiling,on the ensiling fermentation and aerobic stability of wheat and sorghum silages[J].Journal of Industrial Microbiology & Biotechnology,1999,23:218-222
[8] 凌代文,东秀珠.乳酸细菌分类鉴定及实验方法[M].北京:中国轻工业出版社,1999
[9] Cai Y,Suyanandana P,Saman P,et al.Classification and characterization of lactic acid bacteria isolated from the intestines of common carp and fresh water prawns[J].Journal of General and Applied Microbiology,1999,45:177-184
[10] McDonald P,Henderson A R,Heron S J E.The biochemistry of silage[M].Abersytwyth,UK:Chalcombe Publications,1991
[11] Woolford M K.The detrimental effeets of air on silage[J].Journal of Applied Bacteriology,1990,68:101-112
[12] Anon.Silage additives supplement[J].Farmers Weekly,1988,54-57

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青贮饲料中乳酸菌的分离鉴定及优良菌株筛选

Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains

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