草地学报

草地学报 ›› 2022, Vol. 30 ›› Issue (9): 2298-2305.DOI: 10.11733/j.issn.1007-0435.2022.09.009

• 研究论文 • 上一篇    下一篇

外源H2O2引发对燕麦种胚线粒体AsA-GSH循环的影响

李红玉1,2, 王雅聪2,3, 夏方山2, 李尹琳2, 王聪聪2, 王勃2, 董宽虎2   

  1. 1. 山西农业大学科研管理部, 山西 太谷 030801;
    2. 山西农业大学草业学院, 山西 太谷 030801;
    3. 中国农业大学草业科学与技术学院, 北京 100193
  • 收稿日期:2022-01-17 修回日期:2022-04-04 出版日期:2022-09-15 发布日期:2022-09-30
  • 通讯作者: 夏方山,E-mail:dqlxfs8583@163.com
  • 作者简介:李红玉(1973-),女,山西晋中人,高级实验师,硕士,主要从事草类植物育种与种子科学研究,E-mail:lihongyu289@126.com
  • 基金资助:
    山西农业大学“十四五”生物育种工程项目（YZGC134）；国家自然基金项目（31702171）；山西省重点研发计划项目（201903D221091）；内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题（2021KF0303）资助

Influence of Exogenous H2O2 Priming on AsA-GSH Cycle in the Oat Embryonic Mitochondria

LI Hong-yu1,2, WANG Ya-cong2,3, XIA Fang-shan2, LI Yin-lin2, WANG Cong-cong2, WANG Bo2, DONG Kuan-hu2   

  1. 1. Office of Research Administration, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
    2. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
    3. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China
  • Received:2022-01-17 Revised:2022-04-04 Online:2022-09-15 Published:2022-09-30

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摘要： 本试验为了探究外源H2O2引发对燕麦（Avena sativa）种胚线粒体抗坏血酸-谷胱甘肽（Ascorbic acid-glutathione，AsA-GSH）循环的影响，设置浓度为0 mol·L-1，0.96 mol·L-1，1.92 mol·L-1，3.84 mol·L-1和7.68 mol·L-1 H2O2引发燕麦种子0 h （CK），6 h，12 h和18 h后，分析燕麦种胚线粒体AsA-GSH循环抗氧化酶活性及其脂质过氧化作用的变化规律。结果表明：外源H2O2引发导致了燕麦种胚线粒体H2O2及丙二醛含量的显著升高（P<0.05），其H2O2的清除则由其线粒体AsA-GSH循环来发挥主要作用，但其抗氧化能力的变化与外源H2O2引发的浓度和时间密切相关，低浓度（<0.96 mol·L-1）外源H2O2引发时间越短对燕麦种胚线粒体AsA-GSH循环抗氧化酶活性的促进效果就越小，而引发时间越长则其促进效果就越大；相反，高浓度（>3.84 mol·L-1）外源H2O2引发时间越短则对其抗氧化酶活性的抑制作用就越弱，而引发时间越长则其抑制作用就越强。因此，利用外源H2O2对燕麦种子进行引发时，H2O2浓度应低于1.92 mol·L-1、引发时间应少于12 h。

关键词: 燕麦, 过氧化氢, 种子引发, 线粒体, 抗坏血酸-谷胱甘肽循环

Abstract: To explore the effects of exogenous H2O2 priming on ascorbic acid-glutathione (AsA-GSH) cycle of seed embryonic mitochondria in oat (Avena sativa), seeds were primed with 0 mol·L-1, 0.96 mol·L-1, 1.92 mol·L-1, 3.84 mol·L-1 and 7.68 mol·L-1 H2O2 for 0 h (control), 6 h, 12 h and 18 h, then variation of antioxidant enzyme activities of the AsA-GSH cycle and lipid peroxidation in their embryonic mitochondria were measured. The results indicated that the H2O2 and malondialdehyde contents increased significantly in the embryonic mitochondria after exogenous H2O2 priming (P<0.05), and the scavenging of mitochondrial H2O2was mainly done by the AsA-GSH cycle, and the changes of its antioxidant capacity were closely related to the concentration and priming time of exogenous H2O2 priming. The shorter priming time at low concentration (<0.96 mol·L-1) of exogenous H2O2was, the less promotion effects on the antioxidant enzymes activities of AsA-GSH cycles in the embryonic mitochondria of oat seeds were. On the contrary, the shorter priming time at high concentration (>3.84 mol·L-1) of exogenous H2O2was, the weaker inhibition effects on the antioxidant enzyme activities of AsA-GSH cycle in the embryonic mitochondria of oat seeds were. Therefore, the concentration should be lower than 1.92 mol·L-1, and the priming time should be less than 12 h in the oat seed priming with exogenous H2O2.

Key words: Avena sativa, Hydrogen peroxide, Seed priming, Mitochondria, AsA-GSH cycle

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