复合发酵剂对葵花副产物发酵品质及瘤胃降解率的影响

doi:10.11733/j.issn.1007-0435.2023.05.032

草地学报 ›› 2023, Vol. 31 ›› Issue (5): 1564-1570.DOI: 10.11733/j.issn.1007-0435.2023.05.032

复合发酵剂对葵花副产物发酵品质及瘤胃降解率的影响

李肖^1,2, 陈永成¹, 黄嵘峥¹, 齐亚银^1,3, 张凡凡¹, 马春晖¹

1. 石河子大学动物科技学院, 新疆石河子 832000;
2. 新疆伊犁州畜牧总站, 新疆伊宁 835000;
3. 新疆泰昆集团昌吉饲料有限责任公司, 新疆昌吉 831100

收稿日期:2022-10-26 修回日期:2022-12-12 出版日期:2023-05-15 发布日期:2023-05-31
通讯作者: 张凡凡,E-mail:zhangfanfan@shzu.edu.cn;马春晖,E-mail:chunhuima@126.com
作者简介:李肖(1998-)，男，汉族，新疆伊犁人，硕士研究生，主要从事饲草加工与生产研究，E-mail: 744364871@qq.com
基金资助:
农业农村部：国家现代农业产业技术体系项目(CARS)；新疆维吾尔自治区优质饲草产业技术体系项目(2022XJSC-Z-02)；新疆维吾尔自治区创新环境(人才、基地)建设专项(2021D14008)；昌吉州适度规模化肉牛育肥的技术集成与示范推广项目(2021Z02)资助

Effects of Compound Starter on Silage Quality and Rumen Degradation Rate of Sunflower Byproduct

LI Xiao^1,2, CHEN Yong-cheng¹, HUANG Rong-zheng¹, QI Ya-yin^1,3, ZHANG Fan-fan¹, MA Chun-hui¹

1. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China;
2. Xinjiang Yili Prefecture Animal Husbandry Station, Yining, Xinjiang 835000, China;
3. Xinjiang Taikun Group Changji Feed Co. Ltd, Changji, Xinjiang 831100, China

Received:2022-10-26 Revised:2022-12-12 Online:2023-05-15 Published:2023-05-31

摘要/Abstract

摘要： 研究添加复合发酵剂对葵花(Helianthus annuus)副产物微贮营养品质、发酵品质和瘤胃降解率的影响。以葵花副产物为试验材料,采用单因素试验设计,试验分为2个处理,即对照(CK处理)和添加复合发酵剂(SY处理),复合发酵剂为:植物乳杆菌(Lactobacillus plantarum)9.3×10⁵cfu·g^-1,糖蜜3.57%,尿素0.3%,纤维素酶0.14%。发酵60 d后开袋进行营养、发酵品质、微生物数量和瘤胃降解率分析。结果表明:(1)SY处理的可溶性碳水化合物、干物质、粗蛋白、乳酸、氨态氮含量及乳酸菌数量显著高于CK处理(P<0.05);中性洗涤纤维和酸性洗涤纤维含量、pH值及霉菌和酵母菌数量显著低于CK处理(P<0.05)。(2)SY处理在24和48 h的干物质降解率和有机物降解率显著高于CK处理(P<0.05)。在葵花副产物微贮中添加复合发酵剂,能够显著改善发酵品质,促进反刍家畜利用。

关键词: 葵花副产物, 复合发酵剂, 发酵品质, 瘤胃降解率

Abstract: The effects of compound starter on nutrient quality,fermentation quality and rumen degradation rate of micro-storage silage of sunflower byproducts were investigated. In the investigation,the experiment was divided into two treatments,namely the control (CK) and the addition of compound fermentation agent (SY). The compound fermentation agent was:Lactobacillus plantarum 9.3×10⁵cfu·g^-1,molasses 3.57%,urea 0.3%,cellulase 0.14%,respectively. After 60 days of fermentation,the fermentation bags were opened for the analysis of nutrition,fermentation quality,microbial quantity and rumen degradation rate. The results showed as follows:(1) the contents of the water soluble carbohydrate (WSC),dry matter (DM),crude protein (CP),lactic acid (LA) and NH₃-N and the number of lactic acid bacteria (LAB) of the silage under the SY treatment were significantly higher than those in CK treatment (P<0.05). The content of NDF and ADF,pH value and quantity of Mold and Yeast were significantly lower than those of CK treatment (P<0.05). (2) DMD and OMD of SY treatment at 24 and 48 h were significantly higher than those of CK treatment (P<0.05). It was concluded that the silage of sunflower byproducts under adding the compound starter could be significantly improved on its fermentation quality and be promoted its utilization by ruminants.

Key words: Sunflower byproducts, Compound starter, Fermentation quality, Rumen degradation rate

中图分类号:

S816.6

李肖, 陈永成, 黄嵘峥, 齐亚银, 张凡凡, 马春晖. 复合发酵剂对葵花副产物发酵品质及瘤胃降解率的影响[J]. 草地学报, 2023, 31(5): 1564-1570.

LI Xiao, CHEN Yong-cheng, HUANG Rong-zheng, QI Ya-yin, ZHANG Fan-fan, MA Chun-hui. Effects of Compound Starter on Silage Quality and Rumen Degradation Rate of Sunflower Byproduct[J]. Acta Agrestia Sinica, 2023, 31(5): 1564-1570.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2023.05.032

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2023/V31/I5/1564

参考文献

[1] RAFAEL H,MIYAGI E S,OLIVEIRA E,et al. Chemical changes in sunflower silage associated with different additives[J]. Acta Scientiarum Animal Sciences,2013,35(1):29-35
[2] 新疆维吾尔自治区统计局. 新疆统计年鉴2020[M]. 北京:中国统计出版社,2020:180-183
[3] LIANG Q,CUI J,LI H,et al. Florets of sunflower (Helianthus annuus L.):potential new sources of dietary fiber and phenolic acids[J]. Chemical Reviews,2013,61(4):3435-3442
[4] MUOZ-ALMAGRO N,PRODANOV M,WILDE P J,et al. Obtainment and characterisation of pectin from sunflower heads purified by membrane separation techniques[J]. Food Chemistry,2020(318):126476
[5] 许中畅. 向日葵花盘总黄酮的提取、纯化及其活性研究[D].长春:长春工业大学,2016:2-3
[6] 徐义通,何玉华,孟岩. 葵花盘作为饲料的加工处理方式及在不同种属动物中的应用[J]. 畜牧与饲料科学,2018,39(3):31-36
[7] 张佳,王园,安晓萍,等. 向日葵副产物的营养特性及在反刍动物中的应用[J]. 中国畜牧兽医,2021,48(3):916-924
[8] GANDRA J R,OLIVEIRA E R,GANGRA E R D S,et al. Inoculation of Lactobacillus buchneri alone or with Bacillus subtilis and total losses,aerobic stability,and microbiological quality of sunflower silage[J]. Journal of Applied Animal Research,2016,45(1):609-614
[9] 赵士萍,周敏,蒋林树. 青贮饲料添加剂的研究进展[J]. 中国农学通报,2016,32(20):6-10
[10] 刘婷婷,张振威,岳春旺,等. 添加糖蜜和青贮添加剂对"张杂谷"全株青贮发酵品质的影响[J]. 中国畜牧兽医,2017,44(5):1382-1387
[11] 叶杭,张文昌,陈国富,等. 绿汁发酵液和纤维素酶对香蕉茎十小麦麸混合青贮品质的影响[J]. 中国畜牧兽医,2014,41(10):85-90
[12] 唐振华,杨承剑,李孟伟,等. 植物乳杆菌、布氏乳杆菌对甘蔗尾青贮品质及有氧稳定性的影响[J]. 中国畜牧兽医,2018,45(7):1824-1832
[13] 谢展,穆麟,张志飞,等. 乳酸菌或有机酸盐与尿素复配添加对紫花苜蓿混合青贮的影响[J]. 草业学报,2021,30(5):165-173
[14] 李肖,陈永成,黄嵘峥,等. 向日葵副产物微贮复合发酵条件的响应曲面优化分析[J]. 动物营养学报,2022,34(7):4726-4736
[15] 卢德勋,谢崇文. 现代反刍动物营养研究方法和技术[M]. 北京:农业出版社,1991:69-76
[16] 刘建新,杨振海,叶均安,等. 青贮饲料的合理调制与质量评定标准[J].饲料工业,1999(03):4-7
[17] 中华人民共和国国家质量监督检验检疫总局. GB/T 14924.9-2001 实验动物配合饲料常规营养成分的测定[S]. 北京:中国标准出版社,2001:1-3
[18] BRODERICK G A,KANG J H. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media[J]. Journal of Dairy Science,1980,63(1):64-75
[19] 许庆方,周禾,玉柱,等. 贮藏期和添加绿汁发酵液对袋装苜蓿青贮的影响[J]. 草地学报,2006,14(2):129-133,146
[20] 陈天寿. 微生物培养基的制造与应用[M]. 北京:中国农业出版社,1995:179-184
[21] RIBEIRO S S,VASCONCELOS J T,MORAIS M G,et al. Effects of ruminal infusion of a slow-release polymer-coated urea or conventional urea on apparent nutrient digestibility situ degradability,and rumen parameters in cattle fed low-quality hay[J]. Animal Feed Science and Technology,2011,164(1/2):53-61
[22] 张适,吴琼,尤欢,等. 添加不同酶制剂对全株玉米青贮发酵品质的影响[J].中国畜牧兽医,2019,46(4):1045-1052
[23] 孙贵宾,常娟,尹清强,等. 纤维素酶和复合益生菌对全株玉米青贮品质的影响[J]. 动物营养学报,2018,30(11):4738-4745
[24] 李秀丽. 氢氧化钠/尿素预处理强化木质纤维素酶解的研究[D]. 广州:华南理工大学,2015:73-75
[25] MCDONALD P,HUNDERON A R,HERON S J E. The biochemistry of silage[M]. Marlow:Chalcombe Publications,1991:81-151,184-236
[26] ARBABI S,GHOORCH T. The effect of different levels of molas-ses as silage additives on fermentation quality of foxtail millet (Setaria italica) silage[J]. Asian Journal of Animal Science,2008,2(2):43-50
[27] BAYTOK E,AKSU T,KARSLI M A,et al. The effects of formic acid,molasses and inoculant as silage additives on corn silage composition and ruminal fermentation characteristics in sheep[J]. Turkish Journal of Veterinary and Animal Sciences,2005,29(2):469-474
[28] ZHANG F F,WANG X Z,LU W H,et al. Improved quality of corn silage when combining cellulose-decomposing bacteria and Lactobacillus buchneri during silage fermentation[J]. BioMed Research International,2019(17):4361358
[29] 张适,吴琼,尤欢,等. 添加混合酶制剂对全株玉米青贮发酵品质的影响[J]. 中国畜牧杂志,2020,56(6):119-122,129
[30] 荣辉,余成群,李志华,等. 添加糖蜜和尿素对象草青贮发酵品质的影响[J]. 草地学报,2012,20(5):940-946
[31] CATCHPOOLE V R,HENZELLL E F. Silage and silage-making from tropical herbage species[J]. Herbage Abstracts,1971,41(3):213-221
[32] KONCA Y,BEYZI S B,KALIBER M,et al. Chemical and nutritional changes in sunflower silage associated with molasses,lactic acid bacteria and enzyme supplementation[J]. Harran Tarım ve Gıda Bilimleri Dergisi,2015,19(4):223-231
[33] 郭海明,夏天婵,朱雯,等. 青贮添加剂对稻草青贮品质和有氧稳定性的影响[J]. 草业学报,2017,26(2):190-196
[34] YUNUS M,OHBA N,SHIMOJO M,et al. Effects of adding urea and molasses on napiergrass silage quality[J]. Asian-Australasian Journal of Animal Science,2000,13(11):1542-1547
[35] CESAREO S D,LANGTON S R. Kinetic properties of Helicobacter pylori urease compared with Jack bean urease[J]. FEMS Microbiology Letters,1992,78(1):15-21
[36] LI H H,GUO Y N,TONG Z. Study on the biological degradation effect of corn straw[J]. Journal of Polytechnic University,2010(3):294-297
[37] MA H,MIAO S J,LIU J. Effect of cellulase on nutrient content of corn straw silage and rumen degradability of dairy cows[J]. Grazing Livestock,2006,133(4):43-46
[38] 牟怡晓,林语梵,张桂杰. 不可消化中性洗涤纤维在反刍动物生产中的应用及研究进展[J]. 动物营养学报,2020,32(11):5069-5074
[39] 王挺,宋磊,王旭哲,等. 番茄皮渣与高粱秸秆混贮的发酵品质、瘤胃降解率及有氧[J]. 动物营养学报,2022,34(3):1809-1819

复合发酵剂对葵花副产物发酵品质及瘤胃降解率的影响

Effects of Compound Starter on Silage Quality and Rumen Degradation Rate of Sunflower Byproduct

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	柴继宽, 赵桂琴, 琚泽亮. 添加不同乳酸菌对燕麦低温青贮发酵的影响[J]. 草地学报, 2023, 31(3): 923-928.
[2]	孙蓉, 刘悦, 陈婷, 吕仁龙, 周汉林, 李茂, 字学娟. 乳酸菌和糖蜜对构树叶青贮品质和细菌多样性的影响[J]. 草地学报, 2023, 31(1): 280-286.
[3]	李海萍, 关皓, 贾志锋, 刘文辉, 陈仕勇, 徐美玲, 陈生翠, 严东海, 蒋永梅, 甘丽, 张珈敏, 周青平. 添加麦麸和乳酸菌对川西北高寒地区燕麦裹包青贮品质和有氧稳定性的影响[J]. 草地学报, 2023, 31(1): 302-310.
[4]	张佳伟, 贾玉山, 格根图, 王志军, 包健, 李宇宇, 彭冬, 王宇, 孙鹏波, 岳晓明. 不同丙酸浓度对天然牧草青贮品质及有氧稳定性的影响[J]. 草地学报, 2022, 30(9): 2522-2528.
[5]	龙仕和, 李雪枫, 潘俊歆, 常晔, 史孟丽, 黄乃馨, 崔智海, 王坚. 不同生长天数‘热研4号’王草青贮品质和微生物群落多样性研究[J]. 草地学报, 2022, 30(7): 1900-1908.
[6]	马淑敏, 焦婷, 师尚礼, 秦伟娜, 王正文, 赵生国, 祁娟. 混合型乳酸菌制剂对不同品种青饲玉米青贮品质的影响[J]. 草地学报, 2022, 30(6): 1558-1568.
[7]	贾宏定, 黄丽娟, 刘蕾, 孙镕基, 张志飞, 陈桂华. 不同品种水稻秸秆青贮品质的比较研究[J]. 草地学报, 2022, 30(5): 1310-1318.
[8]	赵牧其尔, 王志军, 包健, 孙林, 张永虎, 温蕊, 王伟, 贾玉山, 格根图. 不同青贮添加剂对饲用谷子青贮品质的影响[J]. 草地学报, 2022, 30(4): 1021-1026.
[9]	付东青, 贾春英, 张力, 张凡凡, 马春晖. 石河子地区青贮玉米农艺性状和发酵品质动态分析及评价[J]. 草地学报, 2022, 30(3): 682-691.
[10]	李彦飞, 杨双双, 初晓辉, 马向丽, 段新慧, 韩博, 段佳鑫, 单贵莲. 昆明优质本土乳酸菌的筛选及其对全株玉米青贮品质的影响[J]. 草地学报, 2022, 30(12): 3215-3224.
[11]	刘宇洋, 高文韩, 孔令芝, 郑杨晨, 刘畅, 何涛, 薛艳林, 李井春, 李雁冰. 化学添加剂对有氧胁迫下湿贮玉米发酵品质和有氧稳定性的影响[J]. 草地学报, 2022, 30(12): 3455-3463.
[12]	赵小雪, 王仕玉, 郭凤根, 罗富成. 乳酸菌及纤维素酶对不同藜麦秸秆青贮发酵品质的影响[J]. 草地学报, 2022, 30(10): 2827-2833.
[13]	吕竑建, 邹璇, 吴硕, 李云, 陈晓阳, 张庆. 添加辣木叶和香肠乳杆菌对玉米秸秆青贮品质的影响[J]. 草地学报, 2021, 29(6): 1343-1349.
[14]	王新宇, 申晨, 霍文婕, 刘强, 张拴林, 王聪, 陈雷, 许庆方, 王永新, 郭刚. 雁北地区不同品种玉米全株青贮发酵品质及细菌群落结构分析[J]. 草地学报, 2021, 29(5): 1087-1093.
[15]	尤思涵, 都帅, 周忠义, 格根图, 宛涛, 贾玉山. 高丹草与黑麦草适宜混贮比例研究[J]. 草地学报, 2021, 29(4): 821-827.