转苜蓿MsOr基因烟草光合-光响应和光合-CO2响应曲线特征研究

doi:10.11733/j.issn.1007-0435.2020.01.003

草地学报 ›› 2020, Vol. 28 ›› Issue (1): 20-30.DOI: 10.11733/j.issn.1007-0435.2020.01.003

转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究

赖帅彬¹, 潘新雅¹, 简春霞¹, 李军保², 郭尚洙³, 徐炳成^1,4, 王智^1,4

1. 西北农林科技大学水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西杨凌 712100;
2. 陕西省治沙研究所, 陕西榆林 719000;
3. 韩国生命工学研究院植物系统工程研究中心, 韩国大田 305-806;
4. 中国科学院水利部水土保持研究所, 陕西杨凌 712100

收稿日期:2019-10-18 修回日期:2019-11-02 出版日期:2020-02-15 发布日期:2020-01-18
通讯作者: 徐炳成, 王智
作者简介:赖帅彬(1996-),男,陕西富县人,硕士研究生,主要从事水土保持植被研究,E-mail:1147812232@qq.com
基金资助:
陕西省重点研发计划（2019TSLNY03-01）；国家自然科学基金（31700335）资助

Characteristics of Photosynthetic-Light Response and Photosynthetic-CO₂ Response Curves in Transgenic Alfalfa MsOr Gene Tobacco

LAI Shuai-bin¹, PAN Xin-ya¹, JIAN Chun-xia¹, LI Junbao², KWAK Sang-Soo³, XU Bing-cheng^1,4, WANG Zhi^1,4

1. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest Agriculture and Forestry University, Yangling, Shaanxi Province 712100, China;
2. Shaanxi Institute of Desertification Control, Yulin, Shaanxi Province 719000, China;
3. Plant Systems Engineering Research Center, Korea Research Institute of Biotechnology and Biotechnology, Daejeon 305-806, Korea;
4. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi Province 712100, China

Received:2019-10-18 Revised:2019-11-02 Online:2020-02-15 Published:2020-01-18

摘要/Abstract

摘要： 为明确苜蓿（Medicago sativa）Orange基因的表达对植物光合作用的影响，以转Orange（MsOr）基因型烟草（TOR1与TOR2）和野生型烟草（WT）为研究材料，采用盆栽控制试验，比较研究了WT，TOR1和TOR2叶片光响应曲线和CO₂响应曲线，并测定其叶片类胡萝卜素含量。结果表明：直角双曲线修正模型可较好地拟合烟草光响应曲线和CO₂响应曲线；在一定光合有效辐射强度和CO₂浓度范围内，随光强和CO₂浓度增加，TOR1和TOR2的净光合速率均显著高于WT（P<0.05）；TOR1和TOR2的最大净光合速率、光饱和点、CO₂饱和点、羧化效率、暗呼吸速率和光呼吸速率显著高于WT，而CO₂补偿点显著低于WT（P<0.05）；TOR1和TOR2的叶片总类胡萝卜素含量约为WT的2倍。研究表明：苜蓿MsOr基因的表达促进了烟草植株叶片类胡萝卜素的积累，同时提高了其光能和CO₂利用能力，增强了植株对高光强、高CO₂浓度环境的适应能力。

关键词: MsOr基因, 类胡萝卜素, 光合作用, 最大净光合速率, 光响应曲线, CO₂响应曲线

Abstract: In order to clarify the effect of alfalfa (Medicago sativa) Orange gene expression on plant photosynthesis,the leaf light response curves and CO₂ response curves of transgenic Orange (MsOr) (TOR1 and TOR2) and wild tobacco (WT) lines were analyzed through a pot experiment. The results showed that the right angle hyperbolic correction model could better fit the tobacco light response curve and CO₂ response curve than rectangular hyperbolic model and non-rectangular hyperbolic model. Over a range of photosynthetically active radiant and CO₂ concentration,the net photosynthetic rate of TOR1 and TOR2 were always significantly higher than that of WT (P<0.05) as the increase of light intensity and CO₂ concentration;maximum net photosynthetic rate,light saturation point,CO₂ saturation point,carboxylation efficiency,dark respiration rate,and photorespiration rate of TOR1 and TOR2 were significantly higher than those of WT,while the CO₂ compensation point was significantly lower than WT (P<0.05);meanwhile,the total leaf carotenoid content of TOR1 and TOR2 were about twice that of WT. These observations indicated that the expression of MsOr gene could efficiently improve tobacco plants adaptability through enhancing the leaf carotenoid content accumulation and the photosynthesis and CO₂ utilization ability under high light intensity and high CO₂ concentration condition.

Key words: MsOr gene, Carotenoids, Photosynthesis, Maximum net photosynthetic rate, Light response curve, CO₂response curve

中图分类号:

S311

赖帅彬, 潘新雅, 简春霞, 李军保, 郭尚洙, 徐炳成, 王智. 转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究[J]. 草地学报, 2020, 28(1): 20-30.

LAI Shuai-bin, PAN Xin-ya, JIAN Chun-xia, LI Junbao, KWAK Sang-Soo, XU Bing-cheng, WANG Zhi. Characteristics of Photosynthetic-Light Response and Photosynthetic-CO₂ Response Curves in Transgenic Alfalfa MsOr Gene Tobacco[J]. Acta Agrestia Sinica, 2020, 28(1): 20-30.

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转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究

Characteristics of Photosynthetic-Light Response and Photosynthetic-CO₂ Response Curves in Transgenic Alfalfa MsOr Gene Tobacco

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