偃麦草盐胁迫下转录组分析

doi:10.11733/j.issn.1007-0435.2020.01.004

草地学报 ›› 2020, Vol. 28 ›› Issue (1): 31-44.DOI: 10.11733/j.issn.1007-0435.2020.01.004

偃麦草盐胁迫下转录组分析

卢锐, 李培英

新疆农业大学, 草业与环境科学学院, 新疆草地资源与生态自治区重点实验室, 新疆乌鲁木齐, 830052

收稿日期:2019-09-02 修回日期:2019-10-14 出版日期:2020-02-15 发布日期:2020-01-18
通讯作者: 李培英
作者简介:卢锐(1991-),女,江苏淮阴人,硕士研究生,主要从事草坪草育种研究,E-mail:1356586119@qq.com
基金资助:
国家自然科学基金（31360580）资助

Transcriptome Analysis of Elytrigia under Salt Stress

LU Rui, LI Pei-ying

Xinjiang Agricultural University, College of Prataculture and Environmental Sciences, Xinjiang Key Laboratory of Grassland Resources and Ecology, Urumqi, Xinjiang 830052, China

Received:2019-09-02 Revised:2019-10-14 Online:2020-02-15 Published:2020-01-18

摘要/Abstract

摘要： 为探究偃麦草（Elytrigia repens）耐盐分子机制，本试验以耐盐差异显著的E36与E42为试验材料，对180 mmol·L^-1 NaCl处理0 d和3 d的叶片进行转录组测序。结果显示：转录组测序共获得80 735个Unigene，在NR数据库中共有10个同源性较高的物种；将差异表达基因进行KEGG功能注释，其中E42盐处理3 d和0 d对比产生的差异表达基因在植物病原体相互作用、淀粉和蔗糖代谢、苯丙烷类生物合成等方面有显著富集，E36盐处理3 d和0 d对比产生的差异表达基因在核糖体代谢、亚油酸代谢、碳代谢中具有显著富集；对E42盐处理0 d与3 d Unigenes进行对比，获得159个与耐盐相关差异表达基因，同样E36在盐处理下，获得61个与耐盐相关基因；对比代谢通路发现，E36和E42在光反应-捕光蛋白途径有明显差异；经qRT-PCR验证差异表达基因结果与转录组数据一致。偃麦草耐盐及敏盐种质在盐胁迫下光合调控、脯氨酸及过氧化物代谢在转录表达上均呈现显著差异。

关键词: 偃麦草, 转录组, 盐胁迫

Abstract: To explore the molecular mechanism of salt tolerance in Elytrigia repens,E36 and E42 with different salt tolerance were treated with 180 mmol·L^-1 NaCl for 0 h and 3 d. Transcriptome sequencing of Elytrigia repens leaves were performed to investigate the differences in transcriptional expression between the two materials under salt stress. The results showed that,transcriptome sequencing obtained a total of 80 735 Unigenes,and there were 10 homologous species in the NR database. The differentially expressed-genes (DEGs) were characterized by KEGG function. the DEGs of E42 salt between 3 d and 0 d were enriched in process of plant pathogen interaction,starch and Sucrose metabolism and phenylpropanoid biosynthesis;DEGs of E36 between 3d and 0d were enriched in ribosome metabolism,linoleic acid metabolism and carbon metabolism;159 DEGs related to salt tolerance were found between 0d and 3d salt treated E42,61 DEGs related to salt tolerance were obtained in E36 after treated with salt. There are significant differences in photoreaction and light-harvesting protein pathway between E36 and E42.,particularly LHCb1 (Light-harvesting complex II,LHCb) was differentially expressed. The results of several DEGs were verified by qRT-PCR and found to be consistent with transcriptome data. In summary,the expression of genes involved inphotosynthetic regulation,proline and peroxide metabolism of Elytrigia repens were altered by salt stress.

Key words: Elytrigia, Transcription group, Salt stress

中图分类号:

S543.9

卢锐, 李培英. 偃麦草盐胁迫下转录组分析[J]. 草地学报, 2020, 28(1): 31-44.

LU Rui, LI Pei-ying. Transcriptome Analysis of Elytrigia under Salt Stress[J]. Acta Agrestia Sinica, 2020, 28(1): 31-44.

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偃麦草盐胁迫下转录组分析

Transcriptome Analysis of Elytrigia under Salt Stress

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