PEG干旱胁迫对3种葱属植物种子萌发期渗透调节物质及酶活性的影响

doi:10.11733/j.issn.1007-0435.2021.01.009

草地学报 ›› 2021, Vol. 29 ›› Issue (1): 72-79.DOI: 10.11733/j.issn.1007-0435.2021.01.009

PEG干旱胁迫对3种葱属植物种子萌发期渗透调节物质及酶活性的影响

秦立刚¹, 李雪¹, 李韦瑶¹, 胡尧¹, 李俊², 崔国文¹

1. 东北农业大学动物科学技术学院, 黑龙江哈尔滨, 150030;
2. 中国农业科学院草原研究所, 内蒙古呼和浩特, 010000

收稿日期:2020-07-08 修回日期:2020-08-25 发布日期:2021-01-08
通讯作者: 崔国文
作者简介:秦立刚(1987-),男,山东蒙阴人,博士,副教授,主要从事草地植物资源管理与利用研究,E-mail:qinligang@neau.edu.cn
基金资助:
黑龙江省普通本科高等学校青年创新人才（UNPYSCT-2018144）资助

Effects of PEG Stress on Osmotic Regulation Substances and Enzyme Activities during Seed Germination of Three Allium L.

QIN Li-gang¹, LI Xue¹, LI Wei-yao¹, HU Yao¹, LI Jun², CUI Guo-wen¹

1. College of Animal Science and Technology, Northeast Agricultural University, Heilongjiang, Harbin Province 150030, China;
2. Grassland Research Institute of Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia 010000, China

Received:2020-07-08 Revised:2020-08-25 Published:2021-01-08

摘要/Abstract

摘要： 为探明东北地区常见野生葱属植物耐旱能力，筛选野生葱属植物耐旱种质资源，本研究以野韭（Allium ramosum L.）、山韭（Allium senescens L.）和蒙古韭（Allium mongolicum Regel）3种葱属植物为材料，采用不同浓度的PEG-6000模拟干旱胁迫，分析其种子萌发期渗透调节物质及酶活性的影响，并结合主成分分析和隶属函数分析比较抗旱性。结果表明：干旱胁迫下，3种葱属植物种子萌发期相对电导率显著升高（P<0.05），丙二醛、脯氨酸、可溶性糖、可溶性蛋白含量及超氧化物歧化酶、过氧化氢酶、过氧化物酶、多酚氧化酶活性均呈现先上升后下降的趋势。3种植物相比，山韭相对电导率最低，渗透调节物质积累量最高，酶活性变化明显。主成分分析表明，4种酶活性之间显示出强相关性。隶属函数分析得出3种植物种子的抗旱性由高到低依次为：山韭 > 野韭 > 蒙古韭。

关键词: 葱属植物, 干旱胁迫, 渗透调节物质, 酶活性

Abstract: In order to explore the drought tolerance of common wild species of Allium L. in Northeast China,and to find drought tolerant species of them,the seeds of Allium ramosum L., Allium senescens L. and Allium mongolicum Regel were selected as experimental materials. Different concentrations of PEG-6000 were used to simulate drought stress environment. The effects on osmotic adjustment substances and enzyme activities during seed germination were analyzed,and drought resistance was compared by principal component analysis and subordinate function analysis. The results showed that under drought stress,the relative electrical conductivity of three species of Allium L. increased significantly (P<0.05),the contents of malondialdehyde,proline,soluble sugar,soluble protein,the enzymatic activities of superoxide,catalase,peroxidase and polyphenol oxidase showed a trend of increasing first and then decreasing. Compared with the other two plants,the relative conductivity of Allium senescens L. was the lowest,the accumulation of osmoregulation substances were the highest,and the enzyme activities changed significantly. Principal component analysis showed that SOD,POD,CAT and PPO had a strong correlation. The order of drought resistance of the seeds from high to low was Allium senescens L. > Allium ramosum L. > Allium mongolicum Regel.

Key words: Allium species, Drought stress, Osmotic regulating substances, Enzyme activities

中图分类号:

秦立刚, 李雪, 李韦瑶, 胡尧, 李俊, 崔国文. PEG干旱胁迫对3种葱属植物种子萌发期渗透调节物质及酶活性的影响[J]. 草地学报, 2021, 29(1): 72-79.

QIN Li-gang, LI Xue, LI Wei-yao, HU Yao, LI Jun, CUI Guo-wen. Effects of PEG Stress on Osmotic Regulation Substances and Enzyme Activities during Seed Germination of Three Allium L.[J]. Acta Agrestia Sinica, 2021, 29(1): 72-79.

参考文献

[1] 于晶,温荣欣,闫庆鑫,等. 葱属植物活性物质及其生理功能研究进展[J]. 食品科学,2020,41(7):255-265
[2] 郝转. 葱属类植物研究进展[J]. 贵州农业科学,2017(11):110-113
[3] 黄修梅,郝丽珍,惠霖,等. 复合盐碱胁迫对蒙古高原野韭种子萌发的影响[J]. 北方园艺,2018(1):1-6
[4] 黄玉媛,杨绣娟,董琦,等. 采凉山山韭形态学鉴定及农艺性状和药食价值分析[J]. 黑龙江农业科学,2018(12):37-39
[5] 杨忠仁,刘建文,郝丽珍,等. 内蒙古地区四种葱属野菜花期物候及花营养成分研究[J]. 北方园艺,2013(8):1-4
[6] 黎祜琛,印治军. 树木抗旱性及抗旱造林技术研究综述[J]. 世界林业研究,2003,16(4):17-22
[7] 王亚楠,赵思明,曹兵. PEG-6000模拟干旱胁迫下10种草本植物萌发期抗旱性比较[J]. 草地学报,2020,28(4):983-989
[8] Pinto N C C,Campos L M,Evangelista A C S,et al. Antimicrobial Annona muricata L. (soursop) extract targets the cell membranes of Gram-positive and Gram-negative bacteria[J]. Industrial Crops & Products,2017,107:332-340
[9] 王海珍,徐雅丽,张翠丽,等. 干旱胁迫对胡杨和灰胡杨幼苗渗透调节物质及抗氧化酶活性的影响[J]. 干旱区资源与环境,2015,29(12):125-130
[10] Yan S P,Zhang Q Y,Tang Z C,et al. Comparative proteomic analysis provides new insights into chilling stress responses in rice[J]. Molecular & Cellular Proteomics,2006,5(3):484-496
[11] 杨伟,刘文辉,马祥,等. 干旱胁迫对2种不同抗旱性老芒麦幼苗ROS积累及抗氧化系统的影响[J]. 草地学报,2020,28(3):684-693
[12] Taskin T,Dogan M,Yilmaz B N,et al. Phytochemical screening and evaluation of antioxidant,enzyme inhibition,anti-proliferative and calcium oxalate anti-crystallization activities of Micromeria fruticosa spp. brachycalyx and Rhus coriaria[J]. Biocatalysis and Agricultural Biotechnology,2020,(27):101670
[13] Khodadadi F,Tohidfar M,Vahdati K,et al. Functional analysis of walnut polyphenol oxidase gene (JrPPO1) in transgenic tobacco plants and PPO induction in response to walnut bacterial blight[J]. Plant Pathology,2020,69(4):756-764
[14] 吴淼,刘信宝,丁立人,等. PEG模拟干旱胁迫下硅对紫花苜蓿萌发及生理特性的影响[J]. 草地学报,2017,25(6):1258-1264
[15] 刘爱荣,张远兵,谭志静,等. 模拟干旱对佛甲草生长和渗透调节物质积累的影响[J]. 草业学报,2012,21(3):156-162
[16] 刘新,符开欣,郭志慧,等. 6份川西北短芒披碱草种质苗期的抗旱性综合评价[J]. 草业科学,2017,34(1):101-111
[17] Ran C,Gulaqa A,Zhu J,et al. Benefits of biochar for improvingion contents,cell membrane permeability,leaf water status and yield of rice under saline-sodic paddy field condition[J]. Springer US,2020,39(1):370-377
[18] Płaz·ek A,Dubert F,Kopeć P,et al. Long-term effects of cold on growth,development and yield of narrow-leaf lupine may be alleviated by seed hydropriming or butenolide[J].Pubmed,2018,19(8):2416
[19] 任廷会,陈仕勇,罗兴雨,等. 外源NO对PEG胁迫下燕麦幼苗生长及生理特性的影响[J]. 种子,2020,39(5):30-34
[20] 王瑾,刘桂茹,杨学举. PEG胁迫下不同抗旱小麦品种幼苗形态及主要理化特性的比较[J]. 河北农业大学学报,2005,28(5):6-10
[21] Hassanpouraghdam M B,Mehrabani L V,Tzortzakis N. Foliar application of nano-zinc and iron affects physiological attributes of Rosmarinus officinalis and quietens NaCl salinity depression[J]. Journal of Soil Science and Plant Nutrition,2020,20(14):335-345
[22] 李州,彭燕,苏星源. 不同叶型白三叶抗氧化保护及渗透调节生理对干旱胁迫的响应[J]. 草业学报,2013,22(2):257-263
[23] Maach M,Baghour M,Akodad M,et al. Overexpression of LeNHX4 improved yield,fruit quality and salt tolerance in tomato plants (Solanum lycopersicum L.)[J]. Springer Netherlands,2020,47(4):4145-4153
[24] Silindile P Miya,Albert T Modi,Samson Z Tesfay,et al. Maize grain soluble sugar and protein contents in response to simulated hail damage[J]. Taylor & Francis,2018,35(5):377-383
[25] Grene A R,Neval E,Heath L S. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants[J]. Journal of Experimental Botany,2020,53(372):1331-1341
[26] Lichao C,Rong W,Jian F,et al. Transnitrosylation mediated by the non-canonical catalase ROG1 regulates nitric oxide signaling in plants[J]. Pubmed,2020,53(4):444-457
[27] Khan A A,Rahmani A H,Aldebasi Y H,et al. Biochemical and pathological studies on peroxidases-an updated review[J]. Global Journal of Health Science,2014,6(5):87-98
[28] 季杨,张新全,彭燕,等. 干旱胁迫对鸭茅根、叶保护酶活性、渗透物质含量及膜质过氧化作用的影响[J]. 草业学报,2014,23(3):144-151
[29] 陈爱萍,隋晓青,王玉祥,等. 干旱胁迫及复水对伊犁绢蒿幼苗生长及生理特性的影响[J]. 草地学报,2020,28(5):1216-1225
[30] Liu F,Han Q,Ni Y. Comparison of biochemical properties and thermal inactivation of membrane-bound polyphenol oxidase from three apple cultivars[J]. International Journal of Food Science & Technology,2017:1005-1012
[31] Zhang J,Zhang X,Ye M,et al. The jasmonic acid pathway positively regulates the polyphenol oxidase-based defense against tea geometrid caterpillars in the tea plant (Camellia sinensis)[J]. Journal of chemical ecology,2020,46(3):308-316

PEG干旱胁迫对3种葱属植物种子萌发期渗透调节物质及酶活性的影响

Effects of PEG Stress on Osmotic Regulation Substances and Enzyme Activities during Seed Germination of Three Allium L.

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