杏鲍菇菌糠和植物乳杆菌对全株玉米青贮有氧稳定性和体外发酵特性的影响

doi:10.11733/j.issn.1007-0435.2024.02.034

草地学报 ›› 2024, Vol. 32 ›› Issue (2): 646-653.DOI: 10.11733/j.issn.1007-0435.2024.02.034

杏鲍菇菌糠和植物乳杆菌对全株玉米青贮有氧稳定性和体外发酵特性的影响

武伟成¹, 肖定福¹, 陈一峰², 陈东¹, 温娟³, 黄纯勇⁴, 陈艳⁵, 徐志雄⁴, 魏仲珊⁶, 刘一然⁷

1. 湖南农业大学动物科学技术学院, 湖南长沙 410128;
2. 湖南省畜牧兽医研究所, 湖南长沙 410125;
3. 内蒙古林业和草原保护总站, 内蒙古呼和浩特 010000;
4. 湘西国家农业科技园区管理委员会, 湖南湘西土家族苗族自治州 416499;
5. 花垣县农业特色产业服务中心, 湖南湘西土家族苗族自治州 416499;
6. 湖南德人草业科技发展有限公司, 湖南常德 415000;
7. 湖南麻汝农产品销售有限公司, 湖南湘西土家族苗族自治州 416499

收稿日期:2023-08-24 修回日期:2023-11-29 出版日期:2024-02-15 发布日期:2024-03-06
通讯作者: 陈东,E-mail:chendong_326@126.com;肖定福,E-mail:xiaodingfu2001@163.com
作者简介:武伟成(1997-),男,汉族,河南郑州人,硕士研究生,主要从事反刍动物营养与饲料研究,E-mail:15737169546@163.com
基金资助:
中央引导地方科技发展专项资金项目（2023ZYC017，2021QZY037）；2021年湖湘英才（2021RC3139）；湖南省草食动物产业技术体系资助；湖南省重点研发计划项目（2022NK2025，2023NK2023）；山东省重点研发计划项目（2023TZXD046）；山东省中央引导地方科技发展专项资金项目（YDZX2022122）；国家肉牛牦牛产业技术体系（CARS-37）资助

Effects of the Addition of Pleurotus eryngii Mushroom Bran and Lactobacillus Plantarum on the Aerobic Stability and In Vitro Fermentation Characteristics of Whole Corn Silage

WU Wei-cheng¹, XIAO Ding-fu¹, ZHANG Zhi-fei², CHEN Dong¹, WEN Juan³, HUANG Chun-yong⁴, CHEN Yan⁵, XU Zhi-xiong⁴, WEI Zhong-shan⁶, LIU Yi-ran⁷

1. College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan Province 410128, China;
2. Hunan Institute of Animal Husbandry and Veterinary Medicine, Changsha, Hunan Province 410125, China;
3. Inner Mongolia General Forestry and Grassland Protection Station, Hohhot, Inner Mongolia 010000, China;
4. Management Committee of Xiangxi National Agricultural Science and Technology Park, Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province 416499, China;
5. Huayuan County Agricultural Specialty Industry Service Center, Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province 416499, China;
6. Hunan Dren Grass Technology Development Co., Ltd, Changde, Hunan Province 415000, China;
7. Hunan Ma Ru Agricultural Products Sales Co., Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province 416499, China

Received:2023-08-24 Revised:2023-11-29 Online:2024-02-15 Published:2024-03-06

摘要/Abstract

摘要： 试验旨在研究杏鲍菇菌糠和植物乳杆菌对全株玉米青贮有氧稳定性和体外发酵特性的影响。以玉米品种‘康农玉999’为青贮材料，添加杏鲍菇菌糠和植物乳杆菌混合青贮。青贮60 d后取样分析。对青贮前后发酵各组营养品质、发酵品质、有氧稳定性和体外消化效果进行研究。结果表明：青贮后，混合青贮发酵营养品质、乙酸含量和有氧稳定性时间优于全株玉米单独青贮；体外发酵试验中，添加10%杏鲍菇菌糠+植物乳杆菌0.02 g·kg^-1组CP降解率显著提高。由此可见，在全株玉米青贮中添加杏鲍菇菌糠和植物乳杆菌提高了青贮品质和有氧稳定性，改善了体外发酵特性，提高了CP降解率。综合考虑，以90%全株玉米+10%杏鲍菇菌糠+植物乳杆菌0.02 g·kg^-1混合青贮时可获得较好的效果。

关键词: 杏鲍菇菌糠, 植物乳杆菌, 全株玉米, 体外发酵, 有氧稳定性

Abstract: This experiment investigated the effects of pleurotus eryngii mushroom bran and plant lactobacillus on the aerobic stability and in vitro fermentation characteristics of whole-plant corn silage. The corn variety Kangnongyu 999 was used as silage material,and a mixture of mushroom bran and plant lactobacillus was added for silage. Sample were analyzed after 60 days of ensiling. The nutritional quality,fermentation quality,aerobic stability,and in vitro digestion effect of each fermentation group were studied before and after ensiling. The results showed that the fermentation nutritional quality,acetic acid content,and aerobic stability time of mixed silage were superior to those of the whole plant corn silage alone. In the in vitro fermentation experiment,the addition of 10% pleurotus eryngii mushroom bran and 0.02 g·kg^-1 plant lactobacillus significantly increased the CP degradation rate. Thus,adding pleurotus eryngii mushroom bran and plant lactobacillus to the entire corn silage improved the silage quality and aerobic stability,improved in vitro fermentation characteristics,and increased CP degradation rate. Taking all factors into consideration,a mixed silage of 90% whole plant corn,10% pleurotus eryngii mushroom bran,and 0.02 g·kg^-1 plant lactobacillus can achieve better results.

Key words: Pleurotus eryngii mushroom bran, Lactobacillus plantarum, Whole plant corn, In vitro fermentation, Aerobic stability

中图分类号:

S816.5+3

武伟成, 肖定福, 陈一峰, 陈东, 温娟, 黄纯勇, 陈艳, 徐志雄, 魏仲珊, 刘一然. 杏鲍菇菌糠和植物乳杆菌对全株玉米青贮有氧稳定性和体外发酵特性的影响[J]. 草地学报, 2024, 32(2): 646-653.

WU Wei-cheng, XIAO Ding-fu, ZHANG Zhi-fei, CHEN Dong, WEN Juan, HUANG Chun-yong, CHEN Yan, XU Zhi-xiong, WEI Zhong-shan, LIU Yi-ran. Effects of the Addition of Pleurotus eryngii Mushroom Bran and Lactobacillus Plantarum on the Aerobic Stability and In Vitro Fermentation Characteristics of Whole Corn Silage[J]. Acta Agrestia Sinica, 2024, 32(2): 646-653.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2024.02.034

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2024/V32/I2/646

参考文献

[1] 崔大为.青贮玉米的发展前景与种植技术[J].现代畜牧科技,2020,4:44-45
[2] VALVERDE M E,HERNANDEZ-PEREZ T,PAREDES-LOPEZ O.Edible mushrooms:improving human health and promoting quality life[J]. International Journal of Microbiology,2015:376-387
[3] 霍永红,蒋沙沙,卢卫红,等.食用菌污染因素分析及风险评估研究进展[J].食品安全质量检测学报,2022,13(10):3156-3163
[4] GAO X,TANG X,ZHU Q,et al. Biogas production from anaerobic co-digestion of spent mushroom substrate with different livestock manure[J]. Energies,2021,14(3):570-578
[5] 赵静,王延锋,孟祥海,等.我国食用菌工厂化生产现状与发展趋势[J].中国林副特产,2021(5):68-71,74
[6] LAM S S,LEE X Y,ROSLI M,et al. Microwave vacuum pyrolysis conversion of waste mushroom substrate into biochar for use as growth medium in mushroom cultivation[J]. Journal of Chemical Technology and Biotechnology,2019.94(5):1406-1415
[7] LEONG Y K,MA T W,YANG F C. Recent advances and future directions on the valorization of spent mushroom substrate (SMS):a review[J]. Bioresource Technology,2022,344:126-157
[8] 黄丽琴,李松桥,唐启源,等.全株水稻与平菇菌糠共发酵料对浏阳黑山羊屠宰性能、肉品质和器官指数的影响[J].草业学报,2021,30(6):133-140
[9] 钟港,陈东,田科雄,等.发酵菌糠替代白酒糟对育肥牛瘤胃发酵参数、微生物菌群结构和功能的影响[J].动物营养学报,2022,34(1):381-394
[10] HU W,SCHMIDT R J,MECIONELL E E,et al. The effect of Lactobacillus buchneri 4078 or Lactobacillus plantarum MTD-1 on the fermentation and aerobic stability of corn silages ensiled at two dry matter contents[J]. Journal Dairy Science,2009,92(8):3907-3914
[11] AOAC.AOAC official methods of analysis[M]. 15th ed.Arlington:Association of Official Analytical Chemists,1990:70-83
[12] VAN SOEST P J,ROBERTSON J B,LEWIS B A. Methods for dietary fiber,neutral detergent fiber,and non starch polysaccharides in relation to animal nutrition[J]. Journal Dairy Science,1991,74:3583-3597
[13] 董文成,林语梵,朱鸿福,等.不同品种葡萄渣对苜蓿青贮品质和有氧稳定性的影响[J].草业学报,2020,29(4):129-137
[14] MENKE K H,RAAB L,SALEWSLI A,et al. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro[J]. Journal of Agricultural Science,1979,93(1):217-222
[15] 邹诗雨,陈东,唐启源,等.青贮时间对再生稻头季全株青贮品质和体外瘤胃发酵特性的影响[J].动物营养学报,2020,32(07):3282-3290
[16] MUETZEL S,HUNT C L,TAVENDALE M H. Brief communication:evaluating rumen fluid from sheep and cattle as inoculum in a newly developed automated in vitro rumen batch culture system[J]. Proceeding of the New Zealand Society of Animal Production,2011,71:240-242
[17] MAKKAR H P S,SHARMA O P,DAWRA R K,et al. Simple determination of microbial protein in rumen liquor[J]. Journal Dairy Science,1982,65(11):2170-2173
[18] GUYADER J,BARON V S,BEAUCHEMIN K A. Corn forage yield and quality for silage in short growing season areas of the Canadian prairies[J]. Agronomy-basel,2018,8(9):164-189
[19] 郭江,孙颖琦,瓮巧云,等.青贮玉米和青贮谷子营养物质比较分析[J].饲料研究,2022,45(19):97-103
[20] 李成舰,蒋雪,杨大盛,等.混菌发酵对杏鲍菇菌糠营养成分及其体外瘤胃发酵的影响[J].湖南农业大学学报(自然科学版),2020,46(4):443-448
[21] TENNANT R K,SAMBLES C M,DIFFEY G E,et al. Metagenomic analysis of silage[J]. Jove-Journal of Visualized Experiments,2017,119:54936
[22] DRIEHUIS F,ELFER S J W H,SPOELSTRA S F. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with Lactobacillus buchneri inhibits yeast growth and improves aerobic stability[J]. Journal of Applied Microbiology,2010,87(4):583-594
[23] 王志敬,吴征敏,庄桂锋,等.不同精粗比日粮对雷州山羊体外瘤胃发酵的影响.中国畜牧兽医,2017,44(8):2303-2310
[24] HATEW B,CONE J W,PELLIKAAN W F,et al. Relationship between in vitro and in vivo methane production measured simultaneously with different dietary starch sources and starch levels in dairy cattle[J]. Animal Feed Science and Technology,2015,202:20-31
[25] LARSEN M L P,WEISBJERG M R,et al. Digestion site of starch from cereals and legumes in lactating dairy cows[J]. Animal Feed Science and Technology,2009,153:236-248
[26] 郑立鑫.日粮瘤胃可降解淀粉对奶山羊乳成分合成的影响及机制[D].杨凌:西北农林科技大学,2020:3
[27] 王铁.羊瘤胃胀气的诊断与治疗.农技服务,2017,34(23):124
[28] KIM M,MORRISON M,YU Z T. Status of the phylogenetic diversity census of ruminal microbiomes[J]. Fems Microbiology Ecology.2011,76(1):49-63
[29] LI F,WANG Z,WENG X,et al. Rumen bacteria communities and performances of fattening lambs with a lower or greater subacute ruminal acidosis risk[J]. Fronters in Microbiology,2017,8:2506-2516
[30] LI Y,HU X,CHA M,et al. Comparative analysis of the gut microbiota composition between captive and wild forest musk deer[J]. Fronters in Microbiology,2017,8:1705-1716
[31] CHEN L,DONG Z,SHAO T,et al. Ensiling characteristics,in vitro rumen fermentation,microbial communities and aerobic stability of low-dry matter silages produced with sweet sorghum and alfalfa mixtures[J]. Journal of the Science of Food and Agriculture,2019,99:2140-2151
[32] SUN P,LI J N,DU H,et al. Effects of bacillus subtilis natto and different components in culture on rumen fermentation and rumen functional bacteria in vitro[J]. Current Microbiology,2016,72:589-595
[33] SUN P,WANG J Q,DENG L F. Effects of bacillus subtilis natto on milk production,rumen fermentation,and tumimal microbiome of dairy cows[J]. Animal,2012,7:216-222
[34] XIE Y Y,WU Z Z,LIU J X,et al. Nitrogen partitioning and microbial protein synthesis in lactating dairy cows with different phenotypic residual feed intake[J]. Journal of Animal Science and Biotechnology,2019,10:356-364
[35] WANG C,HUANG Y X,YUE W B,et al. A study on the regulation of 2-methylbutyrate on the rumen fermentation in simmental steer[J]. Acta Laser Biology Sinica,2007,16(6):707-712
[36] KJAK K,PINO F,HEINRICHS A J. Effect of forage to concentrate ratio with sorghum silage as a source of forage on rumen fermentation,N balance,and purine derivative excretion in limit-fed dairy heifers[J]. Journal Dairy Science,2017,100(1):213-223
[37] KAMRA D N,SAHA S,BHATT N,et al. Effect of diet on enzyme profile,biochemical changes and in sacco degradability of feeds in the rumen of buffalo[J]. Asian-australasian Journal of Animal Science,2003,16(3):374-379
[38] ZHANG S,CHAUDHRY A S,RAMDANI D,et al.Chemical composition and in vitro fermentation characteristics of high sugar forage sorghum as an alternative to forage maize for silage making in Tarim Basin,China[J]. Journal of Integrative Agriculture,2016,15(1):175-182
[39] ZHAO S,YANG F,WANG Y,et al. Dynamics of fermentation parameters and bacterial community in high-moisture alfalfa silage with or without lactic acid bacteria[J]. Microorganisms,2021,9:1225

杏鲍菇菌糠和植物乳杆菌对全株玉米青贮有氧稳定性和体外发酵特性的影响

Effects of the Addition of Pleurotus eryngii Mushroom Bran and Lactobacillus Plantarum on the Aerobic Stability and In Vitro Fermentation Characteristics of Whole Corn Silage

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	金丽娜, 蒋苏苏, 敬淑燕, 何建文, 张慧君. 黄芪药渣与全株玉米混合青贮对饲料感官、发酵品质及营养成分的影响[J]. 草地学报, 2024, 32(2): 630-636.
[2]	杨丹, 吴硕, 陈丹丹, 张庆. 黄梁木精油活性成分测定及其对全株玉米青贮有氧稳定的影响[J]. 草地学报, 2023, 31(9): 2860-2867.
[3]	刘逸超, 孙鹏波, 张佳伟, 李冬梅, 马宏伟, 井志伟, 王瑞峰, 贾玉山. 发酵抑制剂对羊草青贮品质和有氧稳定性的影响[J]. 草地学报, 2023, 31(8): 2564-2570.
[4]	陈丹丹, 黄佩珊, 张超, 陈晓阳, 周玮, 张庆. 植物乳杆菌对荔枝叶青贮品质及体外降解特性的影响[J]. 草地学报, 2023, 31(7): 2232-2240.
[5]	冯启贤, 张磊, 李妍, 凌文卿, 李珏, 周燚, 阳伏林, 周晶. 甲酸处理对苜蓿青贮品质及瘤胃降解率的影响[J]. 草地学报, 2023, 31(4): 1264-1272.
[6]	陈三冬, 巩海强, 尹国丽, 师尚礼. 青贮玉米有氧暴露过程中营养品质及细菌群落多样性[J]. 草地学报, 2023, 31(2): 388-395.
[7]	巩海强, 柴继宽, 陈三冬, 尹国丽, 邓秀霞. 抑制黄曲霉乳酸菌的筛选与鉴定[J]. 草地学报, 2023, 31(2): 587-595.
[8]	高龙, 张娟, 戈辰妍, 刘宽, 唐嘉祺, 石神炳, 阳伏林, 周晶. 添加植物乳杆菌、布氏乳杆菌对杂交狼尾草青贮发酵品质及有氧稳定性的影响[J]. 草地学报, 2023, 31(12): 3867-3875.
[9]	郑杨晨, 赵峻祥, 刘宇洋, 卞彬彬, 孔令芝, 李井春, 李雁冰. 湿贮玉米中添加春蓼对饲料抑菌能力的影响[J]. 草地学报, 2023, 31(12): 3887-3896.
[10]	刘逸超, 司强, 刘明健, 卢轩, 闫蒙, 格根图, 王志军, 贾玉山. 装填密度对羊草和苜蓿青贮品质及有氧稳定性的影响[J]. 草地学报, 2023, 31(1): 263-271.
[11]	张佳伟, 贾玉山, 格根图, 王志军, 包健, 李宇宇, 彭冬, 王宇, 孙鹏波, 岳晓明. 不同丙酸浓度对天然牧草青贮品质及有氧稳定性的影响[J]. 草地学报, 2022, 30(9): 2522-2528.
[12]	付薇, 陈伟, 周丽, 蔡璐, 王小利, 韩永芬, 孟军江. 植物乳杆菌和短乳杆菌复合添加对高丹草青贮效果的影响[J]. 草地学报, 2022, 30(3): 758-763.
[13]	李彦飞, 杨双双, 初晓辉, 马向丽, 段新慧, 韩博, 段佳鑫, 单贵莲. 昆明优质本土乳酸菌的筛选及其对全株玉米青贮品质的影响[J]. 草地学报, 2022, 30(12): 3215-3224.
[14]	张嘉懿, 格根图, 贾玉山, 王志军, 张佳伟, 闫星全, 司强, 赵牧其尔, 孙林. 不同乳酸菌添加剂对苦荬菜青贮品质的影响[J]. 草地学报, 2022, 30(12): 3433-3438.
[15]	曹欣, 吴康乐, 文乐元, 黄丽娟, 肖润林, 张志飞. 水稻秸秆、麦麸、葡萄糖添加对绿狐尾藻青贮质量影响研究[J]. 草地学报, 2022, 30(12): 3447-3454.