草地学报 ›› 2022, Vol. 30 ›› Issue (12): 3233-3240.DOI: 10.11733/j.issn.1007-0435.2022.12.008

• 研究论文 • 上一篇    

藏沙蒿与多年生禾草混播对西藏沙化草地土壤酶活性和微生物生物量的影响

董凯1, 刘欢1, 刘云飞2, 王敬龙2, 仁增旺堆2, 王绮玉1, 罗建民1, 姚瑞瑞1, 张晓1   

  1. 1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室, 甘肃 兰州 730070;
    2. 西藏自治区农牧科学院草业科学研究所, 西藏 拉萨 850000
  • 收稿日期:2022-05-19 修回日期:2022-08-28 发布日期:2023-01-04
  • 通讯作者: 刘欢,E-mail:liuhuan@gsau.edu.cn;刘云飞,E-mail:306027640@qq.com
  • 作者简介:董凯(1998-),男,汉族,甘肃靖远人,硕士研究生,主要从事草地生物多样性研究,E-mail:344884621@qq.com
  • 基金资助:
    西藏自治区重点研发及转化计划-西藏藏沙蒿和大籽蒿资源评价与应用技术研究(XZ201902 NB01)资助

Effects of Mixed-Sowing of Artemisia wellbyi and Perennial Grasses on Soil Enzyme Activities and Microbial Biomass of Desertified Grassland in Tibet

DONG Kai1, LIU Huan1, LIU Yun-fei2, WANG Jing-long2, REN Zengwangdui2, WANG Qi-yu1, LUO Jian-min1, YAO Rui-rui1, ZHANG Xiao1   

  1. 1. College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou, Gansu Province 730070, China;
    2. Institute of Pratacultural Science, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa, Tibet 850000, China
  • Received:2022-05-19 Revised:2022-08-28 Published:2023-01-04

摘要: 为分析藏沙蒿(Artemisia wellbyi)与多年生禾草混播3年的土壤酶活性和微生物生物量的含量特征,筛选出修复效果最好的混播比例,本研究采用比色和氯仿熏蒸的方法分别测定不同处理下的土壤酶活性和微生物生物量,以藏沙蒿、垂穗披碱草(Elymus nutans)和细茎冰草(Agropyron trachycaulhum)不同比例混播为处理,分别为T1(4∶1∶2),T2(4∶2∶1)和T3(5∶1∶1),同时以未修复沙化草地为对照(CK),并与天然草地(NG)作比较。结果表明:与CK相比,3个比例混播处理均能改善土壤理化性质、提高土壤酶活性和微生物生物量碳氮磷含量,以T3处理的效果最好。相关性分析表明,土壤酶活性与有机质、速效钾和全氮呈正相关;土壤微生物生物量与有效磷、碱解氮和含水量呈正相关。因此,灌草混播时,适当增加灌木的占比有利于土壤养分及生物学特性的恢复。

关键词: 西藏, 生态修复, 酶活性, 土壤微生物量

Abstract: The characteristics of soil enzyme activity and microbial biomass content of desertified grassland mix-sown with Artemisia wellbyi and perennial grasses for 3 years were analyzed,and the mixing ratio with the best remediation effect was screened out. In this study,colorimetric and chloroform fumigation methods were used to determine soil enzyme activity and microbial biomass under different treatments. Artemisia wellbyi,Elymus nutans and Agropyron trachycaulhum were treated with different mixed sowing ratios,T1 (4:1:2),T2 (4:2:1),T3 (5:1:1). At the same time,unrepaired sandy grassland was used as control (CK) and compared with natural grassland (NG). The results showed that:Compared with CK,the three proportions of mixed sowing treatments could improve soil physical and chemical properties,increase soil enzyme activities and microbial biomass,carbon,nitrogen and phosphorus contents,and the effect of T3 treatment was the best. Correlation analysis showed that soil enzyme activities were positively correlated with organic matter,available potassium,and total nitrogen;soil microbial biomass was positively correlated with available phosphorus,alkalyzable nitrogen and water content. Therefore,properly increasing the proportion of shrubs in the mixture of shrub and grass is more conducive to the restoration of soil nutrients and biological characteristics.

Key words: Tibet, Ecological restoration, Enzyme activity, Soil microbial biomass

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