结缕草属种质资源EST-SSR遗传多样性分析

doi:10.11733/j.issn.1007-0435.2024.08.006

草地学报 ›› 2024, Vol. 32 ›› Issue (8): 2386-2393.DOI: 10.11733/j.issn.1007-0435.2024.08.006

• 研究论文 • 上一篇

结缕草属种质资源EST-SSR遗传多样性分析

黄春琼¹, 何潇^1,2, 罗丽娟², 董荣书¹, 刘国道¹

1. 中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室, 海南海口 571101;
2. 海南大学热带作物学院, 海南海口 570228

收稿日期:2024-01-10 修回日期:2024-03-26 发布日期:2024-09-07
通讯作者: 罗丽娟,E-mail:luoljd@126.com
作者简介:黄春琼(1982-),女,汉族,云南宣威人,博士,研究员,主要从事热带草种质资源与育种研究,E-mail:huangchunqiong44@163.com
基金资助:
海南省自然科学基金(322RC770;323RC536);国家现代农业产业技术体系(CARS-34)资助

Genetic Diversity Analysis of Zoysia willd. Accessions by EST-SSR Markers

HUANG Chun-qiong¹, HE Xiao^1,2, LUO Li-juan², DONG Rong-shu¹, LIU Guo-dao¹

1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Haikouu, Hainan Province 571101, China;
2. College of Tropical Crops Hainan University, Haikou, Hainan Province 570228, China

Received:2024-01-10 Revised:2024-03-26 Published:2024-09-07

摘要/Abstract

摘要： 本研究以43份不同来源的结缕草属种质为材料，利用表达序列标签-简单重复序列(Expressed Sequence Tag-Simple sequence repeat，EST-SSR)引物对其进行遗传多样性分析。结果表明：从125对EST-SSR引物中筛选出30对能稳定扩增、条带清晰的引物；共检测到124个标记，平均每对引物检测到4个，多态性标记有108个，多态性位点百分率为88.60%；种质间遗传相似性系数为0.530～0.864，平均为0.711；共检测到104个观察等位基因，平均每对引物检测到3个；有效等位基因数范围为0～5，平均为3；Shannon信息指数变化范围为0～1.593，平均为0.940。Nei’s基因多样性指数范围为0～0.793，平均为0.541；在遗传相似性系数为0.800时，利用非加权组平均法(Unweighted pair-group method with arithmetic means,UPGMA)法将43份种质划分为4个类群，聚类结果与种质地理位置有一定的关联，但联系不紧密。本研究结果表明43份种质的遗传多样性处于上等水平，EST-SSR标记能有效地评价结缕草属种质的遗传差异，为结缕草属种质资源的鉴定评价及分子标记辅助育种提供参考依据。

关键词: 结缕草属, EST-SSR, 分子标记, 遗传多样性, 群体结构

Abstract: In the present study,43 Zoysia willd. accessions from different sources were used to detect the genetic diversity using Expressed sequence tag-simple sequence repeat(EST-SSR) primers. The results showed that 30 pairs of primer from the original 125 pairs of primer were selected based on the polymorphic,clear and reproducible bands amplified by them. A total of 124 bands were amplified by 30 primer pairs from 43 accessions with 4 bands per primer pairs,and 108 (88.60%) out of them were polymorphic. The genetic similarity coeffcients (GSC) among the 43 accessions ranged from 0.530 to 0.864 with an average of 0.711. A total of 104 observing alleles were amplified by 30 pairs of primer from 43 accessions with 3 observed alleles per pairs of primer. The range of effective number of alleles was 0 to 5 with an average of 3. The range of shannon information index was 0～1.593 with an average of 0.940. The Nei’s genetic diversity index was 0～0.793 with an average of 0.541. Cluster analysis conducted by the unweighted pair-group method with arithmetic averages (UPGMA) separated the 43 accessions into 4 groups when the genetics similarity coefficient was 0.800. The clustering results were somewhat correlated with the geographical location of the accessions,but not closely related. The results indicated that the genetic diversity of Zoysia willd. accessions was rich,which laid a foundation for the identification and evaluation of Zoysia willd. accessions and molecular marker-assisted breeding.

Key words: Zoysia willd, EST-SSR, Molecular makers, Geneic diversity, Population structure

中图分类号:

S543

黄春琼, 何潇, 罗丽娟, 董荣书, 刘国道. 结缕草属种质资源EST-SSR遗传多样性分析[J]. 草地学报, 2024, 32(8): 2386-2393.

HUANG Chun-qiong, HE Xiao, LUO Li-juan, DONG Rong-shu, LIU Guo-dao. Genetic Diversity Analysis of Zoysia willd. Accessions by EST-SSR Markers[J]. Acta Agrestia Sinica, 2024, 32(8): 2386-2393.

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参考文献

[1] 黄春琼,刘国道,白昌军,等. 结缕草属植物种质资源遗传变异研究[J]. 热带作物学报,2018,39(7):1259-1265
[2] 宣继萍. 结缕草属(Zoysia Willd.)植物种质资源多样性研究[D]. 南京:南京农业大学,2008:44
[3] 李亚,谢晓金,宣继萍.中国结缕草属(Zoysia spp.)植物抗寒性评价[J]. 草地学报,2003,11(3):240-245
[4] 李亚,耿蕾,刘建秀. 中国结缕草属植物抗盐性评价[J]. 草地学报,2004,12(1):8-11
[5] KIM J H. A taxonomic study of genus Zoysia Willd. in Korea[J]. Korean Journal of Plant Taxonom,1983,13(1):41-53
[6] ZOTOV V D. Zoysia Willd. (Gramineae) in New Zealand[J]. New Zealand Journal of Botany,1971,9(4):634-644
[7] 高雪. 三种草坪草EST-SSR分子标记开发及利用[D]. 济南:山东农业大学,2015:7
[8] 郑荣佳,孔维一,冯嘉欣,等. 结缕草属植物表型多样性分析及优异种质筛选[J]. 草地学报,2022,30(9):2325-2335
[9] GROVER A,SHARMA P C. Development and use of molecular markers:past and present[J]. Critical Reviews in Biotechnology,2016,36:290-302
[10] YANESHITA M,NAGASAWA R,ENGELKE M C,et al. Genetic variation and interspecific hybridization among natural populations of zoysia grasses detected by RFLP analyses of chloroplast and nuclear DNA[J]. Genes and Genetic Systems,1997,72(4):173-179
[11] CHOI J S,AHN B J,YANG G M. Classification of zoysia grasses (Zoysia spp.) native to the southwest coastal regions of Korea using RAPDs[J]. Journal Korean Society Horticultural Science,1997,38(6):789-795
[12] WENG J H,FAN M J,LIN C Y,et al. Genetic variation of Zoysia as revealed by random amplified polymorphic DNA(RAPD) and Isozyme pattern[J]. Plant Production Science,2007,10(1):80-85
[13] CAI H W,INOUE M,YUYAMA N,et al. An AFLP based linkage map of zoysiagrass (Zoysia japonica)[J]. Plant Breeding,2004,123(6):543-548
[14] CAI H W,INOUE M,YUYAMA N,et al. Isolation,characterization and mapping of simple sequence repeat markers in zoysia grass (Zoysia spp.)[J]. Theoretical and Applied Genetics,2005,112:158-166
[15] CHEN H M,LI L Z,WEI X Y,et al. Development,chromosome location genetic mapping of EST-SSR markers in wheat[J]. Chinese Science Bulletin,2005,50:2328-2336
[16] HOLTON T A,CHRISTOPHER J T,MCCLURE L,et al. Identification and mapping of polymorphic SSR markers expressed gene sequences of barley and wheat[J]. Molecular Breeding,2002,9:63-71
[17] SAHA M C,ROUF MIAN M A,EUJAYL I,et al. Tall fescue EST-SSR markers with transferability across several grass species[J]. Theoretical and Applied Genetics,2004,109:783-791
[18] ZHANG L Y,BERNARD M,LEROY P,et al. High transferability of bread wheat EST-derived SSRs to other cereals[J]. Theoretical and Applied Genetics,2005,111:677-687
[19] ZHANG L Y,RAVEL C,BERNARD M,et al. Transferable bread wheat EST-SSRs can be useful for phylogenetic,studies among the triticeae species[J]. Theoretical and Applied Genetics,2006,113:407-418
[20] YAN H,ZHANG Y,ZENG B,et al. Genetic diversity and association of EST-SSR and SCoT markers with rust traits in Orchardgrass (Dactylis glomerata L.)[J]. Molecules,2016,21(1),66
[21] 赵岩,丁承强,孔凡美,等. EST-SSR标记在结缕草中的通用性[J]. 中国草地学报,2008,30(3):69-73
[22] HUANG C Q,HUANG D Y,ZHANG Y F,et al. Genetic analysis for 57 accessions of Cynodon dactylon from 17 countries in 6 continents by SRAP markers[J]. Tropical grasslands,2010,44:274-281
[23] ZHANG J X,WANG M L,GUO Z P,et al. Genetic diversity and population structure of bermudagrass [Cynodon dactylon (L.) Pers.] along latitudinal gradients and the relationship with polyploidy level[J]. Diversity,2019,11(8):135
[24] BRIAN M S,JEFF A B,KAREN R,et al. Development,linkage mapping,and use of microsatellites in bermudagrass[J]. Journal of the American Society for Horticultural Science,2010,135(6):511-520
[25] TERRY L K,NEIL R W,VICTOR M O,et al. DNA polymorphisms at bermudagrass microsatellite loci and their use in genotype fingerprinting[J]. Crop Science,2011,51(3):1122-1131
[26] 崔蓉菁. 狗牙根耐铝种质的筛选及耐铝性状与标记的关联分析[D]. 南京:南京农业大学,2020:46
[27] WENG J H. Genetic variation of Zoysia in Taiwan as analyzed by isozyme patterns and salinity tolerance[J]. Plant Production Science,2002,5(3):236-241
[28] WENG J H,FAN M J,LIN C Y,et al. Genetic variation of Zoysia as revealed by random amplified polymorphic DNA(RAPD)and isozyme pattern[J]. Plant Production Science,2007,10(1):80-85
[29] 郭海林. 结缕草属植物种质资源多样性及重要性状的遗传分析与分子标记[D]. 南京:南京农业大学,2008:42-43
[30] ODENY D A,JAYASHREE B,GEBHARDT C,et al. New microsatellite markers for pigeonpea (Cajanus cajan (L.) millsp.)[J]. BMC Research Notes,2009,2(1):1-5
[31] DUTTA S,KUMAWAT,G,SINGH,B P,et al. Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh][J]. BMC Plant Biology,2011,11(1):17
[32] 郭海林,刘建秀,周志芳,等. 结缕草属植物种间关系和遗传多样性的SSR标记分析[J]. 草地学报,2008,16(6):552-558
[33] 郭海林,郑轶琦,陈宣,等. 结缕草属植物种间关系和遗传多样性的SRAP标记分析[J]. 草业学报,2009,18(5):201-210
[34] 陈志祥,罗小燕,李拴林,等. 基于SSR标记的木豆种质资源遗传多样性与群体结构分析[J].草地学报,2021,29(5):904-91
[35] 伊然,王子玥,刘凌云,等. 基于EST-SSR标记的60份苇状羊茅遗传多样性分析[J]. 草地学报,2023,31(7):2041-2048
[36] 陈斐,魏臻武,李伟民,等. 基于SSR标记的苜蓿种质资源遗传多样性与群体结构分析[J]. 草地学报,2013,21(4):759-768
[37] 曾美娟,刘建汀,卓玲玲,等. 茄子EST-SSR分子标记的鉴定及多态性分析[J]. 中国细胞生物学学报,2020,42(8):1366-1373
[38] 董乐,田仟仟,黄彬,等. 浙江红花油茶经济性状与SSR分子标记的关联分析[J]. 分子植物育种,2022,20(14):4710-4722(责任编辑闵芝智)第32卷第8期 Vol.32 No. 8草地学报 ACTAAGRESTIASINICA 2024年 8月

结缕草属种质资源EST-SSR遗传多样性分析

Genetic Diversity Analysis of Zoysia willd. Accessions by EST-SSR Markers

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